DNA ligase 4 - Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast)

Preferred order of sections

Names and origin

Protein names

Recommended name:
DNA ligase 4
EC=6.5.1.1

Alternative name(s):
DNA ligase IV
Polydeoxyribonucleotide synthase [ATP] 4

Gene names

Name:	LIG4
Ordered Locus Names:	CAGL0E02695g

Organism

Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata) [Complete proteome]

Taxonomic identifier

284593 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Nakaseomyces › mitosporic Nakaseomyces

Protein attributes

Sequence length	946 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Cofactor	Magnesium By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family. Contains 2 BRCT domains.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair DNA replication
Cellular component	Nucleus
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA ligation involved in DNA repair Inferred from electronic annotation. Source: InterPro DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 946

946

DNA ligase 4

PRO_0000278378

Regions

Domain

688 – 787

100

BRCT 1

Domain

845 – 945

101

BRCT 2

Sites

Active site

297

1

N6-AMP-lysine intermediate By similarity

Metal binding

355

1

Magnesium 1 Potential

Metal binding

457

1

Magnesium 2 Potential

Binding site

295

1

ATP By similarity

Binding site

302

1

ATP By similarity

Binding site

319

1

ATP By similarity

Binding site

462

1

ATP By similarity

Binding site

473

1

ATP By similarity

Binding site

479

1

ATP By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q6FVD8 [UniParc].

Last modified July 19, 2004. Version 1.
Checksum: F6AC2CD321B55EEA
Show »

FASTA

946

109,554

        10         20         30         40         50         60 
MADEGGLETG AHDELKGTEE QAVNFAPSPD FLWLCEQLFA KIDHVQFERA NNLLTKPVTA 

        70         80         90        100        110        120 
RYYEVISNFT TLWRTTVGNN IYPALRLILP YRDRRVFNIK DYTLIKAICA FLKLPKDSST 

       130        140        150        160        170        180 
EKKLINWKQD AGRSVRLSKF CVEEIKKRRS EPQIDRNERI TIDDLNGYLD QLAIERTEQG 

       190        200        210        220        230        240 
RSFKNLANSD IMNKCLTSMT FLEMQYFFDI LLKNRPLGGH EHKLLNCWHP DAQDYLSVVS 

       250        260        270        280        290        300 
DLETVAKRLW DPSQRLGNQD LKINIGLAFA PQLATKLHVS YQKIGEKLGW DFFIEEKMDG 

       310        320        330        340        350        360 
ERIQMHYTNF GSDIKFYSRR ATDYTYLYGN NLKTGTLANF INLNKNVKDC VLDCEVVTFD 

       370        380        390        400        410        420 
SNNKIVLPFG MVKSSAKNML SQDGIDTQGF HPLLMVFDVL YLNGATLVDL PYYKRREYLK 

       430        440        450        460        470        480 
QILTPTAHRI EIIKSIRAND EQMIKKSLEK ALSVGSEGII LKRYDSRYVI ASRSDDWIKI 

       490        500        510        520        530        540 
KPEYLEQFGE NMDLVLMGRD PSKKDSLMLG LLDYEEVIQD SPIMVNSQSS EENSQRFRGF 

       550        560        570        580        590        600 
VSLCIIANGI SNEEYKEIDR KTKGLWNDSE KIPPLEYMKF GSKVPRQWID PKKSLILEIK 

       610        620        630        640        650        660 
ARSLDNTRSS ERKFAAGCTL FGGYCRQIRE DKNWKTCYTL QEFERAKSGN NWRKRGSSKP 

       670        680        690        700        710        720 
QKVISKKRRY NIISSVNKAL EDFAELEHRS DIFDGMYFYV LSDYFDGVKR KRIKKSEIQK 

       730        740        750        760        770        780 
VIVANGGQLV QNVITRNYNL NDLRIISSRN TVECNSLIVR GYDIISPKWV FDCLLSGKIM 

       790        800        810        820        830        840 
KLEPSHCFNF SKQLMDYAYK RIDQYGDPYE RDINKYEWSS LTSEKICTTA KQQPDVQFDN 

       850        860        870        880        890        900 
SLMDVPHFLF HGRIVFLLSD NNNIQKESFM VDAYGGKVTN ELSSANLVIV VGAVTQRRIN 

       910        920        930        940 
DIRKQISSEV IKQDHPPRIP DMVSEGWLYD CIKQNTQVAE DNYRLP

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References

[1]

"Genome evolution in yeasts."
Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.

Souciet J.-L.
Nature 430:35-44(2004) [PubMed: 15229592] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR380951 Genomic DNA. Translation: CAG58725.1.
RefSeq	XP_445806.1. XM_445806.1.
3D structure databases
HSSP	HSSP built from PDB template 1Z56 based on UniProtKB Q08387.
ProteinModelPortal	Q6FVD8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2887446.
GenomeReviews	Gene locus CAGL0E02695g in contig CR380951_GR.
KEGG	cgr:CAGL0E02695g.
Phylogenomic databases
eggNOG	fuNOG06512.
HOGENOM	HBG396164.
OMA	CESREQK.
OrthoDB	EOG41K2MD.
Family and domain databases
InterPro	IPR001357. BRCT. IPR000977. DNA_ligase_ATP-dep. IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K10777.
Pfam	PF00533. BRCT. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
SMART	SM00292. BRCT. 2 hits. [Graphical view]
SUPFAM	SSF52113. BRCT. 2 hits. SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00574. Dnl1. 1 hit.
PROSITE	PS50172. BRCT. 2 hits. PS00697. DNA_LIGASE_A1. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI4_CANGA

Accession

Primary (citable) accession number: Q6FVD8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	July 19, 2004
Last modified:	December 14, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents