Q6FYH4 (TRMD_BARQU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	BQ12740

Organism

Bartonella quintana (strain Toulouse) (Rochalimaea quintana) [Complete proteome] [HAMAP]

Taxonomic identifier

283165 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm Potential HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 232	232	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000060332
Regions
	Region	131 – 136	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	111	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6FYH4 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: DCE7E3335D163B3F	FASTA	232	25,707
	10 20 30 40 50 60 MKFQARVLTL YPEMFPGFLG YSLAGQALER GIWSLETVQI RDFALDKHHS VDDTPAGGGA 70 80 90 100 110 120 GMVMRADVLA AALDSCPHDS TRLLMSPRGR PFNQAYARFL ACESGVTLVC GRFEGVDERI 130 140 150 160 170 180 IEARKLEEVS IGDYILSGGE TAALVLLDAI VRLLPGVMGN EASGKCESFE NGLLEHPQYT 190 200 210 220 230 RPSIFEGRCI PPVLTSGHHK AIADWRQQQA ELLTRQRRPE LYALYDKNRQ KT

References

[1]

"The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae." Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H., Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M., La Scola B., Holmberg M., Andersson S.G.E. Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004) [PubMed: 15210978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Toulouse.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX897700 Genomic DNA. Translation: CAF26733.1.
RefSeq	YP_032801.1. NC_005955.1.
3D structure databases
ProteinModelPortal	Q6FYH4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2866558.
GenomeReviews	Gene locus BQ12740 in contig BX897700_GR.
KEGG	bqu:BQ12740.
NMPDR	fig|283165.1.peg.1071.
PATRIC	31953991. VBIBarQui58630_1393.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285805.
OMA	VCGRFEG.
PhylomeDB	Q6FYH4.
ProtClustDB	PRK00026.
Enzyme and pathway databases
BioCyc	BQUI283165:BQ12740-MONOMER.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. TrmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_BARQU

Accession

Primary (citable) accession number: Q6FYH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program