Reviewed, UniProtKB/Swiss-Prot Q6G4G5 (PURA_BARHE)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate synthetase
    EC=6.3.4.4
Alternative name(s):
    IMP--aspartate ligase
    AdSS
    AMPSase

Gene names

Name:	purA
Ordered Locus Names:	BH03790

Organism

Bartonella henselae (Rochalimaea henselae) [Complete proteome] [HAMAP]

Taxonomic identifier

38323 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP adenylosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	Adenylosuccinate synthetase HAMAP MF_00011		PRO_0000224257
Regions
	Nucleotide binding	12 – 18	7	GTP Potential
Sites
	Active site	140	1	By similarity
	Active site	147	1	By similarity
	Metal binding	13	1	Magnesium By similarity
	Metal binding	40	1	Magnesium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6G4G5-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 20FC75928931B541	FASTA	429	47,190
	10 20 30 40 50 60 MANVVVVGTQ WGDEGKGKIV DWLSERADII VRYQGGHNAG HTLVVNGVNY KLSLLPSGLV 70 80 90 100 110 120 RGKLSIIGNG VVVDPHHFVV ELKKLRDQGV KIVPEILRIA ENAPLILSLH RDLDAIRESG 130 140 150 160 170 180 FSGLKIGTTK RGIGPAYEDK VGRRAIRVMD LAEANTLMAK IKRLLRHHNA LRRGMGVAEI 190 200 210 220 230 240 DSQALYDELM QVADEILPFM DCTWRLLDES YRMGKHVLFE GAQGALLDND FGTYPYVTSS 250 260 270 280 290 300 NTVAGQACTG SGMGPGAIHY VLGIAKAYTT RVGEGPFPTE QINDIGEFLG IRGHEFGVVT 310 320 330 340 350 360 GRKRRCGWFD AVLVRQMVTI CGVQGIALTK LDVLDGLDEI KVCVGYELDG KRIDYLPSSM 370 380 390 400 410 420 GAQSRVKPIY ETLEGWKQAT AHALKWEDLP VQAIKYIRYI EELIGTQVAL LSTSPEREDT ILITDPFAN

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49882 / Houston 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX897699 Genomic DNA. Translation: CAF27189.1.
RefSeq	YP_033220.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2864766.
GenomeReviews	Gene locus BH03790 in contig BX897699_GR.
KEGG	bhe:BH03790.
NMPDR	fig|283166.1.peg.348.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6G4G5.
OMA	ERDDTIV.
Enzyme and pathway databases
BioCyc	BHEN283166:BH03790-MON.
BRENDA	6.3.4.4. 277357.
Family and domain databases
HAMAP	MF_00011. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
ProDom	PD001188. Asucc_synthtase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. purA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA_BARHE

Accession

Primary (citable) accession number: Q6G4G5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	July 19, 2004
Last modified:	November 3, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)