Q6G3K1 (SYT_BARHE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	BH07660

Organism

Bartonella henselae (strain ATCC 49882 / Houston 1) (Rochalimaea henselae) [Complete proteome] [HAMAP]

Taxonomic identifier

283166 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	658 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00184
Subunit structure	Homodimer By similarity. HAMAP MF_00184
Subcellular location	Cytoplasm HAMAP MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 658	658	Threonine--tRNA ligase HAMAP MF_00184		PRO_0000100941
Regions
	Region	246 – 549	304	Catalytic HAMAP MF_00184
Sites
	Metal binding	343	1	Zinc; catalytic By similarity
	Metal binding	394	1	Zinc; catalytic By similarity
	Metal binding	526	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6G3K1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: B6B8EAD5ABC325E2	FASTA	658	75,909
	10 20 30 40 50 60 MSCSVSLSFP DGSKRDYHSE MTGLELAESI SKSLAKKALA YSLNGIIRDL SDPIKQSGQI 70 80 90 100 110 120 EIITREDTRA LELIRHDCAH VLAEAVQELF PGTQVTIGPV IENGFYYDFA RQQPFTLDDL 130 140 150 160 170 180 TIIEKKMREI IQRNKPFRKE VWSRKKAREI FSEKGELYKV ELIDSIPDNQ DLKVYYQGDW 190 200 210 220 230 240 FDLCRGPHMQ STGQIGNAFK LMKVAGAYWR GDANNPMLTR IYGTAFANEN DLKAYLHMLE 250 260 270 280 290 300 EAEKRDHRRL GREMNLFHFQ EEGPGMIFWH KKGWKMFQNL INYMRRRLDD HQYAEVNAPQ 310 320 330 340 350 360 VLDRSLWEIS GHWEWYKENM FKAIPAAEDW DNENVYALKP MNCPGHVQIF KHGLKSYRDL 370 380 390 400 410 420 PTRIAEFGVV HRYEPSGSLH GLMRVRCFTQ DDAHIFCTDE QLADECLNIN DLILSTYADF 430 440 450 460 470 480 GFKEISLKLS TRPEKRVGSD ALWDHAENIM ESVLKTIETK SAGQIKTSIL PGEGAFYGPK 490 500 510 520 530 540 FEYTLKDAIG REWQCGTTQL DFNLPERFNA FYIDKDSEKR QPVMIHRAIF GSMERFLGIL 550 560 570 580 590 600 IENFSGHMPL WFAPEQIVVA TITSEANEYA KKLTAKLKSV GLSTTLDLRN EKINYKIREH 610 620 630 640 650 SLQKVPVILV CGKRESETNS VNMRRLGSTE QIFLSVEEVI EQLKNEATPP DLRRVINA

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49882 / Houston 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX897699 Genomic DNA. Translation: CAF27567.1.
RefSeq	YP_033578.1. NC_005956.1.
3D structure databases
ProteinModelPortal	Q6G3K1.
SMR	Q6G3K1. Positions 245-653.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2865222.
GenomeReviews	Gene locus BH07660 in contig BX897699_GR.
KEGG	bhe:BH07660.
NMPDR	fig|283166.1.peg.706.
PATRIC	20545348. VBIBarHen29080_0819.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG352811.
OMA	VGSDALW.
PhylomeDB	Q6G3K1.
ProtClustDB	PRK00413.
Enzyme and pathway databases
BioCyc	BHEN283166:BH07660-MONOMER.
Family and domain databases
HAMAP	MF_00184. Thr_tRNA_synth. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR012675. Beta-grasp_ferredoxin-type. IPR004095. TGS. IPR012676. TGS-like. IPR002320. Thr-tRNA-synth_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
KO	K01868.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF81271. TGS-like. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. ThrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_BARHE

Accession

Primary (citable) accession number: Q6G3K1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program