Reviewed, UniProtKB/Swiss-Prot Q6G4S5 (SYI_BARHE)

Names and origin

Protein names

Recommended name:
    Isoleucyl-tRNA synthetase
    EC=6.1.1.5
Alternative name(s):
    Isoleucine--tRNA ligase
      Short name=IleRS

Gene names

Name:	ileS
Ordered Locus Names:	BH02580

Organism

Bartonella henselae (Rochalimaea henselae) [Complete proteome] [HAMAP]

Taxonomic identifier

38323 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	971 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP MF_02002
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02002
Subunit structure	Monomer By similarity. HAMAP MF_02002
Subcellular location	Cytoplasm By similarity HAMAP MF_02002.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP MF_02002
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 971	971	Isoleucyl-tRNA synthetase HAMAP MF_02002		PRO_0000098353
Regions
	Motif	64 – 74	11	"HIGH" region HAMAP MF_02002
	Motif	643 – 647	5	"KMSKS" region HAMAP MF_02002
Sites
	Binding site	602	1	Aminoacyl-adenylate By similarity
	Binding site	646	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6G4S5-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: D404145683BF5EDA	FASTA	971	110,734
	10 20 30 40 50 60 MTVKNETVDY SKTLYLPQTN FPMRAGLPQK ELELMERWEK RGLYAQLRQQ AKDRPLYTLH 70 80 90 100 110 120 DGPPYANGHI HIGHALNKVL KDVIIRSFQM RGFNANYVPG WDCHGLPIEW KIEEKYRAQG 130 140 150 160 170 180 KNKDDVPLNE FRQECRQFAQ HWITVQSEEF KRLGVVGDFN RPYTTMAFHA EARIASELMK 190 200 210 220 230 240 FALSDQIYRG SKPVMWSVVE RTALAEAEIE YHDHESDVIW VKFPVLQADS KDLYDAYVVI 250 260 270 280 290 300 WTTTPWTIPA NRAVSYSSQI SYSIYEVKSA ENDFGPQVGE KLLFADALVM SCAEKAKLVL 310 320 330 340 350 360 KRLRVISAKE FKTLVLSHPL KGLAGGYNNK IAMLDGSHVT ESAGTGFVHT APSHGREDFE 370 380 390 400 410 420 IWNAYKPLLE QSGIDSSIPF PVDDAGFYTK DAPGFGPDRK GGAIRVIDDN GKMGDANKEV 430 440 450 460 470 480 INALIKADRL FARGRLKHSY PHSWRSKKPI IFRNTPQWFI SMDKDLGDGS TLRSRALKAI 490 500 510 520 530 540 SMTRFVPSSG QNRLASMIAD RPDWVLSRQR AWGVPICIFA NEDGVILKDE RVNERILRAF 550 560 570 580 590 600 EAEGADAWFA EGARERFLGE RAHESWIQVV DILDVWFDSG ASHSFVLEDR DDLNWPADVY 610 620 630 640 650 660 FEGSDQHRGW FQSSLLESCG TRACSPYKAV ITHGFTLDEN GKKMSKSLGN TVVPQEIIKT 670 680 690 700 710 720 FGADIFRLWV MTTDYWEDQR LGKQILQTNV DSYRKLRNAI RWMLGTLAHD EGEEISYCAL 730 740 750 760 770 780 PDLEKLILHR LSELDQLVNR AYDDFDFKKI MRALLDFSIT ELSAFYFDIR KDSLYCDPPS 790 800 810 820 830 840 SKKRKASLQV VREIFERMVI WLAPMLPFTM EEAWLERYPE STSVHLEQFR PVPMEWQNES 850 860 870 880 890 900 LAERWKKIRQ VRKVVTGALE LERADKRIGS SLEAAPIVFI SNPVLREALE NLDMAEICIT 910 920 930 940 950 960 SALTITQGVP PSDAFILSDV EGVGVYPRKA LGTKCARSWR YTQDVGSDPT YPDVSARDAA 970 ALRELQVLGK I

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49882 / Houston 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX897699 Genomic DNA. Translation: CAF27070.1.
RefSeq	YP_033110.1.
3D structure databases
SMR	Q6G4S5. Positions 8-965.
ModBase	Search...
Genome annotation databases
GeneID	2865553.
GenomeReviews	Gene locus BH02580 in contig BX897699_GR.
KEGG	bhe:BH02580.
NMPDR	fig|283166.1.peg.238.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG577712.
OMA	KQVLTHG.
PhylomeDB	Q6G4S5.
Enzyme and pathway databases
BioCyc	BHEN283166:BH02580-MONOMER.
BRENDA	6.1.1.5. 277357.
Family and domain databases
HAMAP	MF_02002. Ile_tRNA_synth_type1. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-synt_Ia. IPR015905. Ile-tRNA-synt_Ia_N. IPR018353. Isoleucyl-tRNA_synthetase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_Ia_edit. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF9. Ile-tRNA-synt_Ia. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_BARHE

Accession

Primary (citable) accession number: Q6G4S5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	July 19, 2004
Last modified:	February 9, 2010
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)