Reviewed, UniProtKB/Swiss-Prot Q6G3Z8 (ISPDF_BARHE)

Names and origin

Protein names

Recommended name:
    Bifunctional enzyme ispD/ispF
Including the following 2 domains:
    1- Recommended name:
            2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
              EC=2.7.7.60
        Alternative name(s):
            4-diphosphocytidyl-2C-methyl-D-erythritol synthase
            MEP cytidylyltransferase
              Short name=MCT
    2- Recommended name:
            2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
                Short name=MECPS
                Short name=MECDP-synthase
              EC=4.6.1.12

Gene names

Name:	ispDF
Ordered Locus Names:	BH05820

Organism

Bartonella henselae (Rochalimaea henselae) [Complete proteome] [HAMAP]

Taxonomic identifier

38323 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (ispD), and converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP (ispF) By similarity.
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_01520 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP. HAMAP MF_01520
Cofactor	Divalent metal cations By similarity.
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_01520 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.
Sequence similarities	In the N-terminal section; belongs to the ispD family. In the C-terminal section; belongs to the ispF family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding
Molecular function	Lyase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity Inferred from electronic annotation. Source: HAMAP 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 397	397	Bifunctional enzyme ispD/ispF HAMAP MF_01520		PRO_0000075656
Regions
	Region	1 – 236	236	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_01520
	Region	237 – 397	161	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase HAMAP MF_01520
Sites
	Metal binding	243	1	Divalent metal cation By similarity
	Metal binding	245	1	Divalent metal cation By similarity
	Metal binding	277	1	Divalent metal cation By similarity
	Site	15	1	Transition state stabilizer By similarity
	Site	24	1	Transition state stabilizer By similarity
	Site	155	1	Positions MEP for the nucleophilic attack By similarity
	Site	212	1	Positions MEP for the nucleophilic attack By similarity
	Site	269	1	Transition state stabilizer By similarity
	Site	368	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6G3Z8-1 [UniParc]. Last modified June 26, 2007. Version 2. Checksum: 1488BE631F41E982	FASTA	397	44,057
	10 20 30 40 50 60 MSIAAVILAA GRGKRAGSLP KKPKQYRLLG QEPVICHTVR CFCQNPAITT IILVIHPEDR 70 80 90 100 110 120 QICEQAIADF KEQLIIVEGG NTRQKSTLRG LQALRKFKPK YVHIHDGARP FVENKLLEQI 130 140 150 160 170 180 HTTVTPQEGV LPVLAVCDTL KRINSTHHVL ETIPRTHLYS AQTPQCFPFE RILAAHEKAI 190 200 210 220 230 240 KTCKKEFTDD SAIAEWFGIS MRTIPGSPHN IKITWHEDLN TAHLYLQKKM QMFPDIRTGN 250 260 270 280 290 300 GYDVHSFEEG NSLILCGIKI PFHKKLNGHS DADVALHALT DALLATRGAG DIGTHFPPSD 310 320 330 340 350 360 PQWQNVSSEI FLRHALDIVK QAGGRIANVD ITLIAEEPKI GPYRHAMVGN LMNMLTILPD 370 380 390 RISIKATTNE KLGFIGRGEG IAAFATANVL YPGEIPK

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49882 / Houston 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX897699 Genomic DNA. Translation: CAF27390.1. Different initiation.
3D structure databases
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus BH05820 in contig BX897699_GR.
KEGG	bhe:BH05820.
NMPDR	fig|283166.1.peg.544.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6G3Z8.
Enzyme and pathway databases
BioCyc	BHEN283166:BH05820-MON.
BRENDA	2.7.7.60. 277357. 4.6.1.12. 277357.
Family and domain databases
HAMAP	MF_01520. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. IPR003526. MECDP_synthase_core. IPR020555. MECDP_synthase_CS. [Graphical view]
Pfam	PF01128. IspD. 1 hit. PF02542. YgbB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. ispD. 1 hit. TIGR00151. ispF. 1 hit.
PROSITE	PS01295. ISPD. False negative. PS01350. ISPF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPDF_BARHE

Accession

Primary (citable) accession number: Q6G3Z8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	June 26, 2007
Last modified:	September 22, 2009
This is version 36 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)