Reviewed, UniProtKB/Swiss-Prot Q6G4Z1 (PROB_BARHE)

Names and origin

Protein names

Recommended name:
    Glutamate 5-kinase
    EC=2.7.2.11
Alternative name(s):
    Gamma-glutamyl kinase
      Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	BH01570

Organism

Bartonella henselae (Rochalimaea henselae) [Complete proteome] [HAMAP]

Taxonomic identifier

38323 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	Glutamate 5-kinase HAMAP MF_00456		PRO_0000109644
Regions
	Domain	282 – 359	78	PUA

Sequences

	Sequence		Length	Mass (Da)
	Q6G4Z1-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 0FA2C8C521CC0A7C	FASTA	379	40,845
	10 20 30 40 50 60 MAVGLQKLVH YKRIVIKIGS ALLVDPQTGL RAEWLKSLIS DVANLHQKGV EILLVSSGAI 70 80 90 100 110 120 ALGRTLLRLP KGALKLEESQ ACAALGQIEL AKTYSDTLAQ YGLKTGQILL TLFDTEERRR 130 140 150 160 170 180 YLNARATINV LLRFGAVPVI NENDTVATSE IRYGDNDRLA ARVATMMGAD LLILLSDIDG 190 200 210 220 230 240 LYTKSPHRDP TAEFIPFIAS ITSDIEKMAD VAHSELSRGG MKTKLDAGKI ANSAGTAMII 250 260 270 280 290 300 TSGKRMNPLA AIDRGERGSF FAAGEKPVNA WKTWISGHLD PSGILMIDQG AVKALESGKS 310 320 330 340 350 360 LLAAGVVAIE GRFNRGDTVA IVDTNGVEIA RGLVSYGKDE SVRIMGRKSE EIESILGYEA 370 RSAMVHRNDM VLRCLTDSA

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49882 / Houston 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX897699 Genomic DNA. Translation: CAF26969.1.
RefSeq	YP_033012.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2865531.
GenomeReviews	Gene locus BH01570 in contig BX897699_GR.
KEGG	bhe:BH01570.
NMPDR	fig|283166.1.peg.140.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6G4Z1.
OMA	ILTDQRG.
Enzyme and pathway databases
BioCyc	BHEN283166:BH01570-MON.
BRENDA	2.7.2.11. 277357.
Family and domain databases
HAMAP	MF_00456. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu_5kinase. IPR011529. Glu_5kinase_bact. IPR019797. Glutamate_5-kinase_CS. IPR005715. ProB. IPR002478. PUA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_BARHE

Accession

Primary (citable) accession number: Q6G4Z1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	July 19, 2004
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)