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Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

tsaD

Organism
Bartonella quintana (strain Toulouse) (Rochalimaea quintana)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi115 – 1151IronUniRule annotation
Metal bindingi119 – 1191IronUniRule annotation
Binding sitei170 – 1701SubstrateUniRule annotation
Binding sitei183 – 1831Substrate; via amide nitrogenUniRule annotation
Binding sitei288 – 2881SubstrateUniRule annotation
Metal bindingi316 – 3161IronUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciBQUI283165:GHZA-1299-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
Gene namesi
Name:tsaDUniRule annotation
Synonyms:gcp
Ordered Locus Names:BQ13010
OrganismiBartonella quintana (strain Toulouse) (Rochalimaea quintana)
Taxonomic identifieri283165 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBartonellaceaeBartonella
Proteomesi
  • UP000000597 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 364364tRNA N6-adenosine threonylcarbamoyltransferasePRO_0000303277Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi283165.BQ13010.

Structurei

3D structure databases

ProteinModelPortaliQ6FYF1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni137 – 1415Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPM. Bacteria.
COG0533. LUCA.
HOGENOMiHOG000109568.
KOiK01409.
OMAiRDHVKRM.
OrthoDBiPOG091H010B.

Family and domain databases

HAMAPiMF_01445. TsaD. 1 hit.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/TsaD.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_TsaD_YgjD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6FYF1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLLGIETSC DETAAAVIEH NIEGNSQILS NIVWSQTDNH APYGGVVPEI
60 70 80 90 100
AARAHVEILD DLILKALTDA HTKLKDIDAI AVTSGPGLIG GLLVGVMSAK
110 120 130 140 150
ALSLATGKPF IAVNHLEGHA LTAVLTHNVA FPYLLLLVSG GHTQTILVHE
160 170 180 190 200
VGNYQRLGTT IDDALGEAFD KTAKLLGLPY PGGPALEKAA LLGDKNRISL
210 220 230 240 250
PRPLKGEKRL DFSFSGLKTA VRQAATAMSP LTENDVADIA ASFQAAVTDT
260 270 280 290 300
VYDRVHLALQ YFTHQYPLSH YKGRRPPALV VAGGVAANQA IRLTLQELAH
310 320 330 340 350
QHGFEFIAPP LSLCTDNAAM IAFAGAQKFA RGEKNSFDIA PRSRWPLDEK
360
AAPLIGTGRR GTKA
Length:364
Mass (Da):38,745
Last modified:July 19, 2004 - v1
Checksum:i0A474F5692054300
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX897700 Genomic DNA. Translation: CAF26760.1.
RefSeqiWP_011179922.1. NC_005955.1.

Genome annotation databases

EnsemblBacteriaiCAF26760; CAF26760; BQ13010.
KEGGibqu:BQ13010.
PATRICi31954047. VBIBarQui58630_1421.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX897700 Genomic DNA. Translation: CAF26760.1.
RefSeqiWP_011179922.1. NC_005955.1.

3D structure databases

ProteinModelPortaliQ6FYF1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi283165.BQ13010.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAF26760; CAF26760; BQ13010.
KEGGibqu:BQ13010.
PATRICi31954047. VBIBarQui58630_1421.

Phylogenomic databases

eggNOGiENOG4105CPM. Bacteria.
COG0533. LUCA.
HOGENOMiHOG000109568.
KOiK01409.
OMAiRDHVKRM.
OrthoDBiPOG091H010B.

Enzyme and pathway databases

BioCyciBQUI283165:GHZA-1299-MONOMER.

Family and domain databases

HAMAPiMF_01445. TsaD. 1 hit.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/TsaD.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_TsaD_YgjD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTSAD_BARQU
AccessioniPrimary (citable) accession number: Q6FYF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 19, 2004
Last modified: September 7, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.