Reviewed, UniProtKB/Swiss-Prot Q6G2S8 (TYSY_BARHE)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase
      Short name=TS
      Short name=TSase
    EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	BH10980

Organism

Bartonella henselae (Rochalimaea henselae) [Complete proteome] [HAMAP]

Taxonomic identifier

38323 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type thyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Thymidylate synthase HAMAP MF_00008		PRO_0000140934
Sites
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6G2S8-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: F8AD5E04F57D5C14	FASTA	264	30,294
	10 20 30 40 50 60 MKTYLDLLSH VLNKGIDRTD RTGVGTRSVF GYQMRFDLQA GFPLLTTKKL HLRSIIYELL 70 80 90 100 110 120 WFLRGDTNIA WLKEHGVSIW DEWADEKGNL GPIYGYQWRS WPAPDGRHID QISNLLMMIK 130 140 150 160 170 180 KTPDSRRLIV SAWNPASIKE MALPPCHCFF QFYVADGKLS CQLYQRSADI FLGIPFNIAS 190 200 210 220 230 240 YALLTMMIAQ VSGLKVGDFI HTLGDAHLYS NHFEQAQHQL SRIPNALPFM RINPAVTDLF 250 260 SFKFEDFELL NYDAQPHIKA PIAV

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49882 / Houston 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX897699 Genomic DNA. Translation: CAF27884.1.
RefSeq	YP_033873.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2865223.
GenomeReviews	Gene locus BH10980 in contig BX897699_GR.
KEGG	bhe:BH10980.
NMPDR	fig|283166.1.peg.1001.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6G2S8.
OMA	LGPVYGH.
Enzyme and pathway databases
BioCyc	BHEN283166:BH10980-MON.
BRENDA	2.1.1.45. 277357.
Family and domain databases
HAMAP	MF_00008. [Tree]
InterPro	IPR000398. Thymidylat_synth_C. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
ProDom	PD001180. Thymidylat_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_BARHE

Accession

Primary (citable) accession number: Q6G2S8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	July 19, 2004
Last modified:	November 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)