Reviewed, UniProtKB/Swiss-Prot Q6FZU5 (LEXA_BARQU)

Names and origin

Protein names

Recommended name:
    LexA repressor
    EC=3.4.21.88

Gene names

Name:	lexA
Ordered Locus Names:	BQ06160

Organism

Bartonella quintana (Rochalimaea quintana) [Complete proteome] [HAMAP]

Taxonomic identifier

803 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity. HAMAP MF_00015
Catalytic activity	Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015
Subunit structure	Homodimer By similarity. HAMAP MF_00015
Sequence similarities	Belongs to the peptidase S24 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Hydrolase Repressor
PTM	Autocatalytic cleavage
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP proteolysis Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 237	237	LexA repressor HAMAP MF_00015		PRO_0000170012
Regions
	DNA binding	26 – 46	21	H-T-H motif By similarity
Sites
	Active site	158	1	For autocatalytic cleavage activity By similarity
	Active site	196	1	For autocatalytic cleavage activity By similarity
	Site	123 – 124	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6FZU5-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 0A152E6FF49A8299	FASTA	237	26,908
	10 20 30 40 50 60 MLTCKQYELL LFIHNHMKEI GVPPSFDEMK IALELTSKSG IHRLITALEE RGFIRRLPNR 70 80 90 100 110 120 ARAVEVVRLP EKITFNLSSA RKISPNVIEN NRRKISKNSK NLNNFDIEDK KNVTVPIMGR 130 140 150 160 170 180 IAAGVPVSAI QQQTNTLCLP ADMISLGEHY ALEVKDDSMI EAGILDKDTI IVRRQNTATP 190 200 210 220 230 GEIIIALIDK EEATLKRYRR NGASIALEAA NPHYETRIYR PERIEIQGKL IGLIRKY

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Toulouse.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX897700 Genomic DNA. Translation: CAF26107.1.
RefSeq	YP_032265.1.
3D structure databases
SMR	Q6FZU5. Positions 115-237.
ModBase	Search...
Protein family/group databases
MEROPS	S24.001.
Genome annotation databases
GeneID	2866428.
GenomeReviews	Gene locus BQ06160 in contig BX897700_GR.
KEGG	bqu:BQ06160.
NMPDR	fig|283165.1.peg.535.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG679610.
OMA	AILTFIR.
Enzyme and pathway databases
BioCyc	BQUI283165:BQ06160-MONOMER.
BRENDA	3.4.21.88. 292235.
Family and domain databases
HAMAP	MF_00015. LexA. [Tree]
InterPro	IPR006199. LexA_DNA-bd_dom. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA_cons-reg. IPR019759. Peptidase_S24_S26_cons-reg. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00726. LEXASERPTASE.
TIGRFAMs	TIGR00498. lexA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEXA_BARQU

Accession

Primary (citable) accession number: Q6FZU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	July 19, 2004
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)