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Q6G0K6

- SYI_BARQU

UniProt

Q6G0K6 - SYI_BARQU

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Protein

Isoleucine--tRNA ligase

Gene
ileS, BQ02440
Organism
Bartonella quintana (strain Toulouse) (Rochalimaea quintana)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei602 – 6021Aminoacyl-adenylate By similarity
Binding sitei646 – 6461ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBQUI283165:GHZA-244-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:BQ02440
OrganismiBartonella quintana (strain Toulouse) (Rochalimaea quintana)
Taxonomic identifieri283165 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBartonellaceaeBartonella
ProteomesiUP000000597: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 971971Isoleucine--tRNA ligaseUniRule annotationPRO_0000098354Add
BLAST

Proteomic databases

PRIDEiQ6G0K6.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi283165.BQ02440.

Structurei

3D structure databases

ProteinModelPortaliQ6G0K6.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi64 – 7411"HIGH" regionUniRule annotationAdd
BLAST
Motifi643 – 6475"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6G0K6-1 [UniParc]FASTAAdd to Basket

« Hide

MTVKNETIDY SKTLYLPQTN FPMRGRLPQK ELELIARWDN MGLYAQLRQQ    50
AKDRPLYILH DGPPYANGHI HIGHALNKVL KDVIIRSFQM RGFNANYVPG 100
WDCHGLPIEW KIEEKYRAQG KNKDDVPLNE FRQECREFAK YWITVQSEEF 150
KRLGVVGDFN SPYTTMAFHA EARIASELMK FAMSGQIYRG SKPVMWSVVE 200
RTALAEAEIE YHDYESEVIW VKFPVLQADS SDLCGAYVVI WTTTPWTIPG 250
NRAVSYSSQI SYGVYEVAST QNDFGPQVGE RLLFADALAM SCAEKAKLVL 300
KRLRIISADE LKTLFLFHPL KGFAGSYNDK IAMLDGAHVT ENAGTGFVHT 350
APSHGREDFE IWNAYKPLLE QFGIDSSIPF PVDDAGFYTK DAPGFGPNRE 400
GGAVRVIDDN GKMGDANKEV INALIKADRL FARGRLKHSY PHSWRSKKPV 450
IFRNTPQWFI SMDKDLGDGS TLRSRALEAV SMTRFVPSSG QSRLASMIAD 500
RPDWVLSRQR AWGVPICIFA NEDGVILKDE RVNERILRAF EAEGADAWFA 550
EGARERFLGE RAHESWVQVL DILDVWFDSG ASHSFVLEDR DDLKWPADVY 600
FEGSDQHRGW FQSSLLESCG TRACSPYKAV ITHGFTLDEN GKKMSKSLGN 650
TVVPQEIIKT SGADIFRLWV MTTDYWEDQR LGKQILQTNM DLYRKLRNAI 700
RWMLGILAHD EGEKISYCAL PDLEKFILHQ LFELDQLINR AYDEFDFKKI 750
MRALLDFSIT ELSAFYFDIR KDSLYCDAPS SKKRKASLQV IREIFERMVI 800
WLAPMLPFTM EEAWLEHSPK SHSVHLEQFR SVPGEWQNGF LAERWRKVRQ 850
VRKVVTGALE FERAAKRIGS SLEAAPIVFI SNPVLLEALE NLDMAEICIT 900
SALTITQDVP PSDAFILSDV EGVGVYPGKA LGTKCARSWR YTQDVGSDPA 950
YPDVSARDAA ALRELQMLGK I 971
Length:971
Mass (Da):110,443
Last modified:July 19, 2004 - v1
Checksum:iC7478D6326045340
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX897700 Genomic DNA. Translation: CAF25747.1.
RefSeqiYP_031948.1. NC_005955.1.

Genome annotation databases

EnsemblBacteriaiCAF25747; CAF25747; BQ02440.
GeneIDi2866644.
KEGGibqu:BQ02440.
PATRICi31951693. VBIBarQui58630_0286.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX897700 Genomic DNA. Translation: CAF25747.1 .
RefSeqi YP_031948.1. NC_005955.1.

3D structure databases

ProteinModelPortali Q6G0K6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 283165.BQ02440.

Proteomic databases

PRIDEi Q6G0K6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAF25747 ; CAF25747 ; BQ02440 .
GeneIDi 2866644.
KEGGi bqu:BQ02440.
PATRICi 31951693. VBIBarQui58630_0286.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BQUI283165:GHZA-244-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Toulouse.

Entry informationi

Entry nameiSYI_BARQU
AccessioniPrimary (citable) accession number: Q6G0K6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 19, 2004
Last modified: May 14, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi