Reviewed, UniProtKB/Swiss-Prot Q6G2R5 (DNLJ_BARHE)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.2
Alternative name(s):
    Polydeoxyribonucleotide synthase [NAD+]

Gene names

Name:	ligA
Ordered Locus Names:	BH11140

Organism

Bartonella henselae (Rochalimaea henselae) [Complete proteome] [HAMAP]

Taxonomic identifier

38323 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	716 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA By similarity.
Catalytic activity	NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m). HAMAP MF_01588
Cofactor	Magnesium or manganese By similarity.
Sequence similarities	Belongs to the NAD-dependent DNA ligase family. LigA subfamily. Contains 1 BRCT domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Ligand	Magnesium Manganese Metal-binding NAD Zinc
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (NAD+) activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 716	716	DNA ligase HAMAP MF_01588		PRO_0000313135
Regions
	Domain	638 – 716	79	BRCT
	Nucleotide binding	42 – 46	5	NAD By similarity
	Nucleotide binding	91 – 92	2	NAD By similarity
Sites
	Active site	127	1	N6-AMP-lysine intermediate By similarity
	Metal binding	429	1	Zinc By similarity
	Metal binding	432	1	Zinc By similarity
	Metal binding	447	1	Zinc By similarity
	Metal binding	453	1	Zinc By similarity
	Binding site	125	1	NAD By similarity
	Binding site	148	1	NAD By similarity
	Binding site	184	1	NAD By similarity
	Binding site	300	1	NAD By similarity
	Binding site	324	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6G2R5-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: DE6D1FA3074BAAEA	FASTA	716	79,736
	10 20 30 40 50 60 MHKDEIQNLT ALEAASELEW LAKEITRHDV LYNRNDQPEI SDAEYDALRR RNAEIEALFP 70 80 90 100 110 120 ELIRPDSPSH KIGAPISEKF EKSVHSQPML SLDNAFSSED VSEFMERVRR FLRLPETQML 130 140 150 160 170 180 EITAEPKIDG LSLSLRYEQG RLVCAATRGD GYVGENVTAN ARTISDIPQV LQGKFPDIIE 190 200 210 220 230 240 VRGEVYMRRA DFQALNVNQQ EKGKLAFANP RNAAAGSLRQ LDSRITASRK LQFFAYACGE 250 260 270 280 290 300 VSEIFAESQM GMMEKLKEYG FVINPLIKKI KSLEELISYY HDIEERRHAL SYDIDGIVYK 310 320 330 340 350 360 VNDLMLQMRL GFVSRSPRWA IAHKFPAEKA MTVLEGIDIQ VGRTGALTPV ARLAPITVGG 370 380 390 400 410 420 VVITNASLHN EDYIKGIGSK GEPIREGNDI RVGDTVIVQR AGDVIPQIID IVAEKRSKDA 430 440 450 460 470 480 SAFVFPYLCP ACGSYAVREI GEAVRRCTGG LICPAQAIER IRHFVSRNAF DIEGLGKKQV 490 500 510 520 530 540 EFFFQIQDET LCIHTPADIF TLQRRQEKSL VHLENMEGFG TVSVRKLYDA INARRKIPLS 550 560 570 580 590 600 RFLFALGIRY VGEVNARRLA RAYQNYTAFE TAAMAATMPD DKVGKEGNEA WMELTNIEGI 610 620 630 640 650 660 GPQVGEAIID FYKEVHNREV LSGLLCEVTP LDEESVMTAS SPIAGKIIVF TGTLTRMSRD 670 680 690 700 710 EAKALAERLG AKTSGSLSKK TNLLVAGVGG GSKLTKAQEL GVEVIDEEAW LQLIEG

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49882 / Houston 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX897699 Genomic DNA. Translation: CAF27899.1.
RefSeq	YP_033886.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2865583.
GenomeReviews	Gene locus BH11140 in contig BX897699_GR.
KEGG	bhe:BH11140.
NMPDR	fig|283166.1.peg.1014.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6G2R5.
OMA	YKFPAQE.
Enzyme and pathway databases
BioCyc	BHEN283166:BH11140-MON.
BRENDA	6.5.1.2. 277357.
Family and domain databases
HAMAP	MF_01588. [Tree]
InterPro	IPR001357. BRCT. IPR018239. DNA_ligase_AS. IPR004150. DNA_ligase_OB. IPR001679. DNAligase. IPR013839. DNAligase_adenylation. IPR013840. DNAligase_N. IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif. IPR012340. NA-bd_OB-fold. IPR004149. Znf_DNAligase_C4. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF00533. BRCT. 1 hit. PF01653. DNA_ligase_aden. 1 hit. PF03120. DNA_ligase_OB. 1 hit. PF03119. DNA_ligase_ZBD. 1 hit. [Graphical view]
PIRSF	PIRSF001604. LigA. 1 hit.
ProDom	PD003944. DNAligase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00292. BRCT. 1 hit. SM00278. HhH1. 2 hits. SM00532. LIGANc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00575. dnlj. 1 hit.
PROSITE	PS50172. BRCT. 1 hit. PS01055. DNA_LIGASE_N1. 1 hit. PS01056. DNA_LIGASE_N2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLJ_BARHE

Accession

Primary (citable) accession number: Q6G2R5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 19, 2004
Last modified:	November 3, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)