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Protein

Iron-regulated surface determinant protein H

Gene

isdH

Organism
Staphylococcus aureus (strain MSSA476)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Binds human plasma haptoglobin-hemoglobin complexes, haptoglobin and hemoglobin. Binds haptoglobin-hemoglobin complexes with significantly higher affinity than haptoglobin alone (Probable).Curated

Names & Taxonomyi

Protein namesi
Recommended name:
Iron-regulated surface determinant protein H
Alternative name(s):
Haptoglobin receptor A
Staphylococcus aureus surface protein I
Gene namesi
Name:isdH
Synonyms:harA, sasI
Ordered Locus Names:SAS1657
OrganismiStaphylococcus aureus (strain MSSA476)
Taxonomic identifieri282459 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000002201: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cell wall Source: UniProtKB-SubCell
  2. extracellular region Source: UniProtKB-KW
  3. membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4040Sequence AnalysisAdd
BLAST
Chaini41 – 864824Iron-regulated surface determinant protein HPRO_0000285185Add
BLAST
Propeptidei865 – 89531Removed by sortaseSequence AnalysisPRO_0000285186Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei864 – 8641Pentaglycyl murein peptidoglycan amidated threonineSequence Analysis

Keywords - PTMi

Peptidoglycan-anchor

Interactioni

Protein-protein interaction databases

STRINGi282459.SAS1657.

Structurei

Secondary structure

1
895
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni86 – 883Combined sources
Helixi90 – 934Combined sources
Helixi97 – 993Combined sources
Beta strandi109 – 1124Combined sources
Beta strandi114 – 1174Combined sources
Beta strandi121 – 1233Combined sources
Helixi125 – 1306Combined sources
Beta strandi133 – 1386Combined sources
Beta strandi141 – 15111Combined sources
Turni153 – 1553Combined sources
Beta strandi156 – 16510Combined sources
Beta strandi166 – 1683Combined sources
Beta strandi171 – 1766Combined sources
Turni178 – 1803Combined sources
Beta strandi182 – 1898Combined sources
Beta strandi193 – 20311Combined sources
Beta strandi205 – 2073Combined sources
Beta strandi209 – 2124Combined sources
Beta strandi216 – 2238Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2H3KNMR-A86-229[»]
3OVUX-ray2.83B86-229[»]
3S48X-ray3.05A/B86-229[»]
ProteinModelPortaliQ6G8J7.
SMRiQ6G8J7. Positions 86-229, 326-467, 542-657.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6G8J7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini105 – 232128NEAT 1PROSITE-ProRule annotationAdd
BLAST
Domaini345 – 471127NEAT 2PROSITE-ProRule annotationAdd
BLAST
Domaini543 – 660118NEAT 3PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi861 – 8655LPXTG sorting signalSequence Analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi698 – 77982Asp-richAdd
BLAST

Domaini

The NEAT 1 domain binds with higher affinity than the NEAT 2 domain haptoglobin-hemoglobin complexes, haptoglobin and hemoglobin (By similarity). More than one IsdH NEAT 1 domain interacts with a single hemoglobin.By similarity

Sequence similaritiesi

Belongs to the IsdH family.Curated
Contains 3 NEAT domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG12793.
HOGENOMiHOG000280077.
OMAiVQTGQED.
OrthoDBiEOG6RNQ8T.

