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Protein

Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme

Gene

glnE

Organism
Bartonella quintana (strain Toulouse) (Rochalimaea quintana)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.UniRule annotation

Catalytic activityi

[Glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP.UniRule annotation
ATP + [glutamine synthetase]-L-tyrosine = diphosphate + [glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine.UniRule annotation

Cofactori

Mg2+UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeUniRule annotation
Alternative name(s):
ATP:glutamine synthetase adenylyltransferaseUniRule annotation
ATaseUniRule annotation
Including the following 2 domains:
Glutamine synthetase adenylyl-L-tyrosine phosphorylaseUniRule annotation (EC:2.7.7.89UniRule annotation)
Alternative name(s):
Adenylyl removaseUniRule annotation
Short name:
ARUniRule annotation
Short name:
AT-NUniRule annotation
Glutamine synthetase adenylyl transferaseUniRule annotation (EC:2.7.7.42UniRule annotation)
Alternative name(s):
Adenylyl transferaseUniRule annotation
Short name:
ATUniRule annotation
Short name:
AT-CUniRule annotation
Gene namesi
Name:glnEUniRule annotation
Ordered Locus Names:BQ04000
OrganismiBartonella quintana (strain Toulouse) (Rochalimaea quintana)
Taxonomic identifieri283165 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBartonellaceaeBartonella
Proteomesi
  • UP000000597 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002092291 – 974Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeAdd BLAST974

Interactioni

Protein-protein interaction databases

STRINGi283165.BQ04000.

Structurei

3D structure databases

ProteinModelPortaliQ6G0B0.
SMRiQ6G0B0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 464Adenylyl removaseUniRule annotationAdd BLAST464
Regioni468 – 974Adenylyl transferaseUniRule annotationAdd BLAST507

Sequence similaritiesi

Belongs to the GlnE family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CE6. Bacteria.
COG1391. LUCA.
HOGENOMiHOG000256492.
KOiK00982.
OMAiEFMVQYA.
OrthoDBiPOG091H0675.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 1 hit.
PF03710. GlnE. 2 hits.

Sequencei

Sequence statusi: Complete.

Q6G0B0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNAFLKTHL FPLCPLDESG SQWLKDIEEQ ARKENLESLV SYFSAKGKQV
60 70 80 90 100
DFISAVMTLS PFLREVLIAN PSYLSPLLYV DIETRLSEII DDVALIDKSE
110 120 130 140 150
SINETALMAA LRRKKREAHI LIALADLSGV FTYEISCTWL TRLGEAALGV
160 170 180 190 200
ALRFLLREAH DHGKISLSSR DNPEKDSGLI ILGLGKLGAG ELNYSSDIDL
210 220 230 240 250
IVFIDEMSPH IGDLSESVDV FSKMVRRLIR IIQERTAEGY VFRLDFRLRP
260 270 280 290 300
DPGSTPLALP VRTALRYYEG RGQNWERAAM IKARPVAGDK RAGFKFLKEL
310 320 330 340 350
FPYVWRKYLD YAAIADIHSI KRQIHACKNY GQITAYGHNI KLGRGGIREI
360 370 380 390 400
EFFVQTQQLI AGGRFPQLRG RQTVAMLTEL HTLGWISEKT SDNLIKSYAF
410 420 430 440 450
LRNVEHRIQM LADEQTHLLP NDVSQFTSVA YLMGYQESSS FIRDLLKVLQ
460 470 480 490 500
VVEKHYAALF ENEQELGLEI GNLVFTGEED DPETLITLSC LGFERASDIC
510 520 530 540 550
RIMRTLHCGR YKATQSAEAR ERLTELTPAL LKAFGAIKRA DEAMLRFDSF
560 570 580 590 600
LQGLPSGIQL FSLLQSNPSL LDMLVLIMGA APRLAEIITR KPHVFDGMLD
610 620 630 640 650
PTILSELPTK TYLENRLEYF LEGVIPYEEI LDHLRVFADE QRFLIGIRIL
660 670 680 690 700
NGAITGEKAG FAFTALADLM IAKTLAAVQE EFSRIHGNIK GGRVGILGMG
710 720 730 740 750
KLGSCELTAG SDVDLILVYE HDEDAEISDG EKPLYISQYY TRFTKRLIAA
760 770 780 790 800
LSTLTSQGVL YAVDLRLRPL GNKGPVAVSF EFFRKYYRKE AWIWEYLALT
810 820 830 840 850
RARGIAGDPN FLQNLENEVC AIIALPHDKK DVAKAVREMR TLIEKEKPPE
860 870 880 890 900
NRWDLKMMSG GIMDVEFIAQ FALITHVVAF QIGASTADIL AHLPNSVLNQ
910 920 930 940 950
SCIVDLHRAY SLYTNLNQMI RLCLNDAFDP HDMPPGLSDL LLSSVGEPDL
960 970
LRVEKLIEET AQSVCSIFKQ IMKH
Length:974
Mass (Da):109,697
Last modified:July 19, 2004 - v1
Checksum:iDF42C8AED078562C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX897700 Genomic DNA. Translation: CAF25899.1.
RefSeqiWP_011179188.1. NC_005955.1.

Genome annotation databases

EnsemblBacteriaiCAF25899; CAF25899; BQ04000.
KEGGibqu:BQ04000.
PATRICi31952047. VBIBarQui58630_0459.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX897700 Genomic DNA. Translation: CAF25899.1.
RefSeqiWP_011179188.1. NC_005955.1.

3D structure databases

ProteinModelPortaliQ6G0B0.
SMRiQ6G0B0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi283165.BQ04000.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAF25899; CAF25899; BQ04000.
KEGGibqu:BQ04000.
PATRICi31952047. VBIBarQui58630_0459.

Phylogenomic databases

eggNOGiENOG4105CE6. Bacteria.
COG1391. LUCA.
HOGENOMiHOG000256492.
KOiK00982.
OMAiEFMVQYA.
OrthoDBiPOG091H0675.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 1 hit.
PF03710. GlnE. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGLNE_BARQU
AccessioniPrimary (citable) accession number: Q6G0B0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: July 19, 2004
Last modified: May 10, 2017
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.