Reviewed, UniProtKB/Swiss-Prot Q6G159 (PYRD_BARQU)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.5.2
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	BQ02890

Organism

Bartonella quintana (Rochalimaea quintana) [Complete proteome] [HAMAP]

Taxonomic identifier

803 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + a quinone = orotate + a quinol. HAMAP MF_00225
Cofactor	Binds 1 FMN per subunit By similarity. HAMAP MF_00225
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1. HAMAP MF_00225
Subunit structure	Homodimer By similarity. HAMAP MF_00225
Subcellular location	Cell membrane; Peripheral membrane protein By similarity HAMAP MF_00225.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	Dihydroorotate dehydrogenase HAMAP MF_00225		PRO_0000336455
Sites
	Active site	171	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6G159-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 31EEA7729A643ED0	FASTA	362	40,178
	10 20 30 40 50 60 MSFFRCIGRF ALFMLDPEHA HRLAIVGLKS GLNSYRKVVD RRLCLTVAGL EFENFVGLAA 70 80 90 100 110 120 GFDKNAEVVE AIFHLGFGFT EIGTVTPKPQ VGNPKPRLFR LREDEAIINR MGFNNNGHHI 130 140 150 160 170 180 VYSRLCACKK LGIVGINIGA NKDTVDKIDD YATGIACFYD IADYFTVNIS SPNTPGLRDL 190 200 210 220 230 240 QARDSLHLLM NALSQARREQ KKKHGFSVPI FLKIAPDLSE KELDDVAEEM KLSDFDGLIV 250 260 270 280 290 300 SNTTLSRQGL RDSKLSGEKG GLSGCPLFER STIVLAKMRQ KLGKEIAIIG VGGIKDAQTA 310 320 330 340 350 360 LEKIKAGADL IQLYSGIVYE GPDLAITILK EILQFMQQDG VDTIKTYRDH RVEYWAKRAL PL

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Toulouse.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX897700 Genomic DNA. Translation: CAF25790.1.
RefSeq	YP_031980.1.
3D structure databases
HSSP	HSSP built from PDB template 1D3G based on UniProtKB Q02127.
SMR	Q6G159. Positions 4-332.
ModBase	Search...
Genome annotation databases
GeneID	2867078.
GenomeReviews	Gene locus BQ02890 in contig BX897700_GR.
KEGG	bqu:BQ02890.
NMPDR	fig|283165.1.peg.250.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351027.
OMA	AALNRMG.
PhylomeDB	Q6G159.
Enzyme and pathway databases
BioCyc	BQUI283165:BQ02890-MONOMER.
Family and domain databases
HAMAP	MF_00225. DHO_dh_type2. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005719. Dihydroorotate_DH_2. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_BARQU

Accession

Primary (citable) accession number: Q6G159

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	July 19, 2004
Last modified:	January 19, 2010
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)