Reviewed, UniProtKB/Swiss-Prot Q6G164 (ISPDF_BARQU)

Names and origin

Protein names

Recommended name:
    Bifunctional enzyme ispD/ispF
Including the following 2 domains:
    1- Recommended name:
            2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
              EC=2.7.7.60
        Alternative name(s):
            4-diphosphocytidyl-2C-methyl-D-erythritol synthase
            MEP cytidylyltransferase
              Short name=MCT
    2- Recommended name:
            2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
                Short name=MECPS
                Short name=MECDP-synthase
              EC=4.6.1.12

Gene names

Name:	ispDF
Ordered Locus Names:	BQ04980

Organism

Bartonella quintana (Rochalimaea quintana) [Complete proteome] [HAMAP]

Taxonomic identifier

803 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (ispD), and converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP (ispF) By similarity.
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_01520 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP. HAMAP MF_01520
Cofactor	Divalent metal cations By similarity.
Pathway	Isoprenoid biosynthesis; isopentenyl-PP biosynthesis via DXP pathway; isopentenyl-PP from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_01520 Isoprenoid biosynthesis; isopentenyl-PP biosynthesis via DXP pathway; isopentenyl-PP from 1-deoxy-D-xylulose 5-phosphate: step 4/6.
Sequence similarities	In the N-terminal section; belongs to the ispD family. In the C-terminal section; belongs to the ispF family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding
Molecular function	Lyase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity Inferred from electronic annotation. Source: HAMAP 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 391	391	Bifunctional enzyme ispD/ispF HAMAP MF_01520		PRO_0000075657
Regions
	Region	1 – 230	230	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_01520
	Region	231 – 391	161	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase HAMAP MF_01520
Sites
	Metal binding	237	1	Divalent metal cation By similarity
	Metal binding	239	1	Divalent metal cation By similarity
	Metal binding	271	1	Divalent metal cation By similarity
	Site	9	1	Transition state stabilizer By similarity
	Site	18	1	Transition state stabilizer By similarity
	Site	149	1	Positions MEP for the nucleophilic attack By similarity
	Site	206	1	Positions MEP for the nucleophilic attack By similarity
	Site	263	1	Transition state stabilizer By similarity
	Site	362	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6G164-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: E81E1DC69C3B2B75	FASTA	391	43,792
	10 20 30 40 50 60 MLAAGRGKRA GSLQKNPKQY RLLGQKPVIC HTVRCFCQHP AITTIILVIH PEDRQICEQA 70 80 90 100 110 120 ITDFKEHLII VEGGNTRQIS TLRGLHALKK FKPKYVHIHD GARPFIENKL LEKIHTTVNH 130 140 150 160 170 180 QEGVLPVLPV SDTLKRVNST HRVLETIPHT HLYSAQTPQC FPFERILAAH ERAMQTCKKE 190 200 210 220 230 240 FTDDSAIAEW FGIPMHTIPG DSHNIKITWH EDFDTAHLYL KKKMQMFPDI RTGNGYDVHS 250 260 270 280 290 300 FEEGTSLILC GIKIPFHKKL KGHSDADVAF HALTDALLAT QGAGDIGTHF LPSDPQWKNA 310 320 330 340 350 360 PSEIFLRHAL EIIKQAGGRI ANVDITLIAE TPKIGPYRHT MTENLMNILS LSLDRISIKA 370 380 390 TTNEKLGFIG REEGIAALAT ATVLYPGEIP K

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Toulouse.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX897700 Genomic DNA. Translation: CAF25997.1.
RefSeq	YP_032176.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2866390.
GenomeReviews	Gene locus BQ04980 in contig BX897700_GR.
KEGG	bqu:BQ04980.
NMPDR	fig|283165.1.peg.446.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6G164.
OMA	Q6G164. IVLIHDA.
Enzyme and pathway databases
BioCyc	BQUI283165:BQ04980-MON.
BRENDA	2.7.7.60. 292235. 4.6.1.12. 292235.
Family and domain databases
HAMAP	MF_01520. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. IPR003526. MECDP_synthase_core. [Graphical view]
Gene3D	G3DSA:3.30.1330.50. MECDP_synthase_core. 1 hit.
Pfam	PF01128. IspD. 1 hit. PF02542. YgbB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. ispD. 1 hit. TIGR00151. ispF. 1 hit.
PROSITE	PS01295. ISPD. False negative. PS01350. ISPF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPDF_BARQU

Accession

Primary (citable) accession number: Q6G164

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	July 19, 2004
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)