Q6G1M0 (MDH_BARHE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	BH16570

Organism

Bartonella henselae (strain ATCC 49882 / Houston 1) (Rochalimaea henselae) [Complete proteome] [HAMAP]

Taxonomic identifier

283166 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP MF_00487
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Malate dehydrogenase HAMAP MF_00487		PRO_0000113437
Regions
	Nucleotide binding	10 – 15	6	NAD By similarity
	Nucleotide binding	119 – 121	3	NAD By similarity
Sites
	Active site	176	1	Proton acceptor By similarity
	Binding site	34	1	NAD By similarity
	Binding site	83	1	Substrate By similarity
	Binding site	89	1	Substrate By similarity
	Binding site	96	1	NAD By similarity
	Binding site	121	1	Substrate By similarity
	Binding site	152	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6G1M0 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 24C2AFA4B2A2C720	FASTA	320	33,664
	10 20 30 40 50 60 MARKKIALIG SGMIGGTLAH IIGLKELGDV VLFDIAEGIP QGKALDIAES SPVDGFDVSL 70 80 90 100 110 120 TGANSYDVIE GADVVIVTAG VARKPGMSRD DLLGINLKVM EQVGAGIKKY ASSAFVICIT 130 140 150 160 170 180 NPLDAMVWAL QKFSGLPTQK VVGMAGILDS ARFRHFLSEE FKISVKDVTA FVLGGHGDSM 190 200 210 220 230 240 VPLVRYSTVG GISLPDLVKM GWTTQEKIDQ IIQRTRDGGA EIVSLLKTGS AFYAPAASAV 250 260 270 280 290 300 SMAEAYLKDT KRVVPVAAYL SGQYGVKDTY VGVPVVIGAG GVERVIEIDL DKEEKAAFEK 310 320 SVSAVQKLCE ACIAVAPGLK

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49882 / Houston 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX897699 Genomic DNA. Translation: CAF28418.1.
RefSeq	YP_034347.1. NC_005956.1.
3D structure databases
ProteinModelPortal	Q6G1M0.
SMR	Q6G1M0. Positions 3-311.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2865615.
GenomeReviews	Gene locus BH16570 in contig BX897699_GR.
KEGG	bhe:BH16570.
NMPDR	fig|283166.1.peg.1475.
PATRIC	20547640. VBIBarHen29080_1927.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG566126.
OMA	ANSYEAI.
PhylomeDB	Q6G1M0.
ProtClustDB	PRK06223.
Enzyme and pathway databases
BioCyc	BHEN283166:BH16570-MONOMER.
Family and domain databases
HAMAP	MF_00487. Malate_dehydrog_3. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_type3. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00024.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01763. MalateDH_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_BARHE

Accession

Primary (citable) accession number: Q6G1M0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program