Q6FUX5 (MCR1_CANGA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-cytochrome b5 reductase 2
EC=1.6.2.2

Alternative name(s):
Mitochondrial cytochrome b reductase

Gene names

Name:	MCR1
Ordered Locus Names:	CAGL0E06424g

Organism

Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata) [Complete proteome]

Taxonomic identifier

284593 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Nakaseomyces › mitosporic Nakaseomyces

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May mediate the reduction of outer membrane cytochrome b5 By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane Transmembrane helix
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 298	298	NADH-cytochrome b5 reductase 2		PRO_0000330177
Regions
	Transmembrane	13 – 33	21	Helical; Potential
	Domain	48 – 152	105	FAD-binding FR-type
	Nucleotide binding	155 – 190	36	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6FUX5 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 76B38A123EEB1C7A	FASTA	298	33,610
	10 20 30 40 50 60 MAFARFARPT QRFLPFAIGA VAVTAGALYL NGWNTIKNEN PKVFIGDRKW IDLELEKIIE 70 80 90 100 110 120 ESHDTKRFFF KLPTDDSVSG LTLASAVLTK FMTPKGNPVI RPYTPVSDLS EKGYIEFVIK 130 140 150 160 170 180 HYEGGKMTDH LFQLKPKDTL AFQGPIPKWQ WKPNSFDTIT LLGGGTGITP LYQLVHHITQ 190 200 210 220 230 240 NKEDKTKINL FYGSKTPSDI LLKKELDDLQ KKYPEQLNIQ YFVDKDDTGK FDGNKGFITK 250 260 270 280 290 DFLAKNAPGP KEKTQVFVCG PPPFMDSLSG QKKSPMEQGD LTGALKDLGY SQDQVFKF

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR380951 Genomic DNA. Translation: CAG58888.1.
RefSeq	XP_445969.1. XM_445969.1.
3D structure databases
HSSP	HSSP built from PDB template 1QX4 based on UniProtKB P20070.
ProteinModelPortal	Q6FUX5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2887376.
GenomeReviews	Gene locus CAGL0E06424g in contig CR380951_GR.
KEGG	cgr:CAGL0E06424g.
Phylogenomic databases
eggNOG	fuNOG07850.
HOGENOM	HBG591994.
OMA	RYSPNMK.
OrthoDB	EOG4FFH9V.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD-bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
KO	K00326.
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCR1_CANGA

Accession

Primary (citable) accession number: Q6FUX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	July 19, 2004
Last modified:	December 14, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program