Reviewed, UniProtKB/Swiss-Prot Q6G664 (BETA_STAAS)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	SAS2497

Organism

Staphylococcus aureus (strain MSSA476) [Complete proteome] [HAMAP]

Taxonomic identifier

282459 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	569 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity.
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor.
Cofactor	FAD By similarity.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1.
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular alcohol metabolic process Inferred from electronic annotation. Source: InterPro glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 569	569	Choline dehydrogenase		PRO_0000205599
Regions
	Nucleotide binding	9 – 38	30	FAD Probable
Sites
	Active site	475	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6G664-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 927251B0DB8CDEF4	FASTA	569	63,624
	10 20 30 40 50 60 MSNKNKSYDY VIIGGGSAGS VLGNRLSEDK DKEVLVLEAG RSDYFWDLFI QMPAALMFPS 70 80 90 100 110 120 GNKFYDWIYS TDEEPHMGGR KVAHARGKVL GGSSSINGMI YQRGNPMDYE GWAEPEGMET 130 140 150 160 170 180 WDFAHCLPYF KKLEKTYGAA PYDKFRGHDG PIKLKRGPAT NPLFQSFFDA GVEAGYHKTP 190 200 210 220 230 240 DVNGFRQEGF GPFDSQVHRG RRMSASRAYL HPAMKRKNLT VETRAFVTEI HYEGRRATGV 250 260 270 280 290 300 TYKKNGKLHT IDAKEVILSG GAFNTPQLLQ LSGIGDSEFL KSKGIEPRVH LPGVGENFED 310 320 330 340 350 360 HLEVYIQHKC KEPVSLQPSL DIKRMPFIGL QWIFTRTGAA ASNHFEGGGF VRSNNEVDYP 370 380 390 400 410 420 NLMFHFLPIA VRYDGQKAAV AHGYQVHVGP MYSNSRGSLK IKSKDPFEKP SIRFNYLSTE 430 440 450 460 470 480 EDKKEWVEAI RVARNILSQK AMDPFNGGEI SPGPEVQTDE EILDWVRRDG ETALHPSCSA 490 500 510 520 530 540 KMGPASDPMA VVDPLTMKVH GMENLRVVDA SAMPRTTNGN IHAPVLMLAE KAADIIRGRK 550 560 PLEPQYIDYY KHGVHDENEG AIEVKPYAK

References

[1]

"Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance." Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J., Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A., Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C., Clark L., Corton C. , Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z., Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N., Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M., Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J. Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004) [PubMed: 15213324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX571857 Genomic DNA. Translation: CAG44313.1.
RefSeq	YP_044610.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2864237.
GenomeReviews	Gene locus SAS2497 in contig BX571857_GR.
KEGG	sas:SAS2497.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6G664.
Enzyme and pathway databases
BioCyc	SAUR282459:SAS2497-MON.
Family and domain databases
HAMAP	MF_00750. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_STAAS

Accession

Primary (citable) accession number: Q6G664

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	July 19, 2004
Last modified:	November 25, 2008
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)