ID   PTLCB_STAAS             Reviewed;         570 AA.
AC   Q6G7C4;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   16-JUN-2009, entry version 37.
DE   RecName: Full=PTS system lactose-specific EIICB component;
DE   AltName: Full=EIICB-Lac;
DE            Short=EII-Lac;
DE   Includes:
DE     RecName: Full=Lactose permease IIC component;
DE     AltName: Full=PTS system lactose-specific EIIC component;
DE   Includes:
DE     RecName: Full=Lactose-specific phosphotransferase enzyme IIB component;
DE              EC=2.7.1.69;
DE     AltName: Full=PTS system lactose-specific EIIB component;
GN   Name=lacE; OrderedLocusNames=SAS2091;
OS   Staphylococcus aureus (strain MSSA476).
OC   Bacteria; Firmicutes; Bacillales; Staphylococcus.
OX   NCBI_TaxID=282459;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15213324; DOI=10.1073/pnas.0402521101;
RA   Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J.,
RA   Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A.,
RA   Bason N., Bentley S.D., Chillingworth C., Chillingworth T.,
RA   Churcher C., Clark L., Corton C., Cronin A., Doggett J., Dowd L.,
RA   Feltwell T., Hance Z., Harris B., Hauser H., Holroyd S., Jagels K.,
RA   James K.D., Lennard N., Line A., Mayes R., Moule S., Mungall K.,
RA   Ormond D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Sanders M.,
RA   Sharp S., Simmonds M., Stevens K., Whitehead S., Barrell B.G.,
RA   Spratt B.G., Parkhill J.;
RT   "Complete genomes of two clinical Staphylococcus aureus strains:
RT   evidence for the rapid evolution of virulence and drug resistance.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004).
CC   -!- FUNCTION: The phosphoenolpyruvate-dependent sugar
CC       phosphotransferase system (sugar PTS), a major carbohydrate active
CC       -transport system, catalyzes the phosphorylation of incoming sugar
CC       substrates concomitantly with their translocation across the cell
CC       membrane. This system is involved in lactose transport.
CC   -!- CATALYTIC ACTIVITY: Protein EIIB N(pi)-phospho-L-
CC       histidine/cysteine + sugar = protein EIIB + sugar phosphate.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein
CC       (Probable).
CC   -!- DOMAIN: The EIIC domain forms the PTS system translocation channel
CC       and contains the specific substrate-binding site.
CC   -!- DOMAIN: The EIIB domain is phosphorylated by phospho-EIIA on a
CC       cysteinyl or histidyl residue, depending on the transported sugar.
CC       Then, it transfers the phosphoryl group to the sugar substrate
CC       concomitantly with the sugar uptake processed by the EIIC domain.
CC   -!- SIMILARITY: Contains 1 PTS EIIB type-3 domain.
CC   -!- SIMILARITY: Contains 1 PTS EIIC type-3 domain.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; BX571857; CAG43899.1; -; Genomic_DNA.
DR   RefSeq; YP_044200.1; -.
DR   GeneID; 2863141; -.
DR   GenomeReviews; BX571857_GR; SAS2091.
DR   KEGG; sas:SAS2091; -.
DR   HOGENOM; Q6G7C4; -.
DR   OMA; Q6G7C4; WSKDIEN.
DR   BioCyc; SAUR282459:SAS2091-MON; -.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosph...; IEA:EC.
DR   GO; GO:0005529; F:sugar binding; IEA:InterPro.
DR   GO; GO:0005351; F:sugar:hydrogen symporter activity; IEA:UniProtKB-KW.
DR   GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phospho...; IEA:UniProtKB-KW.
DR   InterPro; IPR014350; PTrfase_system_EIIB_3_subgr.
DR   InterPro; IPR013012; PTS_EIIB_3.
DR   InterPro; IPR003352; PTS_EIIC.
DR   InterPro; IPR003501; PTS_IIB_lac.
DR   InterPro; IPR004501; PTS_IIC_lac.
DR   InterPro; IPR004801; PTS_IIC_lac_spec.
DR   Pfam; PF02378; PTS_EIIC; 1.
DR   Pfam; PF02302; PTS_IIB; 1.
DR   TIGRFAMs; TIGR00394; lac_pts_IIC; 1.
DR   TIGRFAMs; TIGR00410; lacE; 1.
DR   TIGRFAMs; TIGR00853; pts-lac; 1.
DR   PROSITE; PS51100; PTS_EIIB_TYPE_3; 1.
DR   PROSITE; PS51105; PTS_EIIC_TYPE_3; 1.
PE   4: Predicted;
KW   Cell membrane; Complete proteome; Kinase; Membrane;
KW   Phosphotransferase system; Sugar transport; Transferase;
KW   Transmembrane; Transport.
FT   CHAIN         1    570       PTS system lactose-specific EIICB
FT                                component.
FT                                /FTId=PRO_0000186589.
FT   TRANSMEM     30     50       Potential.
FT   TRANSMEM     61     81       Potential.
FT   TRANSMEM    102    122       Potential.
FT   TRANSMEM    131    151       Potential.
FT   TRANSMEM    174    194       Potential.
FT   TRANSMEM    219    239       Potential.
FT   TRANSMEM    278    298       Potential.
FT   TRANSMEM    334    354       Potential.
FT   TRANSMEM    375    395       Potential.
FT   DOMAIN        1    410       PTS EIIC type-3.
FT   DOMAIN      467    570       PTS EIIB type-3.
FT   ACT_SITE    474    474       Phosphocysteine intermediate; for EIIB
FT                                activity (By similarity).
SQ   SEQUENCE   570 AA;  62432 MW;  587192287079BE58 CRC64;
     MMQKLIAQIE KGKPFFEKLS RNIYLRAIRD GFISAMPVIL FSSIFLLIAY VPNIFGFKWD
     KGMEAILMKP YNYTMGLVAF LVAGTTAKSL TDSFNRKLES TNQINFISTM LAAMCGFLFL
     ASDPAKDGGF LSAFMGTKGL LTAFLSAFVT VIVYNFCVKR NITIKMPKEV PPNISQVFKD
     LIPFSAVIII LYALDLVIRN SFKSNVAEGI LKLFEPLFTA ADGWIGVTII FGAFALFWFV
     GIHGPSIVEP AIAAITYANI EANFKLLQAG EHADKIITSG TQMFIVTFGG TGATLVVPFM
     FMWMTKSKRN KAIGRASVVP TFFGVNEPIL FGAPLVLNPV FFIPFVLAPI VNVWIFKLFV
     EVLGMNSFSV NLPWTTPGPL GIIMGTGFGL WSFVLAITLI VVDIIIYYPF LKVYDSEILD
     EEEGRKESNS DLKEKVAANF DTKKADSILA ASGVSDDAAK ASNITEQTNV LVLCAGGGTS
     GLLANALNKA AEEYHVPVKA AAGGYGAHMD IMKEYQLIIL APQVASNYED IKQDTDRLGI
     KLAKTQGAEY IKLTRDGQAA LDFVQQQFEN
//