Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Bartonella quintana (strain Toulouse) (Rochalimaea quintana)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei602Aminoacyl-adenylateUniRule annotation1
Binding sitei646ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBQUI283165:G1GJ6-250-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BQ02440
OrganismiBartonella quintana (strain Toulouse) (Rochalimaea quintana)
Taxonomic identifieri283165 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBartonellaceaeBartonella
Proteomesi
  • UP000000597 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000983541 – 971Isoleucine--tRNA ligaseAdd BLAST971

Proteomic databases

PRIDEiQ6G0K6

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi283165.BQ02440

Structurei

3D structure databases

ProteinModelPortaliQ6G0K6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi64 – 74"HIGH" regionAdd BLAST11
Motifi643 – 647"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q6G0K6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVKNETIDY SKTLYLPQTN FPMRGRLPQK ELELIARWDN MGLYAQLRQQ
60 70 80 90 100
AKDRPLYILH DGPPYANGHI HIGHALNKVL KDVIIRSFQM RGFNANYVPG
110 120 130 140 150
WDCHGLPIEW KIEEKYRAQG KNKDDVPLNE FRQECREFAK YWITVQSEEF
160 170 180 190 200
KRLGVVGDFN SPYTTMAFHA EARIASELMK FAMSGQIYRG SKPVMWSVVE
210 220 230 240 250
RTALAEAEIE YHDYESEVIW VKFPVLQADS SDLCGAYVVI WTTTPWTIPG
260 270 280 290 300
NRAVSYSSQI SYGVYEVAST QNDFGPQVGE RLLFADALAM SCAEKAKLVL
310 320 330 340 350
KRLRIISADE LKTLFLFHPL KGFAGSYNDK IAMLDGAHVT ENAGTGFVHT
360 370 380 390 400
APSHGREDFE IWNAYKPLLE QFGIDSSIPF PVDDAGFYTK DAPGFGPNRE
410 420 430 440 450
GGAVRVIDDN GKMGDANKEV INALIKADRL FARGRLKHSY PHSWRSKKPV
460 470 480 490 500
IFRNTPQWFI SMDKDLGDGS TLRSRALEAV SMTRFVPSSG QSRLASMIAD
510 520 530 540 550
RPDWVLSRQR AWGVPICIFA NEDGVILKDE RVNERILRAF EAEGADAWFA
560 570 580 590 600
EGARERFLGE RAHESWVQVL DILDVWFDSG ASHSFVLEDR DDLKWPADVY
610 620 630 640 650
FEGSDQHRGW FQSSLLESCG TRACSPYKAV ITHGFTLDEN GKKMSKSLGN
660 670 680 690 700
TVVPQEIIKT SGADIFRLWV MTTDYWEDQR LGKQILQTNM DLYRKLRNAI
710 720 730 740 750
RWMLGILAHD EGEKISYCAL PDLEKFILHQ LFELDQLINR AYDEFDFKKI
760 770 780 790 800
MRALLDFSIT ELSAFYFDIR KDSLYCDAPS SKKRKASLQV IREIFERMVI
810 820 830 840 850
WLAPMLPFTM EEAWLEHSPK SHSVHLEQFR SVPGEWQNGF LAERWRKVRQ
860 870 880 890 900
VRKVVTGALE FERAAKRIGS SLEAAPIVFI SNPVLLEALE NLDMAEICIT
910 920 930 940 950
SALTITQDVP PSDAFILSDV EGVGVYPGKA LGTKCARSWR YTQDVGSDPA
960 970
YPDVSARDAA ALRELQMLGK I
Length:971
Mass (Da):110,443
Last modified:July 19, 2004 - v1
Checksum:iC7478D6326045340
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX897700 Genomic DNA Translation: CAF25747.1
RefSeqiWP_011179057.1, NC_005955.1

Genome annotation databases

EnsemblBacteriaiCAF25747; CAF25747; BQ02440
GeneIDi34145312
KEGGibqu:BQ02440

Similar proteinsi

Entry informationi

Entry nameiSYI_BARQU
AccessioniPrimary (citable) accession number: Q6G0K6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 19, 2004
Last modified: March 28, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health