Reviewed, UniProtKB/Swiss-Prot Q6G7C4 (PTLCB_STAAS)

Names and origin

Protein names

Recommended name:
    PTS system lactose-specific EIICB component
Alternative name(s):
    EIICB-Lac
      Short name=EII-Lac
Including the following 2 domains:
    1- Recommended name:
            Lactose permease IIC component
        Alternative name(s):
            PTS system lactose-specific EIIC component
    2- Recommended name:
            Lactose-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system lactose-specific EIIB component

Gene names

Name:	lacE
Ordered Locus Names:	SAS2091

Organism

Staphylococcus aureus (strain MSSA476) [Complete proteome] [HAMAP]

Taxonomic identifier

282459 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	570 AA.
Sequence status	Complete.
Protein existence	Predicted.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in lactose transport.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-3 domain. Contains 1 PTS EIIC type-3 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar binding Inferred from electronic annotation. Source: InterPro sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 570	570	PTS system lactose-specific EIICB component		PRO_0000186589
Regions
	Transmembrane	30 – 50	21	Potential
	Transmembrane	61 – 81	21	Potential
	Transmembrane	102 – 122	21	Potential
	Transmembrane	131 – 151	21	Potential
	Transmembrane	174 – 194	21	Potential
	Transmembrane	219 – 239	21	Potential
	Transmembrane	278 – 298	21	Potential
	Transmembrane	334 – 354	21	Potential
	Transmembrane	375 – 395	21	Potential
	Domain	1 – 410	410	PTS EIIC type-3
	Domain	467 – 570	104	PTS EIIB type-3
Sites
	Active site	474	1	Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6G7C4-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 587192287079BE58	FASTA	570	62,432
	10 20 30 40 50 60 MMQKLIAQIE KGKPFFEKLS RNIYLRAIRD GFISAMPVIL FSSIFLLIAY VPNIFGFKWD 70 80 90 100 110 120 KGMEAILMKP YNYTMGLVAF LVAGTTAKSL TDSFNRKLES TNQINFISTM LAAMCGFLFL 130 140 150 160 170 180 ASDPAKDGGF LSAFMGTKGL LTAFLSAFVT VIVYNFCVKR NITIKMPKEV PPNISQVFKD 190 200 210 220 230 240 LIPFSAVIII LYALDLVIRN SFKSNVAEGI LKLFEPLFTA ADGWIGVTII FGAFALFWFV 250 260 270 280 290 300 GIHGPSIVEP AIAAITYANI EANFKLLQAG EHADKIITSG TQMFIVTFGG TGATLVVPFM 310 320 330 340 350 360 FMWMTKSKRN KAIGRASVVP TFFGVNEPIL FGAPLVLNPV FFIPFVLAPI VNVWIFKLFV 370 380 390 400 410 420 EVLGMNSFSV NLPWTTPGPL GIIMGTGFGL WSFVLAITLI VVDIIIYYPF LKVYDSEILD 430 440 450 460 470 480 EEEGRKESNS DLKEKVAANF DTKKADSILA ASGVSDDAAK ASNITEQTNV LVLCAGGGTS 490 500 510 520 530 540 GLLANALNKA AEEYHVPVKA AAGGYGAHMD IMKEYQLIIL APQVASNYED IKQDTDRLGI 550 560 570 KLAKTQGAEY IKLTRDGQAA LDFVQQQFEN

References

[1]

"Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance." Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J., Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A., Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C., Clark L., Corton C. , Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z., Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N., Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M., Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004) [PubMed: 15213324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571857 Genomic DNA. Translation: CAG43899.1.
RefSeq	YP_044200.1.
3D structure databases
SMR	Q6G7C4. Positions 466-567.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6G7C4.
Genome annotation databases
GeneID	2863141.
GenomeReviews	Gene locus SAS2091 in contig BX571857_GR.
KEGG	sas:SAS2091.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1455.
HOGENOM	HBG337916.
OMA	WSKDIEN.
Enzyme and pathway databases
BioCyc	SAUR282459:SAS2091-MONOMER.
Family and domain databases
InterPro	IPR014350. PTrfase_system_EIIB_3_subgr. IPR003501. PTS_EIIB_2/3. IPR013012. PTS_EIIB_3. IPR003352. PTS_EIIC. IPR004501. PTS_IIC_lac. IPR004801. PTS_IIC_lac_spec. [Graphical view]
Pfam	PF02378. PTS_EIIC. 1 hit. PF02302. PTS_IIB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00394. lac_pts_IIC. 1 hit. TIGR00410. lacE. 1 hit. TIGR00853. pts-lac. 1 hit.
PROSITE	PS51100. PTS_EIIB_TYPE_3. 1 hit. PS51105. PTS_EIIC_TYPE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTLCB_STAAS

Accession

Primary (citable) accession number: Q6G7C4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	July 19, 2004
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)