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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Bartonella henselae (strain ATCC 49882 / DSM 28221 / Houston 1) (Rochalimaea henselae)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei602Aminoacyl-adenylateUniRule annotation1
Binding sitei646ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBHEN283166:G1GJ7-265-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BH02580
OrganismiBartonella henselae (strain ATCC 49882 / DSM 28221 / Houston 1) (Rochalimaea henselae)
Taxonomic identifieri283166 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBartonellaceaeBartonella
Proteomesi
  • UP000000421 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000983531 – 971Isoleucine--tRNA ligaseAdd BLAST971

Proteomic databases

PaxDbiQ6G4S5
PRIDEiQ6G4S5

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi283166.BH02580

Structurei

3D structure databases

ProteinModelPortaliQ6G4S5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi64 – 74"HIGH" regionAdd BLAST11
Motifi643 – 647"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.6202 hits
3.90.740.101 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q6G4S5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVKNETVDY SKTLYLPQTN FPMRAGLPQK ELELMERWEK RGLYAQLRQQ
60 70 80 90 100
AKDRPLYTLH DGPPYANGHI HIGHALNKVL KDVIIRSFQM RGFNANYVPG
110 120 130 140 150
WDCHGLPIEW KIEEKYRAQG KNKDDVPLNE FRQECRQFAQ HWITVQSEEF
160 170 180 190 200
KRLGVVGDFN RPYTTMAFHA EARIASELMK FALSDQIYRG SKPVMWSVVE
210 220 230 240 250
RTALAEAEIE YHDHESDVIW VKFPVLQADS KDLYDAYVVI WTTTPWTIPA
260 270 280 290 300
NRAVSYSSQI SYSIYEVKSA ENDFGPQVGE KLLFADALVM SCAEKAKLVL
310 320 330 340 350
KRLRVISAKE FKTLVLSHPL KGLAGGYNNK IAMLDGSHVT ESAGTGFVHT
360 370 380 390 400
APSHGREDFE IWNAYKPLLE QSGIDSSIPF PVDDAGFYTK DAPGFGPDRK
410 420 430 440 450
GGAIRVIDDN GKMGDANKEV INALIKADRL FARGRLKHSY PHSWRSKKPI
460 470 480 490 500
IFRNTPQWFI SMDKDLGDGS TLRSRALKAI SMTRFVPSSG QNRLASMIAD
510 520 530 540 550
RPDWVLSRQR AWGVPICIFA NEDGVILKDE RVNERILRAF EAEGADAWFA
560 570 580 590 600
EGARERFLGE RAHESWIQVV DILDVWFDSG ASHSFVLEDR DDLNWPADVY
610 620 630 640 650
FEGSDQHRGW FQSSLLESCG TRACSPYKAV ITHGFTLDEN GKKMSKSLGN
660 670 680 690 700
TVVPQEIIKT FGADIFRLWV MTTDYWEDQR LGKQILQTNV DSYRKLRNAI
710 720 730 740 750
RWMLGTLAHD EGEEISYCAL PDLEKLILHR LSELDQLVNR AYDDFDFKKI
760 770 780 790 800
MRALLDFSIT ELSAFYFDIR KDSLYCDPPS SKKRKASLQV VREIFERMVI
810 820 830 840 850
WLAPMLPFTM EEAWLERYPE STSVHLEQFR PVPMEWQNES LAERWKKIRQ
860 870 880 890 900
VRKVVTGALE LERADKRIGS SLEAAPIVFI SNPVLREALE NLDMAEICIT
910 920 930 940 950
SALTITQGVP PSDAFILSDV EGVGVYPRKA LGTKCARSWR YTQDVGSDPT
960 970
YPDVSARDAA ALRELQVLGK I
Length:971
Mass (Da):110,734
Last modified:July 19, 2004 - v1
Checksum:iD404145683BF5EDA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX897699 Genomic DNA Translation: CAF27070.1
RefSeqiWP_011180208.1, NZ_LRIJ01000002.1

Genome annotation databases

EnsemblBacteriaiCAF27070; CAF27070; BH02580
GeneIDi29620763
KEGGibhe:BH02580

Similar proteinsi

Entry informationi

Entry nameiSYI_BARHE
AccessioniPrimary (citable) accession number: Q6G4S5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 19, 2004
Last modified: March 28, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome