Reviewed, UniProtKB/Swiss-Prot Q6G4W3 (TRMB_BARHE)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase
    EC=2.1.1.33
Alternative name(s):
    tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	BH02190

Organism

Bartonella henselae (Rochalimaea henselae) [Complete proteome] [HAMAP]

Taxonomic identifier

38323 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	233 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(7)-methylguanine. HAMAP MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP MF_01057
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Molecular function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 233	233	tRNA (guanine-N(7)-)-methyltransferase HAMAP MF_01057		PRO_0000171297
Regions
	Region	212 – 215	4	Substrate binding Potential
Sites
	Binding site	62	1	S-adenosyl-L-methionine By similarity
	Binding site	87	1	S-adenosyl-L-methionine By similarity
	Binding site	116	1	S-adenosyl-L-methionine By similarity
	Binding site	138	1	S-adenosyl-L-methionine By similarity
	Binding site	142	1	Substrate By similarity
	Binding site	174	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6G4W3-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 778154B3E04E820F	FASTA	233	27,515
	10 20 30 40 50 60 MIDHNIRTGK AFFGRRKGKR LRNSQLVLIK KLFPTLDINL NNSVPLNLTS LFSRKVKEVR 70 80 90 100 110 120 LEIGFGGGEH LLHEMKHFPQ TGFIGIEPFI NGMAKMLMSL EEHKQYQNQL RLYNDDATGL 130 140 150 160 170 180 LDWLPNASLD GIDLFYPDPW PKKKHWKRRF INMKNLNRFA RVLKTGKKFR FASDIESYVN 190 200 210 220 230 WTLYHCSNHH SFEWEAKNPK DWKTPYTLWS GTRYEAKALR EGRAPTYLTF IKK

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49882 / Houston 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX897699 Genomic DNA. Translation: CAF27031.1.
RefSeq	YP_033072.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2864622.
GenomeReviews	Gene locus BH02190 in contig BX897699_GR.
KEGG	bhe:BH02190.
NMPDR	fig|283166.1.peg.200.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6G4W3.
OMA	Q6G4W3. YPDPWPK.
Enzyme and pathway databases
BioCyc	BHEN283166:BH02190-MON.
BRENDA	2.1.1.33. 277357.
Family and domain databases
HAMAP	MF_01057. [Tree]
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
PANTHER	PTHR23417:SF1. Methyltransf_4. 1 hit.
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRMB_BARHE

Accession

Primary (citable) accession number: Q6G4W3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	July 19, 2004
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)