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Q6G8J7

- ISDH_STAAS

UniProt

Q6G8J7 - ISDH_STAAS

Protein

Iron-regulated surface determinant protein H

Gene

isdH

Organism
Staphylococcus aureus (strain MSSA476)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (19 Jul 2004)
      Previous versions | rss
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    Functioni

    Binds human plasma haptoglobin-hemoglobin complexes, haptoglobin and hemoglobin. Binds haptoglobin-hemoglobin complexes with significantly higher affinity than haptoglobin alone Probable.Curated

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Iron-regulated surface determinant protein H
    Alternative name(s):
    Haptoglobin receptor A
    Staphylococcus aureus surface protein I
    Gene namesi
    Name:isdH
    Synonyms:harA, sasI
    Ordered Locus Names:SAS1657
    OrganismiStaphylococcus aureus (strain MSSA476)
    Taxonomic identifieri282459 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000002201: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cell wall Source: UniProtKB-SubCell
    2. extracellular region Source: UniProtKB-KW
    3. membrane Source: InterPro

    Keywords - Cellular componenti

    Cell wall, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 4040Sequence AnalysisAdd
    BLAST
    Chaini41 – 864824Iron-regulated surface determinant protein HPRO_0000285185Add
    BLAST
    Propeptidei865 – 89531Removed by sortaseSequence AnalysisPRO_0000285186Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei864 – 8641Pentaglycyl murein peptidoglycan amidated threonineSequence Analysis

    Keywords - PTMi

    Peptidoglycan-anchor

    Interactioni

    Protein-protein interaction databases

    STRINGi282459.SAS1657.

    Structurei

    Secondary structure

    1
    895
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni86 – 883
    Helixi90 – 934
    Helixi97 – 993
    Beta strandi109 – 1124
    Beta strandi114 – 1174
    Beta strandi121 – 1233
    Helixi125 – 1306
    Beta strandi133 – 1386
    Beta strandi141 – 15111
    Turni153 – 1553
    Beta strandi156 – 16510
    Beta strandi166 – 1683
    Beta strandi171 – 1766
    Turni178 – 1803
    Beta strandi182 – 1898
    Beta strandi193 – 20311
    Beta strandi205 – 2073
    Beta strandi209 – 2124
    Beta strandi216 – 2238

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2H3KNMR-A86-229[»]
    3OVUX-ray2.83B86-229[»]
    3S48X-ray3.05A/B86-229[»]
    ProteinModelPortaliQ6G8J7.
    SMRiQ6G8J7. Positions 86-229, 326-467, 542-657.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ6G8J7.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini105 – 232128NEAT 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini345 – 471127NEAT 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini543 – 660118NEAT 3PROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi861 – 8655LPXTG sorting signalSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi698 – 77982Asp-richAdd
    BLAST

    Domaini

    The NEAT 1 domain binds with higher affinity than the NEAT 2 domain haptoglobin-hemoglobin complexes, haptoglobin and hemoglobin By similarity. More than one IsdH NEAT 1 domain interacts with a single hemoglobin.By similarity

    Sequence similaritiesi

    Belongs to the IsdH family.Curated
    Contains 3 NEAT domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG12793.
    HOGENOMiHOG000280077.
    OMAiVQTGQED.
    OrthoDBiEOG6RNQ8T.

