Reviewed, UniProtKB/Swiss-Prot Q6FVD8 (DNLI4_CANGA)

Names and origin

Protein names

Recommended name:
    DNA ligase 4
    EC=6.5.1.1
Alternative name(s):
    DNA ligase IV
    Polydeoxyribonucleotide synthase [ATP] 4

Gene names

Name:	LIG4
Ordered Locus Names:	CAGL0E02695g

Organism

Candida glabrata (Yeast) (Torulopsis glabrata) [Complete proteome]

Taxonomic identifier

5478 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Nakaseomyces › mitosporic Nakaseomyces

Protein attributes

Sequence length	946 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Cofactor	Magnesium By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family. Contains 2 BRCT domains.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair DNA replication
Cellular component	Nucleus
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 946	946	DNA ligase 4		PRO_0000278378
Regions
	Domain	688 – 787	100	BRCT 1
	Domain	845 – 945	101	BRCT 2
Sites
	Active site	297	1	N6-AMP-lysine intermediate By similarity
	Metal binding	355	1	Magnesium 1 Potential
	Metal binding	457	1	Magnesium 2 Potential
	Binding site	295	1	ATP By similarity
	Binding site	302	1	ATP By similarity
	Binding site	319	1	ATP By similarity
	Binding site	462	1	ATP By similarity
	Binding site	473	1	ATP By similarity
	Binding site	479	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6FVD8-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: F6AC2CD321B55EEA	FASTA	946	109,554
	10 20 30 40 50 60 MADEGGLETG AHDELKGTEE QAVNFAPSPD FLWLCEQLFA KIDHVQFERA NNLLTKPVTA 70 80 90 100 110 120 RYYEVISNFT TLWRTTVGNN IYPALRLILP YRDRRVFNIK DYTLIKAICA FLKLPKDSST 130 140 150 160 170 180 EKKLINWKQD AGRSVRLSKF CVEEIKKRRS EPQIDRNERI TIDDLNGYLD QLAIERTEQG 190 200 210 220 230 240 RSFKNLANSD IMNKCLTSMT FLEMQYFFDI LLKNRPLGGH EHKLLNCWHP DAQDYLSVVS 250 260 270 280 290 300 DLETVAKRLW DPSQRLGNQD LKINIGLAFA PQLATKLHVS YQKIGEKLGW DFFIEEKMDG 310 320 330 340 350 360 ERIQMHYTNF GSDIKFYSRR ATDYTYLYGN NLKTGTLANF INLNKNVKDC VLDCEVVTFD 370 380 390 400 410 420 SNNKIVLPFG MVKSSAKNML SQDGIDTQGF HPLLMVFDVL YLNGATLVDL PYYKRREYLK 430 440 450 460 470 480 QILTPTAHRI EIIKSIRAND EQMIKKSLEK ALSVGSEGII LKRYDSRYVI ASRSDDWIKI 490 500 510 520 530 540 KPEYLEQFGE NMDLVLMGRD PSKKDSLMLG LLDYEEVIQD SPIMVNSQSS EENSQRFRGF 550 560 570 580 590 600 VSLCIIANGI SNEEYKEIDR KTKGLWNDSE KIPPLEYMKF GSKVPRQWID PKKSLILEIK 610 620 630 640 650 660 ARSLDNTRSS ERKFAAGCTL FGGYCRQIRE DKNWKTCYTL QEFERAKSGN NWRKRGSSKP 670 680 690 700 710 720 QKVISKKRRY NIISSVNKAL EDFAELEHRS DIFDGMYFYV LSDYFDGVKR KRIKKSEIQK 730 740 750 760 770 780 VIVANGGQLV QNVITRNYNL NDLRIISSRN TVECNSLIVR GYDIISPKWV FDCLLSGKIM 790 800 810 820 830 840 KLEPSHCFNF SKQLMDYAYK RIDQYGDPYE RDINKYEWSS LTSEKICTTA KQQPDVQFDN 850 860 870 880 890 900 SLMDVPHFLF HGRIVFLLSD NNNIQKESFM VDAYGGKVTN ELSSANLVIV VGAVTQRRIN 910 920 930 940 DIRKQISSEV IKQDHPPRIP DMVSEGWLYD CIKQNTQVAE DNYRLP

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 2001 / CBS 138 / IFO 0622 / NRRL Y-65.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR380951 Genomic DNA. Translation: CAG58725.1.
RefSeq	XP_445806.1.
3D structure databases
HSSP	HSSP built from PDB template 1Z56 based on UniProtKB Q08387.
SMR	Q6FVD8. Positions 23-646, 690-943.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6FVD8.
Genome annotation databases
GeneID	2887446.
GenomeReviews	Gene locus CAGL0E02695g in contig CR380951_GR.
KEGG	cgr:CAGL0E02695g.
Phylogenomic databases
eggNOG	fuNOG06512.
HOGENOM	HBG396164.
OMA	ENRDEGI.
OrthoDB	EOG922C2R.
PhylomeDB	Q6FVD8.
Enzyme and pathway databases
BRENDA	6.5.1.1. 189220.
Family and domain databases
InterPro	IPR001357. BRCT. IPR000977. DNA_ligase. IPR012310. DNA_ligase_A_M. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF00533. BRCT. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. [Graphical view]
SMART	SM00292. BRCT. 2 hits. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS50172. BRCT. 2 hits. PS00697. DNA_LIGASE_A1. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI4_CANGA

Accession

Primary (citable) accession number: Q6FVD8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	July 19, 2004
Last modified:	February 9, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)