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Protein

tRNA (guanine-N(1)-)-methyltransferase

Gene

trmD

Organism
Bartonella henselae (strain ATCC 49882 / DSM 28221 / Houston 1) (Rochalimaea henselae)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates guanosine-37 in various tRNAs.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei111S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(1)-)-methyltransferaseUniRule annotation (EC:2.1.1.228UniRule annotation)
Alternative name(s):
M1G-methyltransferaseUniRule annotation
tRNA [GM37] methyltransferaseUniRule annotation
Gene namesi
Name:trmDUniRule annotation
Ordered Locus Names:BH15820
OrganismiBartonella henselae (strain ATCC 49882 / DSM 28221 / Houston 1) (Rochalimaea henselae)
Taxonomic identifieri283166 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBartonellaceaeBartonella
Proteomesi
  • UP000000421 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000603311 – 232tRNA (guanine-N(1)-)-methyltransferaseAdd BLAST232

Proteomic databases

PaxDbiQ6G1R9.
PRIDEiQ6G1R9.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi283166.BH15820.

Structurei

Secondary structure

1232
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 10Combined sources8
Helixi12 – 14Combined sources3
Helixi17 – 20Combined sources4
Helixi22 – 29Combined sources8
Beta strandi32 – 39Combined sources8
Helixi40 – 43Combined sources4
Helixi66 – 73Combined sources8
Beta strandi82 – 85Combined sources4
Beta strandi89 – 91Combined sources3
Helixi94 – 100Combined sources7
Beta strandi103 – 110Combined sources8
Helixi118 – 123Combined sources6
Beta strandi127 – 134Combined sources8
Helixi139 – 151Combined sources13
Beta strandi157 – 159Combined sources3
Helixi160 – 162Combined sources3
Helixi168 – 171Combined sources4
Beta strandi181 – 185Combined sources5
Helixi192 – 195Combined sources4
Helixi199 – 217Combined sources19
Helixi219 – 228Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3IEFX-ray2.50A/B1-232[»]
ProteinModelPortaliQ6G1R9.
SMRiQ6G1R9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6G1R9.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni131 – 136S-adenosyl-L-methionine bindingUniRule annotation6

Sequence similaritiesi

Belongs to the RNA methyltransferase TrmD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D6X. Bacteria.
COG0336. LUCA.
HOGENOMiHOG000016242.
KOiK00554.
OMAiYKGVDQR.
OrthoDBiPOG091H01WE.

Family and domain databases

Gene3Di1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPiMF_00605. TrmD. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamiPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFiPIRSF000386. tRNA_mtase. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00088. trmD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6G1R9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFQARVLTL YPEMFPGFLG CSLAGQALKQ GIWSLETVQI RDFALDKHHS
60 70 80 90 100
VDDTPAGGGA GMVMRADVLA AALDSCPNDS PRLLMSPRGR LLNQAYARSL
110 120 130 140 150
ARSSGVTLVC GRFEGVDERI IEARELEEVS IGDYILSGGE TAALVLLDAI
160 170 180 190 200
VRLLPGVMGN EISAKCESFE NGLLEHPQYT RPAVFEGRGI PPVLTSGHHK
210 220 230
AIANWRQQQA ESLTRQRRPD LYALYNKNRQ KT
Length:232
Mass (Da):25,463
Last modified:July 19, 2004 - v1
Checksum:i4C89D122DB57C9BB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX897699 Genomic DNA. Translation: CAF28345.1.
RefSeqiWP_011181348.1. NZ_LRIJ01000002.1.

Genome annotation databases

EnsemblBacteriaiCAF28345; CAF28345; BH15820.
KEGGibhe:BH15820.
PATRICi20547460. VBIBarHen29080_1838.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX897699 Genomic DNA. Translation: CAF28345.1.
RefSeqiWP_011181348.1. NZ_LRIJ01000002.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3IEFX-ray2.50A/B1-232[»]
ProteinModelPortaliQ6G1R9.
SMRiQ6G1R9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi283166.BH15820.

Proteomic databases

PaxDbiQ6G1R9.
PRIDEiQ6G1R9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAF28345; CAF28345; BH15820.
KEGGibhe:BH15820.
PATRICi20547460. VBIBarHen29080_1838.

Phylogenomic databases

eggNOGiENOG4105D6X. Bacteria.
COG0336. LUCA.
HOGENOMiHOG000016242.
KOiK00554.
OMAiYKGVDQR.
OrthoDBiPOG091H01WE.

Miscellaneous databases

EvolutionaryTraceiQ6G1R9.

Family and domain databases

Gene3Di1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPiMF_00605. TrmD. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamiPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFiPIRSF000386. tRNA_mtase. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00088. trmD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRMD_BARHE
AccessioniPrimary (citable) accession number: Q6G1R9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: July 19, 2004
Last modified: November 2, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.