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Q6G0K6

- SYI_BARQU

UniProt

Q6G0K6 - SYI_BARQU

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Bartonella quintana (strain Toulouse) (Rochalimaea quintana)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (19 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei602 – 6021Aminoacyl-adenylateUniRule annotation
    Binding sitei646 – 6461ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBQUI283165:GHZA-244-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:BQ02440
    OrganismiBartonella quintana (strain Toulouse) (Rochalimaea quintana)
    Taxonomic identifieri283165 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBartonellaceaeBartonella
    ProteomesiUP000000597: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 971971Isoleucine--tRNA ligasePRO_0000098354Add
    BLAST

    Proteomic databases

    PRIDEiQ6G0K6.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi283165.BQ02440.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6G0K6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi64 – 7411"HIGH" regionAdd
    BLAST
    Motifi643 – 6475"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6G0K6-1 [UniParc]FASTAAdd to Basket

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    MTVKNETIDY SKTLYLPQTN FPMRGRLPQK ELELIARWDN MGLYAQLRQQ    50
    AKDRPLYILH DGPPYANGHI HIGHALNKVL KDVIIRSFQM RGFNANYVPG 100
    WDCHGLPIEW KIEEKYRAQG KNKDDVPLNE FRQECREFAK YWITVQSEEF 150
    KRLGVVGDFN SPYTTMAFHA EARIASELMK FAMSGQIYRG SKPVMWSVVE 200
    RTALAEAEIE YHDYESEVIW VKFPVLQADS SDLCGAYVVI WTTTPWTIPG 250
    NRAVSYSSQI SYGVYEVAST QNDFGPQVGE RLLFADALAM SCAEKAKLVL 300
    KRLRIISADE LKTLFLFHPL KGFAGSYNDK IAMLDGAHVT ENAGTGFVHT 350
    APSHGREDFE IWNAYKPLLE QFGIDSSIPF PVDDAGFYTK DAPGFGPNRE 400
    GGAVRVIDDN GKMGDANKEV INALIKADRL FARGRLKHSY PHSWRSKKPV 450
    IFRNTPQWFI SMDKDLGDGS TLRSRALEAV SMTRFVPSSG QSRLASMIAD 500
    RPDWVLSRQR AWGVPICIFA NEDGVILKDE RVNERILRAF EAEGADAWFA 550
    EGARERFLGE RAHESWVQVL DILDVWFDSG ASHSFVLEDR DDLKWPADVY 600
    FEGSDQHRGW FQSSLLESCG TRACSPYKAV ITHGFTLDEN GKKMSKSLGN 650
    TVVPQEIIKT SGADIFRLWV MTTDYWEDQR LGKQILQTNM DLYRKLRNAI 700
    RWMLGILAHD EGEKISYCAL PDLEKFILHQ LFELDQLINR AYDEFDFKKI 750
    MRALLDFSIT ELSAFYFDIR KDSLYCDAPS SKKRKASLQV IREIFERMVI 800
    WLAPMLPFTM EEAWLEHSPK SHSVHLEQFR SVPGEWQNGF LAERWRKVRQ 850
    VRKVVTGALE FERAAKRIGS SLEAAPIVFI SNPVLLEALE NLDMAEICIT 900
    SALTITQDVP PSDAFILSDV EGVGVYPGKA LGTKCARSWR YTQDVGSDPA 950
    YPDVSARDAA ALRELQMLGK I 971
    Length:971
    Mass (Da):110,443
    Last modified:July 19, 2004 - v1
    Checksum:iC7478D6326045340
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX897700 Genomic DNA. Translation: CAF25747.1.
    RefSeqiYP_031948.1. NC_005955.1.

    Genome annotation databases

    EnsemblBacteriaiCAF25747; CAF25747; BQ02440.
    GeneIDi2866644.
    KEGGibqu:BQ02440.
    PATRICi31951693. VBIBarQui58630_0286.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX897700 Genomic DNA. Translation: CAF25747.1 .
    RefSeqi YP_031948.1. NC_005955.1.

    3D structure databases

    ProteinModelPortali Q6G0K6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 283165.BQ02440.

    Proteomic databases

    PRIDEi Q6G0K6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAF25747 ; CAF25747 ; BQ02440 .
    GeneIDi 2866644.
    KEGGi bqu:BQ02440.
    PATRICi 31951693. VBIBarQui58630_0286.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci BQUI283165:GHZA-244-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Toulouse.

    Entry informationi

    Entry nameiSYI_BARQU
    AccessioniPrimary (citable) accession number: Q6G0K6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: July 19, 2004
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3