Q6G543 (ILVD_BARHE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	BH00980

Organism

Bartonella henselae (strain ATCC 49882 / Houston 1) (Rochalimaea henselae) [Complete proteome] [HAMAP]

Taxonomic identifier

283166 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	612 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 612	612	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000225373
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6G543 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 51929D20E9C38E3A	FASTA	612	65,968
	10 20 30 40 50 60 MPSYRSRIST HGRNMAGARS LWRATGMKES DFGKPIIAIA NSFTQFVPGH VHLKDLGQLV 70 80 90 100 110 120 AQQIVASGGI AKEFNTIAID DGIAMGHDGM LYSLPSREII ADSVEYMINA HCADALVCIS 130 140 150 160 170 180 NCDKITPGML MASLRLNIPT IFVSGGPMEA GKIKWKDQDL TVDLVDAMVA AAAENNSEEE 190 200 210 220 230 240 VAEMERAACP TCGSCSGMFT ANSMNCLTEA LGLSLPGNGS MLATHADRQM LFEEAGRQIV 250 260 270 280 290 300 TLVKRYYEKG DETVLPRSIA SRKAFENAMT VDISMGGSTN TVLHLLAAAQ EGEVDFTMTE 310 320 330 340 350 360 IDRLSRRVPV LCKVAPAVAN VHMEDVHRAG GIMGLLGELD AVGLIDTSAY TVHAKTMKEA 370 380 390 400 410 420 LCRWDVKRTN EPKTHEFYRA APGGISTQTA FSQSRRYDSL DLDREKGIIR DKEHAYSQDG 430 440 450 460 470 480 GLAVLYGNLA KDGCIVKTAG VDQSILTFKG PARIFESQDS AVSAILNDTI QSGDIVLIRY 490 500 510 520 530 540 EGPRGGPGMQ EMLYPTSYLK SKGLGKVCAL ITDGRFSGGT SGLSIGHVSP EAAEGGAIAL 550 560 570 580 590 600 VEEGDIIEID IPNRTIHMLV EDDELMHRRV KMEAKGKAAW QPTEKRKRKV SKALKAYAAM 610 TTSAAKGAVR NI

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49882 / Houston 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX897699 Genomic DNA. Translation: CAF26913.1.
RefSeq	YP_032960.1. NC_005956.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2865557.
GenomeReviews	Gene locus BH00980 in contig BX897699_GR.
KEGG	bhe:BH00980.
NMPDR	fig|283166.1.peg.88.
PATRIC	20543909. VBIBarHen29080_0114.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	SQDCRWP.
PhylomeDB	Q6G543.
ProtClustDB	PRK12448.
Enzyme and pathway databases
BioCyc	BHEN283166:BH00980-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_BARHE

Accession

Primary (citable) accession number: Q6G543

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program