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Protein

4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Gene

ispH

Organism
Bartonella henselae (strain ATCC 49882 / DSM 28221 / Houston 1) (Rochalimaea henselae)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.UniRule annotation

Catalytic activityi

Isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+.UniRule annotation
Dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: dimethylallyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway dimethylallyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate, the pathway dimethylallyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 6 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Bifunctional enzyme IspD/IspF (ispDF)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. Bifunctional enzyme IspD/IspF (ispDF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi18Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei47SubstrateUniRule annotation1
Binding sitei83SubstrateUniRule annotation1
Metal bindingi105Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei133SubstrateUniRule annotation1
Active sitei135Proton donorUniRule annotation1
Binding sitei174SubstrateUniRule annotation1
Metal bindingi204Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei277SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processIsoprene biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00056; UER00097.
UPA00059; UER00105.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductaseUniRule annotation (EC:1.17.7.4UniRule annotation)
Short name:
HMBPP reductaseUniRule annotation
Gene namesi
Name:ispHUniRule annotation
Synonyms:lytB
Ordered Locus Names:BH04410
OrganismiBartonella henselae (strain ATCC 49882 / DSM 28221 / Houston 1) (Rochalimaea henselae)
Taxonomic identifieri283166 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBartonellaceaeBartonella
Proteomesi
  • UP000000421 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001287781 – 3434-hydroxy-3-methylbut-2-enyl diphosphate reductaseAdd BLAST343

Proteomic databases

PaxDbiQ6G4C5.
PRIDEiQ6G4C5.

Interactioni

Protein-protein interaction databases

STRINGi283166.BH04410.

Structurei

3D structure databases

SMRiQ6G4C5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni232 – 234Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the IspH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C48. Bacteria.
COG0761. LUCA.
HOGENOMiHOG000220192.
KOiK03527.
OMAiTKVHKEA.
OrthoDBiPOG091H038O.

Family and domain databases

CDDicd13944. lytB_ispH. 1 hit.
HAMAPiMF_00191. IspH. 1 hit.
InterProiView protein in InterPro
IPR003451. LytB/IspH.
PANTHERiPTHR30426. PTHR30426. 1 hit.
PfamiView protein in Pfam
PF02401. LYTB. 1 hit.
TIGRFAMsiTIGR00216. ispH_lytB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6G4C5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSILSPLIIR LCNPRGFCAG VDRAIQIVLL ALKKYGAPVY VRHEIVHNRY
60 70 80 90 100
VVEGLQQRGA IFVEELDEIP EEHRNQPVVF SAHGVPKSVP EQADCYNLFY
110 120 130 140 150
LDATCPLVSK VHKQAMRHQR HRRHVILIGH AGHPEVIGTM GQLEKGGVTL
160 170 180 190 200
IETVEDALHY QPDDPDNLGF VTQTTLSVED TAEILDVLQR RFPALEPPAA
210 220 230 240 250
ESICYATTNR QNAVKAAALG SDLFLIVGAP NSSNSRRLVE VAERSGARQS
260 270 280 290 300
ILVQRADEID FDHLGALSVV SLSAGASAPE IIVDEIISAF RKRYNVTIEL
310 320 330 340
AETVVENETF LVNRELRDVV LTPQDMAFMN GRSEMLKNKN QDM
Length:343
Mass (Da):38,073
Last modified:July 19, 2004 - v1
Checksum:iF6A0CEBC6F8F7259
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX897699 Genomic DNA. Translation: CAF27250.1.
RefSeqiWP_011180374.1. NC_005956.1.

Genome annotation databases

EnsemblBacteriaiCAF27250; CAF27250; BH04410.
GeneIDi29621159.
KEGGibhe:BH04410.

Similar proteinsi

Entry informationi

Entry nameiISPH_BARHE
AccessioniPrimary (citable) accession number: Q6G4C5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2004
Last modified: October 25, 2017
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families