Reviewed, UniProtKB/Swiss-Prot Q6G4F7 (PYRD_BARHE)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.3.1
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	BH03870

Organism

Bartonella henselae (Rochalimaea henselae) [Complete proteome] [HAMAP]

Taxonomic identifier

38323 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + O2 = orotate + H2O2. HAMAP MF_00225
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6. HAMAP MF_00225
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	Dihydroorotate dehydrogenase HAMAP MF_00225		PRO_0000336454
Sites
	Active site	171	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6G4F7-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 52B31703E536D0B4	FASTA	362	40,555
	10 20 30 40 50 60 MSFFRCIGRS ALFMLDPEHA HRLAIMGLKS GLNSYQKVVD NRLCVTIAGL KFENFIGLAA 70 80 90 100 110 120 GFDKNAEVVN DVFHLGFGFT EIGTVTPRPQ VGNPKPRLFR LRKDEAIINR MGFNNDGRQI 130 140 150 160 170 180 VYGRLHGYKR LGIVGINIGA NKDTVDKIDD YITSIAYFYD VADYFTVNIS SPNTPGLRDL 190 200 210 220 230 240 QVRDSLHLLM NAISQARNEQ KKKHGFFVPI FLKIAPDLSE KELDDVAEEM KLSDFDGLIV 250 260 270 280 290 300 SNTTLSRQGL RECTLRNEEG GLSGRPLFER STIVLAKMRQ KLGKKIAIIG VGGIRDAKTA 310 320 330 340 350 360 LEKVKAGADL VQLYSGMVYE GPDLAITILK EILQFMQKDG VESIKAYRDQ RVEYWAKHML SS

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49882 / Houston 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX897699 Genomic DNA. Translation: CAF27197.1.
RefSeq	YP_033228.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2864782.
GenomeReviews	Gene locus BH03870 in contig BX897699_GR.
KEGG	bhe:BH03870.
NMPDR	fig|283166.1.peg.356.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6G4F7.
OMA	Q6G4F7. NASTELI.
Enzyme and pathway databases
BioCyc	BHEN283166:BH03870-MON.
Family and domain databases
HAMAP	MF_00225. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005719. Dihydroorotate_DH_2. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_BARHE

Accession

Primary (citable) accession number: Q6G4F7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	July 19, 2004
Last modified:	June 16, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)