Q6G820 (NADE_STAAS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	SAS1836

Organism

Staphylococcus aureus (strain MSSA476) [Complete proteome] [HAMAP]

Taxonomic identifier

282459 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152198
Regions
	Nucleotide binding	47 – 54	8	ATP By similarity
Sites
	Active site	49	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6G820 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: EB4A022F38247F08	FASTA	273	30,683
	10 20 30 40 50 60 MSKLQDVIVQ EMKVKKRIDS AEEIMELKQF IKNYVQSHSF IKSLVLGISG GQDSTLVGKL 70 80 90 100 110 120 VQMSVNELRE EGIDCTFIAV KLPYGVQKDA DEVDQALRFI EPDEIVTVNI KPAVDQSVQS 130 140 150 160 170 180 LKEAGIVLTD FQKGNEKARE RMKVQFSIAS NRQGIVVGTD HSAENITGFY TKYGDGAADI 190 200 210 220 230 240 APIFGLNKRQ GRQLLAYLGA PKELYEKTPT ADLEDDKPQL PDEDALGVTY EAIDNYLEGK 250 260 270 PVTPEEQKVI ENHYIRNAHK RELAYTRYTW PKS

References

[1]

"Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance." Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J., Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A., Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C., Clark L., Corton C. , Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z., Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N., Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M., Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004) [PubMed: 15213324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MSSA476.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571857 Genomic DNA. Translation: CAG43641.1.
RefSeq	YP_043953.1. NC_002953.3.
3D structure databases
ProteinModelPortal	Q6G820.
SMR	Q6G820. Positions 4-268.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6G820.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000024361; EBSTAP00000023528; EBSTAG00000024360.
GeneID	2862133.
GenomeReviews	Gene locus SAS1836 in contig BX571857_GR.
KEGG	sas:SAS1836.
PATRIC	19553263. VBIStaAur96780_1913.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0171.
GeneTree	EBGT00050000025036.
HOGENOM	HBG351567.
OMA	IAQYEIA.
ProtClustDB	PRK00768.
Enzyme and pathway databases
BioCyc	SAUR282459:SAS1836-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_STAAS

Accession

Primary (citable) accession number: Q6G820

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program