Q6G7Q2 (ILVC_STAAS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacyl reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	SAS1961

Organism

Staphylococcus aureus (strain MSSA476) [Complete proteome] [HAMAP]

Taxonomic identifier

282459 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP-Rule MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP-Rule MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP-Rule MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP-Rule MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Ketol-acid reductoisomerase HAMAP-Rule MF_00435		PRO_0000151359
Sites
	Active site	107	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6G7Q2 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: D6B48BE5B5598859	FASTA	334	36,984
	10 20 30 40 50 60 MTTVYYDQDV KTDALQGKKI AVVGYGSQGH AHAQNLKDNG YDVVIGIRPG RSFDKAKEDG 70 80 90 100 110 120 FDVFPVAEAV KQADVIMVLL PDEIQGDVYK NEIEPNLEKH NALAFAHGFN IHFGVIQPPA 130 140 150 160 170 180 DVDVFLVAPK GPGHLVRRTF VEGSAVPSLF GIQQDASGQA RNIALSYAKG IGATRAGVIE 190 200 210 220 230 240 TTFKEETETD LFGEQAVLCG GVSKLIQSGF ETLVEAGYQP ELAYFEVLHE MKLIVDLMYE 250 260 270 280 290 300 GGTENVRYSI SNTAEFGDYV SGPRVITPDV KENMKAVLTD IQNGNFSNRF IEDNKNGFKE 310 320 330 FYKLREEQHG HQIEKVGREL REMMPFIKSK SIEK

References

[1]

"Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance." Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J., Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A., Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C., Clark L., Corton C. , Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z., Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N., Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M., Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MSSA476.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571857 Genomic DNA. Translation: CAG43768.1.
RefSeq	YP_044071.1. NC_002953.3.
3D structure databases
ProteinModelPortal	Q6G7Q2.
SMR	Q6G7Q2. Positions 3-327.
ModBase	Search...
Protein-protein interaction databases
STRING	282459.SAS1961.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2861757.
KEGG	sas:SAS1961.
PATRIC	19553521. VBIStaAur96780_2042.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0059.
HOGENOM	HOG000016230.
KO	K00053.
OMA	MAYAKGI.
ProtClustDB	PRK05479.
Enzyme and pathway databases
UniPathway	UPA00047; UER00056. UPA00049; UER00060.
Family and domain databases
Gene3D	1.10.1040.10. 1 hit.
HAMAP	MF_00435. IlvC.
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
PANTHER	PTHR21371. PTHR21371. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_STAAS

Accession

Primary (citable) accession number: Q6G7Q2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	July 19, 2004
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program