Reviewed, UniProtKB/Swiss-Prot Q6FZP6 (SYE1_BARQU)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 1
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 1
      Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	BQ06770

Organism

Bartonella quintana (Rochalimaea quintana) [Complete proteome] [HAMAP]

Taxonomic identifier

803 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity. HAMAP MF_00022
Subcellular location	Cytoplasm HAMAP MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 459	459	Glutamyl-tRNA synthetase 1 HAMAP MF_00022		PRO_0000119512
Regions
	Motif	8 – 18	11	"HIGH" region HAMAP MF_00022
	Motif	249 – 253	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	252	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6FZP6-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: B2E0734CD0F05C2E	FASTA	459	52,789
	10 20 30 40 50 60 MIKVRFAPSP TGYIHIGNIR IALFNWLYAQ AHNGAFILRY DDTDVERSKQ EYIDAIAADL 70 80 90 100 110 120 EWLDIQPDEI YYQSKRFNRY DEVAEMLKQR GLLYPCYETA EELDRRRKIQ LSRKLPPVYD 130 140 150 160 170 180 RAALKLTPEE KEEFESQGRK PHWRFLLPNF ENNPLQTKRT EVCWNDVVKG KQTIDLASLS 190 200 210 220 230 240 DPVLIREGGS YLYTLPSVVD DIDMAITHII RGDDHITNTG VQIALFEALN AQLPIFGHIN 250 260 270 280 290 300 LLTTVLGKGL SKRDNDLSIH SLRAEGFESI AIQCFAVLIG TSQNVHPYPH QAALLKHFNL 310 320 330 340 350 360 QDTSRSVTKF DIADLFALNS HLVHDLTYEE VKTRLKNLSI DGEKAECFWN AIRSNIDKVN 370 380 390 400 410 420 DAVLWWKMIH DEQSFDPVAL EDRSFVRQSL NFLPEGPLND ESWKVWTTTL KEKTGRRGKA 430 440 450 LFMPLRQALT GMDHGPEMGK LLQLLGREKV IDRLTIQGE

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Toulouse.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX897700 Genomic DNA. Translation: CAF26166.1.
RefSeq	YP_032314.1.
3D structure databases
SMR	Q6FZP6. Positions 2-455.
ModBase	Search...
Genome annotation databases
GeneID	2867044.
GenomeReviews	Gene locus BQ06770 in contig BX897700_GR.
KEGG	bqu:BQ06770.
NMPDR	fig|283165.1.peg.584.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	LRLDDTD.
Enzyme and pathway databases
BioCyc	BQUI283165:BQ06770-MONOMER.
BRENDA	6.1.1.17. 292235.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_BARQU

Accession

Primary (citable) accession number: Q6FZP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	July 19, 2004
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)