Reviewed, UniProtKB/Swiss-Prot Q6G0Y4 (SYFA_BARQU)

Names and origin

Protein names

Recommended name:
    Phenylalanyl-tRNA synthetase alpha chain
    EC=6.1.1.20
Alternative name(s):
    Phenylalanine--tRNA ligase alpha chain
      Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	BQ00770

Organism

Bartonella quintana (Rochalimaea quintana) [Complete proteome] [HAMAP]

Taxonomic identifier

803 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity. HAMAP MF_00281
Subcellular location	Cytoplasm HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00281		PRO_0000231965
Sites
	Metal binding	260	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6G0Y4-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 713D6B314F10C9B6	FASTA	361	41,511
	10 20 30 40 50 60 MNDIERLEKE ICLALEAASD EQTLETVRIA ALGKKGCISE KLKALGKMDV EERHKVGPVL 70 80 90 100 110 120 NGLKNRVLEL WVQKRDLLRR QAMNKRLSRE TVDVTLPVRS SPLERGRIHP ISQVIEEIIA 130 140 150 160 170 180 IYANMGFSLA EGPDIETDYY NFTALNFPEG HPAREMHDTF FFDVDKTGER KLLRTHTSPV 190 200 210 220 230 240 QIRTMEKQKA PIRIIIPGKT YRMDSDATHS PMFHQVEGLV IDKTSTIAHM MWLHETFCKA 250 260 270 280 290 300 FFEVPSVKMR FRPSFFPFTE PSMEVDIQCD RSGSKVKFGE GQDWLEILGC GMVHPYVLQN 310 320 330 340 350 360 VGLDPDVYQG FAWGMGIDRI AMLKYGMPDL RAFFDADLRW LDHYGFRCFD MPAFFPGLRN E

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Toulouse.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX897700 Genomic DNA. Translation: CAF25584.1.
RefSeq	YP_031802.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2867409.
GenomeReviews	Gene locus BQ00770 in contig BX897700_GR.
KEGG	bqu:BQ00770.
NMPDR	fig|283165.1.peg.72.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG284353.
OMA	FRASYFP.
PhylomeDB	Q6G0Y4.
Enzyme and pathway databases
BioCyc	BQUI283165:BQ00770-MONOMER.
BRENDA	6.1.1.20. 292235.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-dom. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR002319. Phenylalanyl-tRNA_Synthase_acu. IPR010978. tRNA_bd_arm. [Graphical view]
PANTHER	PTHR11538. tRNA-synt_2d. 1 hit.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_BARQU

Accession

Primary (citable) accession number: Q6G0Y4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	July 19, 2004
Last modified:	February 9, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)