Reviewed, UniProtKB/Swiss-Prot Q6G0G3 (ISPE_BARQU)

Names and origin

Protein names

Recommended name:
    4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
      Short name=CMK
    EC=2.7.1.148
Alternative name(s):
    4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	BQ03230

Organism

Bartonella quintana (Rochalimaea quintana) [Complete proteome] [HAMAP]

Taxonomic identifier

803 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity.
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphorylation Inferred from electronic annotation. Source: InterPro terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000235069
Regions
	Nucleotide binding	106 – 116	11	ATP Potential
Sites
	Active site	20	1	By similarity
	Active site	148	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6G0G3-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 43014CF1B633B526	FASTA	302	33,124
	10 20 30 40 50 60 MMSDVKVSFK GHSYVFTPIK LNLTLHVVGQ RTDGYHLIES LVYFSFSGDC LSYMPCKSNH 70 80 90 100 110 120 FILTGPFADE LVSDTGNLVV RAHDFMCKAF PKCAKPAFFR LVKLLPVASG IGGGSGNAAG 130 140 150 160 170 180 VLSLLRQQWG LDCSCEKLAE MSLVLGADVP MCLFALEYRQ PLFVRGIGQD ITQLKEACSL 190 200 210 220 230 240 AMVLVNHGQQ IATKAVFKAL EKRDHPPLKI DLESLKTVDS LVEALQETRN DLFIPALKIA 250 260 270 280 290 300 PQLSEVLYAL DESGALFSRM SGTGATCFGI FKDKEAAQQA ALFIKLMHPD WFVKPIITLG ML

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Toulouse.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX897700 Genomic DNA. Translation: CAF25823.1.
RefSeq	YP_032011.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2866968.
GenomeReviews	Gene locus BQ03230 in contig BX897700_GR.
KEGG	bqu:BQ03230.
NMPDR	fig|283165.1.peg.281.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6G0G3.
OMA	QETRNDL.
Enzyme and pathway databases
BioCyc	BQUI283165:BQ03230-MON.
BRENDA	2.7.1.148. 292235.
Family and domain databases
HAMAP	MF_00061. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. [Graphical view]
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
TIGRFAMs	TIGR00154. ispE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_BARQU

Accession

Primary (citable) accession number: Q6G0G3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	July 19, 2004
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)