Q6FUZ2 (CET1_CANGA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
mRNA-capping enzyme subunit beta
EC=3.1.3.33

Alternative name(s):
Polynucleotide 5'-triphosphatase
mRNA 5'-triphosphatase
Short name=TPase

Gene names

Name:	CET1
Ordered Locus Names:	CAGL0E06050g

Organism

Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata) [Complete proteome]

Taxonomic identifier

284593 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Nakaseomyces › mitosporic Nakaseomyces

Protein attributes

Sequence length	602 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	First step of mRNA capping. Converts the 5'-triphosphate end of a nascent mRNA chain into a diphosphate end By similarity.
Catalytic activity	A 5'-phosphopolynucleotide + H2O = a polynucleotide + phosphate.
Cofactor	Divalent ions By similarity.
Subunit structure	The mRNA-capping enzyme is composed of two separate chains alpha and beta, respectively a mRNA guanylyltransferase and an RNA 5'-triphosphatase By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the fungal TPase family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Nucleus
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polynucleotide 5'-phosphatase activity Inferred from electronic annotation. Source: EC transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 602	602	mRNA-capping enzyme subunit beta		PRO_0000210113
Sites
	Active site	276	1	N6-GMP-lysine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6FUZ2 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: A58C2F3412D70C4F	FASTA	602	68,350
	10 20 30 40 50 60 MSEHHSKRAL SLDDLVNHDE NDKSKLQKLA DNESSVRSDD NRPGAIENIV NGNNSNSDLN 70 80 90 100 110 120 SNGVIEEDTD TDDDVGGEFT FDNGITFDYD KQDRFSPEKK RIQARKKDTS KTTPSISNES 130 140 150 160 170 180 PSNSKESSVP VDPLSSNISA TDRKDSSEEK PDLTGPELVK EPDTNEYKRP SIQSITNAED 190 200 210 220 230 240 TTYNDHKAAG MEKTSNKHSL PNILSDSIDE TVTEEHKPKT ETEQTITEYQ QENKQKDNVN 250 260 270 280 290 300 ESNSEETHDI KNDNMNQVEK IFQEKTSTLS KKNSVKKDLE LLNEISASSK PNKYKNTPIW 310 320 330 340 350 360 AQKWKPTVKA LQNIDTNDFK IDNSILDIIP DDDLTKSVQD WVYATLYSID PDLRPFIELE 370 380 390 400 410 420 MKFGVLLESK SPDRVNPPVS SQAVYTDMDA HLTPNVDETV FKELSKYIQS LSEITENAGK 430 440 450 460 470 480 FNVIEAQTKD AVYRVGTSTQ RPRFLRMSSD VKTGRIGAFI EKRHISQLLI YSPKDSYDVK 490 500 510 520 530 540 LSINLELPVP ENDPPEKYQH QTPVSERTKE RVSYIHNDSC TRFDITKVQN HNKGIKSNDV 550 560 570 580 590 600 EITHEIELEI NTPALIKAFD NIMTDSKEYA TLIRTFLNNG TIVRRKLSSL SYEIFEGQKK IQ

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR380951 Genomic DNA. Translation: CAG58871.1.
RefSeq	XP_445952.1. XM_445952.1.
3D structure databases
ProteinModelPortal	Q6FUZ2.
SMR	Q6FUZ2. Positions 294-592.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2887417.
GenomeReviews	Gene locus CAGL0E06050g in contig CR380951_GR.
KEGG	cgr:CAGL0E06050g.
Phylogenomic databases
eggNOG	fuNOG08284.
OMA	VEITHEI.
OrthoDB	EOG4N605N.
Family and domain databases
InterPro	IPR023577. CYTH-like_domain. IPR004206. mRNA_capping_enz_bsu_dom. [Graphical view]
Gene3D	G3DSA:3.20.100.10. mRNA_capping_enz_bsu. 1 hit.
KO	K01098.
Pfam	PF02940. mRNA_triPase. 1 hit. [Graphical view]
SUPFAM	SSF55154. mRNA_capping_enz_bsu. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CET1_CANGA

Accession

Primary (citable) accession number: Q6FUZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	July 19, 2004
Last modified:	December 14, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program