Q6G4D1 (DXS_BARHE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	BH04350

Organism

Bartonella henselae (strain ATCC 49882 / Houston 1) (Rochalimaea henselae) [Complete proteome] [HAMAP]

Taxonomic identifier

283166 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	640 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 640	640	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_0000256380

Sequences

	Sequence		Length	Mass (Da)
	Q6G4D1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 938C1123AB45BA97	FASTA	640	69,918
	10 20 30 40 50 60 MSRTLTPLLD RVCEPQDLRA FPESDLARLA DELRAETIDV VSVTGGHLGA GLGVIELTVA 70 80 90 100 110 120 LHYVFNTPED RIIWDVGHQA YPHKILTGRR EKIRTLRQEG GLSGFTKRSE SVYDPFGAGH 130 140 150 160 170 180 SSTSISAGLG MAVASALKAE KRRNIVAVIG DGAMSAGMAY EAMNNAGALN ARLIVVLNDN 190 200 210 220 230 240 DMSIAPPTGA MSAHLARLVS RPAYRSLRER VKVLGEKLPK FFLDKARRSE EFARGFLVGG 250 260 270 280 290 300 TLFEELGFYY VGPIDGHNLK HLLPVLKNVR EYPTGPVLIH VVTHKGKGYA PAEASLDKYH 310 320 330 340 350 360 GVNRFDIITG KQVKASSNVL PYTKVFSKAL REEASYDDKI VAITAAMPTG TGLDFFAEKF 370 380 390 400 410 420 PERMFDVGIA EQHAVTFAAG MACEGYKPFV AIYSTFLQRA YDQIIHDVSI QKLPVRFAID 430 440 450 460 470 480 RAGFVGADGA THAGSFDIVF LTTLPEFVVM APSDEVELMH MVRTAAAYDQ GPISFRYPRG 490 500 510 520 530 540 EGIGMDLPQR GEILEIGKGR ILHEGNRIAL VCFGTRISEV LVAADELSAE GLSTTVADAR 550 560 570 580 590 600 FAKPLDKDLM RRLAREHEVL VTIEEGAIGG FGAHLLQFLA QEGLLEHGLK VRTLKLPDEY 610 620 630 640 LNHGSQEKVL SRIGLDAVGI VNTVFSALGR KIRTVQRVRV

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49882 / Houston 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX897699 Genomic DNA. Translation: CAF27244.1.
RefSeq	YP_033273.1. NC_005956.1.
3D structure databases
ProteinModelPortal	Q6G4D1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2865144.
GenomeReviews	Gene locus BH04350 in contig BX897699_GR.
KEGG	bhe:BH04350.
NMPDR	fig|283166.1.peg.401.
PATRIC	20544645. VBIBarHen29080_0476.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG571647.
OMA	DPILYHG.
PhylomeDB	Q6G4D1.
ProtClustDB	PRK05444.
Enzyme and pathway databases
BioCyc	BHEN283166:BH04350-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR001017. DH_E1. IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_BARHE

Accession

Primary (citable) accession number: Q6G4D1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program