Family and domain databases

InterProiIPR019930. Haptoglobin-bd_HarA.
IPR019931. LPXTG_anchor.
IPR006635. NEAT_dom.
IPR005877. YSIRK_signal_dom.
[Graphical view]
PfamiPF05031. NEAT. 3 hits.
PF04650. YSIRK_signal. 1 hit.
[Graphical view]
SMARTiSM00725. NEAT. 3 hits.
[Graphical view]
TIGRFAMsiTIGR03658. IsdH_HarA. 1 hit.
TIGR01167. LPXTG_anchor. 1 hit.
TIGR01168. YSIRK_signal. 1 hit.
PROSITEiPS50978. NEAT. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6G8J7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKHHPKLRS FYSIRKSILG VASVIVSTLF LITSQHQAQA AENTNTSDKI
60 70 80 90 100
SENQNNNATT TQPPKDTNQT QPATQPANTA KTYPAADESL KDAIKDPALE
110 120 130 140 150
NKEHDIGPRE QVNFQLLDKN NETQYYHFFS IKDPADVYYT KKKAEVELDI
160 170 180 190 200
NTASTWKKFE VYENNQKLPV RLVSYSPVPE DHAYIRFPVS DGTQELKIVS
210 220 230 240 250
STQIDDGEET NYDYTKLVFA KPIYNDPSLV KSDTNDAVVT NDQSSSDASN
260 270 280 290 300
QTNTNTSNQN TSTINNANNQ PQATTNMSQP AQPKSSANAD QASSQPAHET
310 320 330 340 350
NSNGNTNDKT NESSNQSDVN QQYPPADESL QDAIKNPAII DKEHTADNWR
360 370 380 390 400
PIDFQMKNDK GERQFYHYAS TVEPATVIFT KTGPIIELGL KTASTWKKFE
410 420 430 440 450
VYEGDKKLPV ELVSYDSDKD YAYIRFPVSN GTREVKIVSS IEYGENIHED
460 470 480 490 500
YDYTLMVFAQ PITNNPDDYV DEETYNLQKL LAPYHKAKTL ERQVYELEKL
510 520 530 540 550
QEKLPEKYKA EYKKKLDQTR VELADQVKSA VTEFENVTPT NDQLTDVQEA
560 570 580 590 600
HFVVFESEEN SESVMDGFVE HPFYTATLNG QKYVVMKTKD DSYWKDLIVE
610 620 630 640 650
GKRVTTVSKD PKNNSRTLIF PYIPDKAVYN AIVKVVVANI GYEGQYHVRI
660 670 680 690 700
INQDINTKDD DTSQNNTSEP LNVQTGQEGK VADTDVAENS STATNPKDAS
710 720 730 740 750
DKADVIEPDS DVVKDADNNI DKDVQHDVDH LSDMSDNNHF DKYDLKEMDT
760 770 780 790 800
QIAKDTDRNV DKGADNSVGM SSNVDTDKDS NKNKDKVIQL NHIADKNNHN
810 820 830 840 850
GKAAKLDVVK QNYNNTDKVT DKKTTEHLPS DIHKTVDKTV KTKEKAGTPS
860 870 880 890
KENKLSQSKM LPKTGETTSS QSWWGLYALL GMLALFIPKF RKESK
Length:895
Mass (Da):100,847
Last modified:July 19, 2004 - v1
Checksum:i797BB0D67F9A0559
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571857 Genomic DNA. Translation: CAG43459.1.
RefSeqiYP_043776.1. NC_002953.3.

Genome annotation databases

GeneIDi2863914.
KEGGisas:SAS1657.
PATRICi19552805. VBIStaAur96780_1721.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX571857 Genomic DNA. Translation: CAG43459.1.
RefSeqiYP_043776.1. NC_002953.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2H3KNMR-A86-229[»]
3OVUX-ray2.83B86-229[»]
3S48X-ray3.05A/B86-229[»]
ProteinModelPortaliQ6G8J7.
SMRiQ6G8J7. Positions 86-229, 326-467, 542-657.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi282459.SAS1657.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2863914.
KEGGisas:SAS1657.
PATRICi19552805. VBIStaAur96780_1721.

Phylogenomic databases

eggNOGiNOG12793.
HOGENOMiHOG000280077.
OMAiVQTGQED.
OrthoDBiEOG6RNQ8T.

Miscellaneous databases

EvolutionaryTraceiQ6G8J7.

Family and domain databases

InterProiIPR019930. Haptoglobin-bd_HarA.
IPR019931. LPXTG_anchor.
IPR006635. NEAT_dom.
IPR005877. YSIRK_signal_dom.
[Graphical view]
PfamiPF05031. NEAT. 3 hits.
PF04650. YSIRK_signal. 1 hit.
[Graphical view]
SMARTiSM00725. NEAT. 3 hits.
[Graphical view]
TIGRFAMsiTIGR03658. IsdH_HarA. 1 hit.
TIGR01167. LPXTG_anchor. 1 hit.
TIGR01168. YSIRK_signal. 1 hit.
PROSITEiPS50978. NEAT. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MSSA476.
  2. "Solution structure of the NEAT (NEAr Transporter) domain from IsdH/HarA: the human hemoglobin receptor in Staphylococcus aureus."
    Pilpa R.M., Fadeev E.A., Villareal V.A., Wong M.L., Phillips M., Clubb R.T.
    J. Mol. Biol. 360:435-447(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 86-229, BINDING TO HEMOGLOBIN.

Entry informationi

Entry nameiISDH_STAAS
AccessioniPrimary (citable) accession number: Q6G8J7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: July 19, 2004
Last modified: January 7, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.