Q6G1L3 (GLO2_BARHE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6

Alternative name(s):
Glyoxalase II
Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	BH16640

Organism

Bartonella henselae (Rochalimaea henselae) [Complete proteome] [HAMAP]

Taxonomic identifier

38323 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_1000068208
Sites
	Metal binding	54	1	Zinc 1 By similarity
	Metal binding	56	1	Zinc 1 By similarity
	Metal binding	58	1	Zinc 2 By similarity
	Metal binding	59	1	Zinc 2 By similarity
	Metal binding	112	1	Zinc 1 By similarity
	Metal binding	131	1	Zinc 1 By similarity
	Metal binding	131	1	Zinc 2 By similarity
	Metal binding	169	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6G1L3-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 9EA78604E4B571D1	FASTA	253	29,075
	10 20 30 40 50 60 MLIEQFICRE DNFGVLIHDE TSGYTAAIDA PESNAIQNAL KRRNWTLQTI FLTHHHHDHV 70 80 90 100 110 120 EALAELKQIY KAIVIGPEAE KEKISHIDQT LKPDESFLFG THTILALSTP GHTLGALSYY 130 140 150 160 170 180 FPQENLLFAG DTLFSLGCGR LFEGTPAQML NSLKKLRQLP DETLLYCGHE YTKSNALFAL 190 200 210 220 230 240 TLDPYNQKLQ QRAEDVFLLR AKNAMTLPVT LGQEKKNNPF LRWDNRTLRK TLRMEKETDE 250 EVFAEIRKRK DNF

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49882 / Houston 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX897699 Genomic DNA. Translation: CAF28425.1.
RefSeq	YP_034354.1.
3D structure databases
HSSP	HSSP built from PDB template 1XM8 based on UniProtKB Q9SID3.
ProteinModelPortal	Q6G1L3.
SMR	Q6G1L3. Positions 1-253.
ModBase	Search...
Genome annotation databases
GeneID	2864746.
GenomeReviews	Gene locus BH16640 in contig BX897699_GR.
KEGG	bhe:BH16640.
NMPDR	fig|283166.1.peg.1482.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG753931.
OMA	WCAHEYT.
PhylomeDB	Q6G1L3.
ProtClustDB	CLSK927387.
Enzyme and pathway databases
BioCyc	BHEN283166:BH16640-MONOMER.
BRENDA	3.1.2.6. 277357.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Blactmase-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_BARHE

Accession

Primary (citable) accession number: Q6G1L3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	July 19, 2004
Last modified:	August 10, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)