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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Bartonella quintana (strain Toulouse) (Rochalimaea quintana)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei602 – 6021Aminoacyl-adenylateUniRule annotation
Binding sitei646 – 6461ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBQUI283165:GHZA-244-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BQ02440
OrganismiBartonella quintana (strain Toulouse) (Rochalimaea quintana)
Taxonomic identifieri283165 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBartonellaceaeBartonella
ProteomesiUP000000597 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 971971Isoleucine--tRNA ligasePRO_0000098354Add
BLAST

Proteomic databases

PRIDEiQ6G0K6.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi283165.BQ02440.

Structurei

3D structure databases

ProteinModelPortaliQ6G0K6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi64 – 7411"HIGH" regionAdd
BLAST
Motifi643 – 6475"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6G0K6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVKNETIDY SKTLYLPQTN FPMRGRLPQK ELELIARWDN MGLYAQLRQQ
60 70 80 90 100
AKDRPLYILH DGPPYANGHI HIGHALNKVL KDVIIRSFQM RGFNANYVPG
110 120 130 140 150
WDCHGLPIEW KIEEKYRAQG KNKDDVPLNE FRQECREFAK YWITVQSEEF
160 170 180 190 200
KRLGVVGDFN SPYTTMAFHA EARIASELMK FAMSGQIYRG SKPVMWSVVE
210 220 230 240 250
RTALAEAEIE YHDYESEVIW VKFPVLQADS SDLCGAYVVI WTTTPWTIPG
260 270 280 290 300
NRAVSYSSQI SYGVYEVAST QNDFGPQVGE RLLFADALAM SCAEKAKLVL
310 320 330 340 350
KRLRIISADE LKTLFLFHPL KGFAGSYNDK IAMLDGAHVT ENAGTGFVHT
360 370 380 390 400
APSHGREDFE IWNAYKPLLE QFGIDSSIPF PVDDAGFYTK DAPGFGPNRE
410 420 430 440 450
GGAVRVIDDN GKMGDANKEV INALIKADRL FARGRLKHSY PHSWRSKKPV
460 470 480 490 500
IFRNTPQWFI SMDKDLGDGS TLRSRALEAV SMTRFVPSSG QSRLASMIAD
510 520 530 540 550
RPDWVLSRQR AWGVPICIFA NEDGVILKDE RVNERILRAF EAEGADAWFA
560 570 580 590 600
EGARERFLGE RAHESWVQVL DILDVWFDSG ASHSFVLEDR DDLKWPADVY
610 620 630 640 650
FEGSDQHRGW FQSSLLESCG TRACSPYKAV ITHGFTLDEN GKKMSKSLGN
660 670 680 690 700
TVVPQEIIKT SGADIFRLWV MTTDYWEDQR LGKQILQTNM DLYRKLRNAI
710 720 730 740 750
RWMLGILAHD EGEKISYCAL PDLEKFILHQ LFELDQLINR AYDEFDFKKI
760 770 780 790 800
MRALLDFSIT ELSAFYFDIR KDSLYCDAPS SKKRKASLQV IREIFERMVI
810 820 830 840 850
WLAPMLPFTM EEAWLEHSPK SHSVHLEQFR SVPGEWQNGF LAERWRKVRQ
860 870 880 890 900
VRKVVTGALE FERAAKRIGS SLEAAPIVFI SNPVLLEALE NLDMAEICIT
910 920 930 940 950
SALTITQDVP PSDAFILSDV EGVGVYPGKA LGTKCARSWR YTQDVGSDPA
960 970
YPDVSARDAA ALRELQMLGK I
Length:971
Mass (Da):110,443
Last modified:July 18, 2004 - v1
Checksum:iC7478D6326045340
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX897700 Genomic DNA. Translation: CAF25747.1.
RefSeqiWP_011179057.1. NC_005955.1.
YP_031948.1. NC_005955.1.

Genome annotation databases

EnsemblBacteriaiCAF25747; CAF25747; BQ02440.
KEGGibqu:BQ02440.
PATRICi31951693. VBIBarQui58630_0286.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX897700 Genomic DNA. Translation: CAF25747.1.
RefSeqiWP_011179057.1. NC_005955.1.
YP_031948.1. NC_005955.1.

3D structure databases

ProteinModelPortaliQ6G0K6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi283165.BQ02440.

Proteomic databases

PRIDEiQ6G0K6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAF25747; CAF25747; BQ02440.
KEGGibqu:BQ02440.
PATRICi31951693. VBIBarQui58630_0286.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciBQUI283165:GHZA-244-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Toulouse.

Entry informationi

Entry nameiSYI_BARQU
AccessioniPrimary (citable) accession number: Q6G0K6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 19, 2005
Last sequence update: July 18, 2004
Last modified: March 31, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.