Reviewed, UniProtKB/Swiss-Prot Q6G2Z4 (SYA_BARHE)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	BH10220

Organism

Bartonella henselae (Rochalimaea henselae) [Complete proteome] [HAMAP]

Taxonomic identifier

38323 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	887 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm HAMAP MF_00036.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 887	887	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000075063

Sequences

	Sequence		Length	Mass (Da)
	Q6G2Z4-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 693ABFABFD861219	FASTA	887	97,711
	10 20 30 40 50 60 MNSVNSIRST FLDYFQLNGH KIVSSSPLVP RNDPTLMFTN AGMVQFKNVF TGLEQRPYKQ 70 80 90 100 110 120 ATTAQKCVRA GGKHNDLDNV GYTARHHTFF EMLGNFSFGD YFKEEAIFLS WNLLTKEFCL 130 140 150 160 170 180 PEDKLLVTVY HNDDVSTELW RKISGLPDEK IVRIATADNF WAMGDTGPCG PCSEIFYDHG 190 200 210 220 230 240 DEIWGGPPGS AYEDGDRFIE IWNLVFMQYE QVNKEKRVEL PHPSIDTGMG LERIAAVLQG 250 260 270 280 290 300 GHDNYDIDLF RALIAVSQEI TGVKATGDFI ASHRVIADHL RSSAFLIADG VLPSNEGRGY 310 320 330 340 350 360 VLRRIMRRAM RHAHLLGAKE PLMWRLLPVL IHEMGQAYPE LVRAESLISE TLKLEEIRFR 370 380 390 400 410 420 KTLERGLGLL SEASTDLKEG DHLNGEIAFK LYDTYGFPLD LTQDALRCRG ISVDVAAFNK 430 440 450 460 470 480 AMEGQKAKAR ANWSGSGETV TEAIWFSVRD QLGATEFLGY ETEKSEGILT ALVRDGEIVD 490 500 510 520 530 540 DISSGQKAIL VVNQTPFYGE SGGQIGDSGT ISGKNFVFEV HDTQKKADGV FIHIGEVKSG 550 560 570 580 590 600 QARMSECVEL TVDGVRRKKI RVNHSATHLL HEALRQVLGS HVTQKGSLVS PDRLRFDFSH 610 620 630 640 650 660 PKSVSLEELE KIEDLANEIV LQNSEVTTRL MLVDDAISEG AMALFGEKYG DEVRVVSMGN 670 680 690 700 710 720 RLEKGKLKSR WSIELCGGTH VERTGDIGLI HIVSESSVAA GVRRIEALTG TAARLYLSRQ 730 740 750 760 770 780 DARVHEIADL LKTSATDVEE RVRILLEDRR KLEKELNDER KKSVLSGGIV KSDQEDITII 790 800 810 820 830 840 NGISFMGRVV KNISPRDLKT LVDSGKKKIG SGVVAFIGVS EDGKGSAVLG VTDDLTHKLN 850 860 870 880 AVDLVRILSG ILGGQGGGGR PDMAQSGGPK GNKADEALAV LKASLEG

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49882 / Houston 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX897699 Genomic DNA. Translation: CAF27813.1.
RefSeq	YP_033806.1.
3D structure databases
SMR	Q6G2Z4. Positions 3-462, 489-708.
ModBase	Search...
Genome annotation databases
GeneID	2866067.
GenomeReviews	Gene locus BH10220 in contig BX897699_GR.
KEGG	bhe:BH10220.
NMPDR	fig|283166.1.peg.934.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG354397.
OMA	TLMFTNS.
PhylomeDB	Q6G2Z4.
Enzyme and pathway databases
BioCyc	BHEN283166:BH10220-MONOMER.
BRENDA	6.1.1.7. 277357.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_BARHE

Accession

Primary (citable) accession number: Q6G2Z4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	July 19, 2004
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)