Reviewed, UniProtKB/Swiss-Prot Q6G9C3 (DHA1_STAAS)

Names and origin

Protein names

Recommended name:
    Alanine dehydrogenase 1
    EC=1.4.1.1

Gene names

Name:	ald1
Ordered Locus Names:	SAS1382

Organism

Staphylococcus aureus (strain MSSA476) [Complete proteome] [HAMAP]

Taxonomic identifier

282459 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer By similarity.
Catalytic activity	L-alanine + H(2)O + NAD(+) = pyruvate + NH(3) + NADH.
Pathway	Amino-acid degradation; L-alanine degradation via dehydrogenase pathway; NH(3) and pyruvate from L-alanine: step 1/1.
Sequence similarities	Belongs to the AlaDH/PNT family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine dehydrogenase activity Inferred from electronic annotation. Source: InterPro binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Alanine dehydrogenase 1		PRO_0000198999
Regions
	Nucleotide binding	170 – 200	31	NAD By similarity
Sites
	Active site	94	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6G9C3-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 7FF131E5E18D4035	FASTA	372	40,234
	10 20 30 40 50 60 MLVAVVKELK QGEGRVACTP ENVRKLTDAG HKVIVEKNAG IGSGFSNDMY EKEGAKIVTH 70 80 90 100 110 120 EQAWEADLVI KVKEPHESEY QYFKKNQIIW GFLHLASSKE IVEKMQEVGV TAISGETIIK 130 140 150 160 170 180 NGKAELLAPM SAIAGQRSAI MGAYYSEAQH GGQGTLVTGV HENVDIPGST YVIFGGGVAA 190 200 210 220 230 240 TNAANVALGL NAKVIIIELN DDRIKYLQDM YAEKDVTVVK STPENLAEQI KKADVFISTI 250 260 270 280 290 300 LIPGAKPPKL VTREMVKSMK KGSVLIDIAI DQGGTIETIR PTTISDPVYE EEGVIHYGVP 310 320 330 340 350 360 NQPGAVPRTS TMALAQGNID YILEICDKGL EQAIKDNEAL STGVNIYQGQ VTNQGLASSH 370 DLDYKEILNV IE

References

[1]

"Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance." Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J., Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A., Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C., Clark L., Corton C. , Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z., Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N., Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M., Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004) [PubMed: 15213324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX571857 Genomic DNA. Translation: CAG43158.1.
RefSeq	YP_043500.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2863854.
GenomeReviews	Gene locus SAS1382 in contig BX571857_GR.
KEGG	sas:SAS1382.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6G9C3.
Enzyme and pathway databases
BioCyc	SAUR282459:SAS1382-MON.
Family and domain databases
InterPro	IPR007698. Ala_DHase/PNT_C. IPR008142. Ala_DHase/PNT_CS1. IPR008143. Ala_DHase/PNT_CS2. IPR007886. Ala_DHase/PNT_N. IPR008141. Ala_DHase_PNT. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01262. AlaDh_PNT_C. 1 hit. PF05222. AlaDh_PNT_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00518. alaDH. 1 hit.
PROSITE	PS00836. ALADH_PNT_1. 1 hit. PS00837. ALADH_PNT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHA1_STAAS

Accession

Primary (citable) accession number: Q6G9C3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	July 19, 2004
Last modified:	November 25, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)