Q6G4Z1 (PROB_BARHE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate 5-kinase
EC=2.7.2.11

Alternative name(s):
Gamma-glutamyl kinase
Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	BH01570

Organism

Bartonella henselae (strain ATCC 49882 / Houston 1) (Rochalimaea henselae) [Complete proteome] [HAMAP]

Taxonomic identifier

283166 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella › Bartonella henselae

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00456.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	Glutamate 5-kinase HAMAP-Rule MF_00456		PRO_0000109644
Regions
	Domain	282 – 359	78	PUA
	Nucleotide binding	176 – 177	2	ATP By similarity
Sites
	Binding site	17	1	ATP By similarity
	Binding site	57	1	Substrate By similarity
	Binding site	144	1	Substrate By similarity
	Binding site	156	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6G4Z1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 0FA2C8C521CC0A7C	FASTA	379	40,845
	10 20 30 40 50 60 MAVGLQKLVH YKRIVIKIGS ALLVDPQTGL RAEWLKSLIS DVANLHQKGV EILLVSSGAI 70 80 90 100 110 120 ALGRTLLRLP KGALKLEESQ ACAALGQIEL AKTYSDTLAQ YGLKTGQILL TLFDTEERRR 130 140 150 160 170 180 YLNARATINV LLRFGAVPVI NENDTVATSE IRYGDNDRLA ARVATMMGAD LLILLSDIDG 190 200 210 220 230 240 LYTKSPHRDP TAEFIPFIAS ITSDIEKMAD VAHSELSRGG MKTKLDAGKI ANSAGTAMII 250 260 270 280 290 300 TSGKRMNPLA AIDRGERGSF FAAGEKPVNA WKTWISGHLD PSGILMIDQG AVKALESGKS 310 320 330 340 350 360 LLAAGVVAIE GRFNRGDTVA IVDTNGVEIA RGLVSYGKDE SVRIMGRKSE EIESILGYEA 370 RSAMVHRNDM VLRCLTDSA

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49882 / Houston 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX897699 Genomic DNA. Translation: CAF26969.1.
RefSeq	YP_033012.1. NC_005956.1.
3D structure databases
ProteinModelPortal	Q6G4Z1.
ModBase	Search...
Protein-protein interaction databases
STRING	283166.BH01570.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAF26969; CAF26969; BH01570.
GeneID	2865531.
KEGG	bhe:BH01570.
PATRIC	20544055. VBIBarHen29080_0184.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0263.
HOGENOM	HOG000246368.
KO	K00931.
OMA	DHLQLRG.
ProtClustDB	PRK05429.
Enzyme and pathway databases
BioCyc	BHEN283166:GIVZ-157-MONOMER.
UniPathway	UPA00098; UER00359.
Family and domain databases
Gene3D	2.30.130.10. 1 hit. 3.40.1160.10. 1 hit.
HAMAP	MF_00456. ProB.
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR019797. Glutamate_5-kinase_CS. IPR002478. PUA. IPR015947. PUA-like_domain. [Graphical view]
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF88697. PUA-like. 1 hit.
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_BARHE

Accession

Primary (citable) accession number: Q6G4Z1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	July 19, 2004
Last modified:	May 29, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program