Reviewed, UniProtKB/Swiss-Prot Q6G463 (PYRE_BARHE)

Names and origin

Protein names

Recommended name:
    Orotate phosphoribosyltransferase
      Short name=OPRT
      Short name=OPRTase
    EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	BH05050

Organism

Bartonella henselae (Rochalimaea henselae) [Complete proteome] [HAMAP]

Taxonomic identifier

38323 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	192 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 192	192	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_1000066207
Regions
	Region	116 – 124	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	120	1	Orotate By similarity
	Binding site	148	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6G463-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 763114BC02F510A2	FASTA	192	20,869
	10 20 30 40 50 60 MNTQDVIDIF KQADAILEGH FILTSGRHSA TYMQKAKVFM HAHLTEKLCR GLAEKIKKSV 70 80 90 100 110 120 EEKIDYVVGP AIGGLIPSYE TSRHLGIPSL WVERVNGRFE LRRFEIKKGA RVVIVEDIVT 130 140 150 160 170 180 TGLSIRETVE ALVTAGANVL ASACILDRSG GKVDVGVPLI SLAEYEIASY ASDAVPAELA 190 ELPAIKPGSR NI

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49882 / Houston 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX897699 Genomic DNA. Translation: CAF27313.1.
RefSeq	YP_033341.1.
3D structure databases
HSSP	HSSP built from PDB template 1VDM based on UniProtKB O57827.
SMR	Q6G463. Positions 3-174.
ModBase	Search...
Genome annotation databases
GeneID	2864783.
GenomeReviews	Gene locus BH05050 in contig BX897699_GR.
KEGG	bhe:BH05050.
NMPDR	fig|283166.1.peg.469.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG404341.
OMA	FYLQSAK.
Enzyme and pathway databases
BioCyc	BHEN283166:BH05050-MONOMER.
BRENDA	2.4.2.10. 277357.
Family and domain databases
HAMAP	MF_01208_B. PyrE_B. [Tree]
InterPro	IPR006273. Orotate_PRibTrfase_thermus-typ. IPR000836. PRibTrfase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01367. pyrE_Therm. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_BARHE

Accession

Primary (citable) accession number: Q6G463

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	July 19, 2004
Last modified:	February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)