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Protein

UDP-N-acetylmuramoylalanine--D-glutamate ligase

Gene

murD

Organism
Staphylococcus aureus (strain MSSA476)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA).UniRule annotation

Catalytic activityi

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanine + D-glutamate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate.UniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi118 – 124ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligaseUniRule annotation (EC:6.3.2.9UniRule annotation)
Alternative name(s):
D-glutamic acid-adding enzymeUniRule annotation
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetaseUniRule annotation
Gene namesi
Name:murDUniRule annotation
Ordered Locus Names:SAS1117
OrganismiStaphylococcus aureus (strain MSSA476)
Taxonomic identifieri282459 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001090861 – 449UDP-N-acetylmuramoylalanine--D-glutamate ligaseAdd BLAST449

Structurei

3D structure databases

ProteinModelPortaliQ6GA29.
SMRiQ6GA29.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MurCDEF family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000049428.
KOiK01925.
OMAiPGIPYTN.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.50.720. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_00639. MurD. 1 hit.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR016040. NAD(P)-bd_dom.
IPR005762. UDP-N-AcMur-Glu_ligase.
[Graphical view]
PfamiPF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01087. murD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6GA29-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNYTGLENK NVLVVGLAKS GYEAAKLLSK LGANVTVNDG KDLSQDAHAK
60 70 80 90 100
DLESMGISVV SGSHPLTLLD NNPIIVKNPG IPYTVSIIDE AVKRGLKILT
110 120 130 140 150
EVELSYLISE APIIAVTGTN GKTTVTSLIG DMFKKSRLTG RLSGNIGYVA
160 170 180 190 200
SKVAQEVKPT DYLVTELSSF QLLGIEKYKP HIAIITNIYS AHLDYHENLE
210 220 230 240 250
NYQNAKKQIY KNQTEEDYLI CNYHQRQVIE SEELKAKTLY FSTQQEVDGI
260 270 280 290 300
YIKDGFIIYK GVRIINTEDL VLPGEHNLEN ILAAVLACIL AGVPIKAIID
310 320 330 340 350
SLTTFSGIEH RLQYVGTNRT NKYYNDSKAT NTLATQFALN SFNQPIIWLC
360 370 380 390 400
GGLDRGNEFD ELIPYMENVR AMVVFGQTKA KFAKLGNSQG KSVIEANNVE
410 420 430 440
DAVDKVQDII EPNDVVLLSP ACASWDQYST FEERGEKFIE RFRAHLPSY
Length:449
Mass (Da):49,858
Last modified:July 19, 2004 - v1
Checksum:iD681C540EEE4EB18
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571857 Genomic DNA. Translation: CAG42894.1.
RefSeqiWP_000935989.1. NC_002953.3.

Genome annotation databases

KEGGisas:SAS1117.
PATRICi19551689. VBIStaAur96780_1162.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571857 Genomic DNA. Translation: CAG42894.1.
RefSeqiWP_000935989.1. NC_002953.3.

3D structure databases

ProteinModelPortaliQ6GA29.
SMRiQ6GA29.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGisas:SAS1117.
PATRICi19551689. VBIStaAur96780_1162.

Phylogenomic databases

HOGENOMiHOG000049428.
KOiK01925.
OMAiPGIPYTN.

Enzyme and pathway databases

UniPathwayiUPA00219.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.50.720. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_00639. MurD. 1 hit.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR016040. NAD(P)-bd_dom.
IPR005762. UDP-N-AcMur-Glu_ligase.
[Graphical view]
PfamiPF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01087. murD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMURD_STAAS
AccessioniPrimary (citable) accession number: Q6GA29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: July 19, 2004
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.