Q6G3V1 (SYE2_BARHE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 2
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 2
Short name=GluRS 2

Gene names

Name:	gltX2
Ordered Locus Names:	BH06460

Organism

Bartonella henselae (strain ATCC 49882 / Houston 1) (Rochalimaea henselae) [Complete proteome] [HAMAP]

Taxonomic identifier

283166 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 459	459	Glutamate--tRNA ligase 2 HAMAP MF_00022_B		PRO_0000119511
Regions
	Motif	8 – 18	11	"HIGH" region HAMAP MF_00022_B
	Motif	249 – 253	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	252	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6G3V1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: CC9AB73BC04B3047	FASTA	459	52,847
	10 20 30 40 50 60 MIKVRFAPSP TGYIHIGNIR IALFNWLYAQ AHNGTFILRY DNTDVERSKQ EYIDAIAVDL 70 80 90 100 110 120 EWLGIQPDEI YYQSKRFNRY DEVAEILKQR GLLYPCYETA EELDRRRKIQ LSRKLPPVYD 130 140 150 160 170 180 RAALKLTPEK KREFETQGRK PHWRFLLPNF ENDPLQKKRT EVCWNDAVKG KQTIDLASLS 190 200 210 220 230 240 DPVLIREDGS YLYTLPSVVD DIDMAITHII RGDDHITNTG AQIALFEALN AKLPTFGHIN 250 260 270 280 290 300 LLTTLLGKGL SKRNNDLSIH SLRADGFESI AVQCLAVLIG TSQNVHPYPN QAVLLEHFNL 310 320 330 340 350 360 QDTSRSVAKF DIADLLTLNS HFVHELTYEE VKKRLENLSI NGEKVECFWN AIRSNINKVN 370 380 390 400 410 420 DAVLWWKMLH DEQNFDTVAL EDRAFVRQSL NLLPEGTLNE ESWKVWTVAL KEKTGRRGKA 430 440 450 LFMPLRQALT GMDHGPEMGK ILQLLGREKV IERLIIQGE

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49882 / Houston 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX897699 Genomic DNA. Translation: CAF27450.1.
RefSeq	YP_033475.1. NC_005956.1.
3D structure databases
ProteinModelPortal	Q6G3V1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2864754.
GenomeReviews	Gene locus BH06460 in contig BX897699_GR.
KEGG	bhe:BH06460.
NMPDR	fig|283166.1.peg.603.
PATRIC	20545109. VBIBarHen29080_0703.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	LLYPCYE.
ProtClustDB	PRK12558.
Enzyme and pathway databases
BioCyc	BHEN283166:BH06460-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE2_BARHE

Accession

Primary (citable) accession number: Q6G3V1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program