    Family and domain databases

    InterProiIPR019930. Haptoglobin-bd_HarA.
    IPR019931. LPXTG_anchor.
    IPR006635. NEAT_dom.
    IPR005877. YSIRK_signal_dom.
    [Graphical view]
    PfamiPF05031. NEAT. 3 hits.
    PF04650. YSIRK_signal. 1 hit.
    [Graphical view]
    SMARTiSM00725. NEAT. 3 hits.
    [Graphical view]
    TIGRFAMsiTIGR03658. IsdH_HarA. 1 hit.
    TIGR01167. LPXTG_anchor. 1 hit.
    TIGR01168. YSIRK_signal. 1 hit.
    PROSITEiPS50978. NEAT. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q6G8J7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNKHHPKLRS FYSIRKSILG VASVIVSTLF LITSQHQAQA AENTNTSDKI    50
    SENQNNNATT TQPPKDTNQT QPATQPANTA KTYPAADESL KDAIKDPALE 100
    NKEHDIGPRE QVNFQLLDKN NETQYYHFFS IKDPADVYYT KKKAEVELDI 150
    NTASTWKKFE VYENNQKLPV RLVSYSPVPE DHAYIRFPVS DGTQELKIVS 200
    STQIDDGEET NYDYTKLVFA KPIYNDPSLV KSDTNDAVVT NDQSSSDASN 250
    QTNTNTSNQN TSTINNANNQ PQATTNMSQP AQPKSSANAD QASSQPAHET 300
    NSNGNTNDKT NESSNQSDVN QQYPPADESL QDAIKNPAII DKEHTADNWR 350
    PIDFQMKNDK GERQFYHYAS TVEPATVIFT KTGPIIELGL KTASTWKKFE 400
    VYEGDKKLPV ELVSYDSDKD YAYIRFPVSN GTREVKIVSS IEYGENIHED 450
    YDYTLMVFAQ PITNNPDDYV DEETYNLQKL LAPYHKAKTL ERQVYELEKL 500
    QEKLPEKYKA EYKKKLDQTR VELADQVKSA VTEFENVTPT NDQLTDVQEA 550
    HFVVFESEEN SESVMDGFVE HPFYTATLNG QKYVVMKTKD DSYWKDLIVE 600
    GKRVTTVSKD PKNNSRTLIF PYIPDKAVYN AIVKVVVANI GYEGQYHVRI 650
    INQDINTKDD DTSQNNTSEP LNVQTGQEGK VADTDVAENS STATNPKDAS 700
    DKADVIEPDS DVVKDADNNI DKDVQHDVDH LSDMSDNNHF DKYDLKEMDT 750
    QIAKDTDRNV DKGADNSVGM SSNVDTDKDS NKNKDKVIQL NHIADKNNHN 800
    GKAAKLDVVK QNYNNTDKVT DKKTTEHLPS DIHKTVDKTV KTKEKAGTPS 850
    KENKLSQSKM LPKTGETTSS QSWWGLYALL GMLALFIPKF RKESK 895
    Length:895
    Mass (Da):100,847
    Last modified:July 19, 2004 - v1
    Checksum:i797BB0D67F9A0559
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX571857 Genomic DNA. Translation: CAG43459.1.
    RefSeqiYP_043776.1. NC_002953.3.

    Genome annotation databases

    GeneIDi2863914.
    KEGGisas:SAS1657.
    PATRICi19552805. VBIStaAur96780_1721.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX571857 Genomic DNA. Translation: CAG43459.1 .
    RefSeqi YP_043776.1. NC_002953.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2H3K NMR - A 86-229 [» ]
    3OVU X-ray 2.83 B 86-229 [» ]
    3S48 X-ray 3.05 A/B 86-229 [» ]
    ProteinModelPortali Q6G8J7.
    SMRi Q6G8J7. Positions 86-229, 326-467, 542-657.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 282459.SAS1657.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2863914.
    KEGGi sas:SAS1657.
    PATRICi 19552805. VBIStaAur96780_1721.

    Phylogenomic databases

    eggNOGi NOG12793.
    HOGENOMi HOG000280077.
    OMAi VQTGQED.
    OrthoDBi EOG6RNQ8T.

    Miscellaneous databases

    EvolutionaryTracei Q6G8J7.

    Family and domain databases

    InterProi IPR019930. Haptoglobin-bd_HarA.
    IPR019931. LPXTG_anchor.
    IPR006635. NEAT_dom.
    IPR005877. YSIRK_signal_dom.
    [Graphical view ]
    Pfami PF05031. NEAT. 3 hits.
    PF04650. YSIRK_signal. 1 hit.
    [Graphical view ]
    SMARTi SM00725. NEAT. 3 hits.
    [Graphical view ]
    TIGRFAMsi TIGR03658. IsdH_HarA. 1 hit.
    TIGR01167. LPXTG_anchor. 1 hit.
    TIGR01168. YSIRK_signal. 1 hit.
    PROSITEi PS50978. NEAT. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MSSA476.
    2. "Solution structure of the NEAT (NEAr Transporter) domain from IsdH/HarA: the human hemoglobin receptor in Staphylococcus aureus."
      Pilpa R.M., Fadeev E.A., Villareal V.A., Wong M.L., Phillips M., Clubb R.T.
      J. Mol. Biol. 360:435-447(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 86-229, BINDING TO HEMOGLOBIN.

    Entry informationi

    Entry nameiISDH_STAAS
    AccessioniPrimary (citable) accession number: Q6G8J7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 2007
    Last sequence update: July 19, 2004
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3