Q6G202 (DUT_BARHE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Name:	dut
Ordered Locus Names:	BH14690

Organism

Bartonella henselae (strain ATCC 49882 / Houston 1) (Rochalimaea henselae) [Complete proteome] [HAMAP]

Taxonomic identifier

283166 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella › Bartonella henselae

Protein attributes

Sequence length	184 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP-Rule MF_00116
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP-Rule MF_00116
Cofactor	Magnesium By similarity.
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP-Rule MF_00116
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	dUMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	dUTP diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 184	184	Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP-Rule MF_00116		PRO_0000182827
Regions
	Region	96 – 98	3	Substrate binding By similarity
	Region	113 – 115	3	Substrate binding By similarity
Sites
	Binding site	109	1	Substrate By similarity
	Binding site	123	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6G202 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 52E685DB3ADEAC37	FASTA	184	19,660
	10 20 30 40 50 60 MPHTQTDAHQ NNQENFSSSL ISSRPSQPMT LSIQRLDHGQ GLELPHYATA GSAGLDLRAA 70 80 90 100 110 120 LSEEETVILA PGQRALIPTG LIFHLSPGFE AQIRPRSGLA LKHGITCLNT PGTIDSDYRG 130 140 150 160 170 180 EVKVLLINCG QEDFAIKRGM RIAQTVIAPV LQVNVCALEP QQNESTKNTV GNRGAGGFGS TGHD

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49882 / Houston 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX897699 Genomic DNA. Translation: CAF28232.1.
RefSeq	YP_034169.1. NC_005956.1.
3D structure databases
ProteinModelPortal	Q6G202.
ModBase	Search...
Protein-protein interaction databases
STRING	283166.BH14690.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAF28232; CAF28232; BH14690.
GeneID	2865143.
KEGG	bhe:BH14690.
PATRIC	20547132. VBIBarHen29080_1674.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0756.
HOGENOM	HOG000028968.
KO	K01520.
OMA	NICSDAR.
ProtClustDB	PRK00601.
Enzyme and pathway databases
BioCyc	BHEN283166:GIVZ-1464-MONOMER.
UniPathway	UPA00610; UER00666.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact.
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_BARHE

Accession

Primary (citable) accession number: Q6G202

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	July 19, 2004
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program