Reviewed, UniProtKB/Swiss-Prot Q6FZ74 (DDL_BARQU)

Names and origin

Protein names

Recommended name:
    D-alanine--D-alanine ligase
    EC=6.3.2.4
Alternative name(s):
    D-alanylalanine synthetase
    D-Ala-D-Ala ligase

Gene names

Name:	ddl
Ordered Locus Names:	BQ08830

Organism

Bartonella quintana (Rochalimaea quintana) [Complete proteome] [HAMAP]

Taxonomic identifier

803 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	D-alanine--D-alanine ligase		PRO_1000030430
Regions
	Domain	102 – 300	199	ATP-grasp
	Nucleotide binding	128 – 183	56	ATP By similarity
Sites
	Metal binding	253	1	Magnesium or manganese 1 By similarity
	Metal binding	267	1	Magnesium or manganese 1 By similarity
	Metal binding	267	1	Magnesium or manganese 2 By similarity
	Metal binding	269	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6FZ74-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 1ACFFD24DB7374BD	FASTA	306	33,288
	10 20 30 40 50 60 MKGEHVAVLM GGGSSERSVS LSSGTACADI LEARGYRVSR VDVDSHIASV LERLQPDVAF 70 80 90 100 110 120 NALHGPFGED GCIQGVLEYL KIPYTHSGVM ASALAMDKGR AKIVVASVGV SVAPSCIMSR 130 140 150 160 170 180 FAVGREHPME PPYVIKPLCE GSSFGVVIVK ENESAPPRNV VGAEWVYADE VIVEKYIPGR 190 200 210 220 230 240 EFTCAVLGNE ALDVCEIFPD KHFQFYNYDS KYKSGGSLHI CPAQLSPNIY QNVQRMSLAA 250 260 270 280 290 300 HQAIGCRGVS RSDFRFNEET GELVWLEINT QPGMTPTSLF PDIAKASGRT YGDIVQWMVE DASCMR

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Toulouse.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX897700 Genomic DNA. Translation: CAF26362.1.
RefSeq	YP_032495.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2867175.
GenomeReviews	Gene locus BQ08830 in contig BX897700_GR.
KEGG	bqu:BQ08830.
NMPDR	fig|283165.1.peg.765.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6FZ74.
Enzyme and pathway databases
BioCyc	BQUI283165:BQ08830-MON.
Family and domain databases
HAMAP	MF_00047. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDL_BARQU

Accession

Primary (citable) accession number: Q6FZ74

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 19, 2004
Last modified:	November 25, 2008
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)