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Q6G4S5

- SYI_BARHE

UniProt

Q6G4S5 - SYI_BARHE

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Bartonella henselae (strain ATCC 49882 / Houston 1) (Rochalimaea henselae)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (19 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei602 – 6021Aminoacyl-adenylateUniRule annotation
    Binding sitei646 – 6461ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBHEN283166:GIVZ-258-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:BH02580
    OrganismiBartonella henselae (strain ATCC 49882 / Houston 1) (Rochalimaea henselae)
    Taxonomic identifieri283166 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBartonellaceaeBartonella
    ProteomesiUP000000421: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 971971Isoleucine--tRNA ligasePRO_0000098353Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi283166.BH02580.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6G4S5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi64 – 7411"HIGH" regionAdd
    BLAST
    Motifi643 – 6475"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6G4S5-1 [UniParc]FASTAAdd to Basket

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    MTVKNETVDY SKTLYLPQTN FPMRAGLPQK ELELMERWEK RGLYAQLRQQ    50
    AKDRPLYTLH DGPPYANGHI HIGHALNKVL KDVIIRSFQM RGFNANYVPG 100
    WDCHGLPIEW KIEEKYRAQG KNKDDVPLNE FRQECRQFAQ HWITVQSEEF 150
    KRLGVVGDFN RPYTTMAFHA EARIASELMK FALSDQIYRG SKPVMWSVVE 200
    RTALAEAEIE YHDHESDVIW VKFPVLQADS KDLYDAYVVI WTTTPWTIPA 250
    NRAVSYSSQI SYSIYEVKSA ENDFGPQVGE KLLFADALVM SCAEKAKLVL 300
    KRLRVISAKE FKTLVLSHPL KGLAGGYNNK IAMLDGSHVT ESAGTGFVHT 350
    APSHGREDFE IWNAYKPLLE QSGIDSSIPF PVDDAGFYTK DAPGFGPDRK 400
    GGAIRVIDDN GKMGDANKEV INALIKADRL FARGRLKHSY PHSWRSKKPI 450
    IFRNTPQWFI SMDKDLGDGS TLRSRALKAI SMTRFVPSSG QNRLASMIAD 500
    RPDWVLSRQR AWGVPICIFA NEDGVILKDE RVNERILRAF EAEGADAWFA 550
    EGARERFLGE RAHESWIQVV DILDVWFDSG ASHSFVLEDR DDLNWPADVY 600
    FEGSDQHRGW FQSSLLESCG TRACSPYKAV ITHGFTLDEN GKKMSKSLGN 650
    TVVPQEIIKT FGADIFRLWV MTTDYWEDQR LGKQILQTNV DSYRKLRNAI 700
    RWMLGTLAHD EGEEISYCAL PDLEKLILHR LSELDQLVNR AYDDFDFKKI 750
    MRALLDFSIT ELSAFYFDIR KDSLYCDPPS SKKRKASLQV VREIFERMVI 800
    WLAPMLPFTM EEAWLERYPE STSVHLEQFR PVPMEWQNES LAERWKKIRQ 850
    VRKVVTGALE LERADKRIGS SLEAAPIVFI SNPVLREALE NLDMAEICIT 900
    SALTITQGVP PSDAFILSDV EGVGVYPRKA LGTKCARSWR YTQDVGSDPT 950
    YPDVSARDAA ALRELQVLGK I 971
    Length:971
    Mass (Da):110,734
    Last modified:July 19, 2004 - v1
    Checksum:iD404145683BF5EDA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX897699 Genomic DNA. Translation: CAF27070.1.
    RefSeqiYP_033110.1. NC_005956.1.

    Genome annotation databases

    EnsemblBacteriaiCAF27070; CAF27070; BH02580.
    GeneIDi2865553.
    KEGGibhe:BH02580.
    PATRICi20544261. VBIBarHen29080_0287.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX897699 Genomic DNA. Translation: CAF27070.1 .
    RefSeqi YP_033110.1. NC_005956.1.

    3D structure databases

    ProteinModelPortali Q6G4S5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 283166.BH02580.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAF27070 ; CAF27070 ; BH02580 .
    GeneIDi 2865553.
    KEGGi bhe:BH02580.
    PATRICi 20544261. VBIBarHen29080_0287.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci BHEN283166:GIVZ-258-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 49882 / Houston 1.

    Entry informationi

    Entry nameiSYI_BARHE
    AccessioniPrimary (citable) accession number: Q6G4S5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: July 19, 2004
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3