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Q6FVD8 (DNLI4_CANGA) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA ligase 4

EC=6.5.1.1
Alternative name(s):
DNA ligase IV
Polydeoxyribonucleotide synthase [ATP] 4
Gene names
Name:LIG4
Ordered Locus Names:CAGL0E02695g
OrganismCandida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata) [Complete proteome]
Taxonomic identifier284593 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeNakaseomycesmitosporic Nakaseomyces

Protein attributes

Sequence length946 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining By similarity.

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactor

Magnesium By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Contains 2 BRCT domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 946946DNA ligase 4
PRO_0000278378

Regions

Domain688 – 787100BRCT 1
Domain845 – 945101BRCT 2

Sites

Active site2971N6-AMP-lysine intermediate By similarity
Metal binding3551Magnesium 1 Potential
Metal binding4571Magnesium 2 Potential
Binding site2951ATP By similarity
Binding site3021ATP By similarity
Binding site3191ATP By similarity
Binding site4621ATP By similarity
Binding site4731ATP By similarity
Binding site4791ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6FVD8 [UniParc].

Last modified July 19, 2004. Version 1.
Checksum: F6AC2CD321B55EEA

FASTA946109,554
        10         20         30         40         50         60 
MADEGGLETG AHDELKGTEE QAVNFAPSPD FLWLCEQLFA KIDHVQFERA NNLLTKPVTA 

        70         80         90        100        110        120 
RYYEVISNFT TLWRTTVGNN IYPALRLILP YRDRRVFNIK DYTLIKAICA FLKLPKDSST 

       130        140        150        160        170        180 
EKKLINWKQD AGRSVRLSKF CVEEIKKRRS EPQIDRNERI TIDDLNGYLD QLAIERTEQG 

       190        200        210        220        230        240 
RSFKNLANSD IMNKCLTSMT FLEMQYFFDI LLKNRPLGGH EHKLLNCWHP DAQDYLSVVS 

       250        260        270        280        290        300 
DLETVAKRLW DPSQRLGNQD LKINIGLAFA PQLATKLHVS YQKIGEKLGW DFFIEEKMDG 

       310        320        330        340        350        360 
ERIQMHYTNF GSDIKFYSRR ATDYTYLYGN NLKTGTLANF INLNKNVKDC VLDCEVVTFD 

       370        380        390        400        410        420 
SNNKIVLPFG MVKSSAKNML SQDGIDTQGF HPLLMVFDVL YLNGATLVDL PYYKRREYLK 

       430        440        450        460        470        480 
QILTPTAHRI EIIKSIRAND EQMIKKSLEK ALSVGSEGII LKRYDSRYVI ASRSDDWIKI 

       490        500        510        520        530        540 
KPEYLEQFGE NMDLVLMGRD PSKKDSLMLG LLDYEEVIQD SPIMVNSQSS EENSQRFRGF 

       550        560        570        580        590        600 
VSLCIIANGI SNEEYKEIDR KTKGLWNDSE KIPPLEYMKF GSKVPRQWID PKKSLILEIK 

       610        620        630        640        650        660 
ARSLDNTRSS ERKFAAGCTL FGGYCRQIRE DKNWKTCYTL QEFERAKSGN NWRKRGSSKP 

       670        680        690        700        710        720 
QKVISKKRRY NIISSVNKAL EDFAELEHRS DIFDGMYFYV LSDYFDGVKR KRIKKSEIQK 

       730        740        750        760        770        780 
VIVANGGQLV QNVITRNYNL NDLRIISSRN TVECNSLIVR GYDIISPKWV FDCLLSGKIM 

       790        800        810        820        830        840 
KLEPSHCFNF SKQLMDYAYK RIDQYGDPYE RDINKYEWSS LTSEKICTTA KQQPDVQFDN 

       850        860        870        880        890        900 
SLMDVPHFLF HGRIVFLLSD NNNIQKESFM VDAYGGKVTN ELSSANLVIV VGAVTQRRIN 

       910        920        930        940 
DIRKQISSEV IKQDHPPRIP DMVSEGWLYD CIKQNTQVAE DNYRLP 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR380951 Genomic DNA. Translation: CAG58725.1.
RefSeqXP_445806.1. XM_445806.1.

3D structure databases

HSSPHSSP built from PDB template 1Z56 based on UniProtKB Q08387.
ProteinModelPortalQ6FVD8.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2887446.
GenomeReviewsGene locus CAGL0E02695g in contig CR380951_GR.
KEGGcgr:CAGL0E02695g.

Phylogenomic databases

eggNOGfuNOG06512.
HOGENOMHBG396164.
OMACESREQK.
OrthoDBEOG41K2MD.

Family and domain databases

InterProIPR001357. BRCT.
IPR000977. DNA_ligase_ATP-dep.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KOK10777.
PfamPF00533. BRCT. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SMARTSM00292. BRCT. 2 hits.
[Graphical view]
SUPFAMSSF52113. BRCT. 2 hits.
SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMsTIGR00574. Dnl1. 1 hit.
PROSITEPS50172. BRCT. 2 hits.
PS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI4_CANGA
AccessionPrimary (citable) accession number: Q6FVD8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: July 19, 2004
Last modified: December 14, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families