Reviewed, UniProtKB/Swiss-Prot Q6G1R9 (TRMD_BARHE)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase
    EC=2.1.1.31
Alternative name(s):
    M1G-methyltransferase
    tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	BH15820

Organism

Bartonella henselae (Rochalimaea henselae) [Complete proteome] [HAMAP]

Taxonomic identifier

38323 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. HAMAP MF_00605
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA methyltransferase trmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 232	232	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000060331
Regions
	Region	131 – 136	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	111	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6G1R9-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 4C89D122DB57C9BB	FASTA	232	25,463
	10 20 30 40 50 60 MKFQARVLTL YPEMFPGFLG CSLAGQALKQ GIWSLETVQI RDFALDKHHS VDDTPAGGGA 70 80 90 100 110 120 GMVMRADVLA AALDSCPNDS PRLLMSPRGR LLNQAYARSL ARSSGVTLVC GRFEGVDERI 130 140 150 160 170 180 IEARELEEVS IGDYILSGGE TAALVLLDAI VRLLPGVMGN EISAKCESFE NGLLEHPQYT 190 200 210 220 230 RPAVFEGRGI PPVLTSGHHK AIANWRQQQA ESLTRQRRPD LYALYNKNRQ KT

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49882 / Houston 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX897699 Genomic DNA. Translation: CAF28345.1.
RefSeq	YP_034278.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2865240.
GenomeReviews	Gene locus BH15820 in contig BX897699_GR.
KEGG	bhe:BH15820.
NMPDR	fig|283166.1.peg.1406.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6G1R9.
OMA	Q6G1R9. HRSVDDT.
Enzyme and pathway databases
BioCyc	BHEN283166:BH15820-MON.
BRENDA	2.1.1.31. 277357.
Family and domain databases
HAMAP	MF_00605. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. [Graphical view]
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
ProDom	PD004978. tRNA_m1G_mtfrase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00088. trmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_BARHE

Accession

Primary (citable) accession number: Q6G1R9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	July 19, 2004
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)