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Protein

Deoxyhypusine hydroxylase

Gene

LIA1

Organism
Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.UniRule annotation

Catalytic activityi

[eIF5A]-deoxyhypusine + AH2 + O2 = [eIF5A]-hypusine + A + H2O.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 2 Fe2+ ions per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi78 – 781Iron 1UniRule annotation
Metal bindingi79 – 791Iron 1UniRule annotation
Metal bindingi111 – 1111Iron 1UniRule annotation
Metal bindingi112 – 1121Iron 1UniRule annotation
Metal bindingi236 – 2361Iron 2UniRule annotation
Metal bindingi237 – 2371Iron 2UniRule annotation
Metal bindingi269 – 2691Iron 2UniRule annotation
Metal bindingi270 – 2701Iron 2UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Hypusine biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine hydroxylaseUniRule annotation (EC:1.14.99.29UniRule annotation)
Short name:
DOHHUniRule annotation
Alternative name(s):
Deoxyhypusine dioxygenaseUniRule annotation
Deoxyhypusine monooxygenaseUniRule annotation
Gene namesi
Name:LIA1UniRule annotation
Ordered Locus Names:CAGL0F00407g
OrganismiCandida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
Taxonomic identifieri284593 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeNakaseomycesNakaseomyces/Candida clade
ProteomesiUP000002428 Componenti: Chromosome F

Organism-specific databases

EuPathDBiFungiDB:CAGL0F00407g.

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Nucleus UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 322322Deoxyhypusine hydroxylasePRO_0000283659Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ6FUV3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati109 – 13527HEAT-like PBS-type 1Add
BLAST
Repeati203 – 22927HEAT-like PBS-type 2Add
BLAST
Repeati234 – 26027HEAT-like PBS-type 3Add
BLAST
Repeati267 – 29327HEAT-like PBS-type 4Add
BLAST

Sequence similaritiesi

Belongs to the deoxyhypusine hydroxylase family.UniRule annotation
Contains 4 HEAT-like PBS-type repeats.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000248665.
InParanoidiQ6FUV3.
KOiK06072.
OMAiDRIIWEN.
OrthoDBiEOG7QC85Z.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
HAMAPiMF_03101. Deoxyhypusine_hydroxylase.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR027517. Deoxyhypusine_hydroxylase.
IPR004155. PBS_lyase_HEAT.
[Graphical view]
SMARTiSM00567. EZ_HEAT. 5 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6FUV3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTNFEKHFE ENVDDCNLEQ LRDILVNKEG KSALANRFRA LFNLKTAASE
60 70 80 90 100
FEANPSDAEK AVQYMGETFG DNSELLKHEV AYVLGQTKNL KAAPLLRKTM
110 120 130 140 150
LDLAQQPMVR HEAAEALGAL GDKDSLEDLE KCLKNDPHVA VRETCELAIA
160 170 180 190 200
RINWQHSDAP TKESLQQSLY SSIDPAPPLA LEKEYDLEEL KKLLNDQEKP
210 220 230 240 250
LFLRYRAMFR LRDIGTDEAV LALASGFNDP SALFKHEIAY VFGQMGSTAA
260 270 280 290 300
VPSLTEVLGR KEEAPMVRHE AAEALGAIAS EDALPILKQY LNDEVDVVRE
310 320
SAIVALDMWE YENSNELEYA PA
Length:322
Mass (Da):35,970
Last modified:July 19, 2004 - v1
Checksum:i45E5380F9C7D3AC2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR380952 Genomic DNA. Translation: CAG58915.1.
RefSeqiXP_445991.1. XM_445991.1.

Genome annotation databases

GeneIDi2887799.
KEGGicgr:CAGL0F00407g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR380952 Genomic DNA. Translation: CAG58915.1.
RefSeqiXP_445991.1. XM_445991.1.

3D structure databases

ProteinModelPortaliQ6FUV3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2887799.
KEGGicgr:CAGL0F00407g.

Organism-specific databases

EuPathDBiFungiDB:CAGL0F00407g.

Phylogenomic databases

HOGENOMiHOG000248665.
InParanoidiQ6FUV3.
KOiK06072.
OMAiDRIIWEN.
OrthoDBiEOG7QC85Z.

Enzyme and pathway databases

UniPathwayiUPA00354.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
HAMAPiMF_03101. Deoxyhypusine_hydroxylase.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR027517. Deoxyhypusine_hydroxylase.
IPR004155. PBS_lyase_HEAT.
[Graphical view]
SMARTiSM00567. EZ_HEAT. 5 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDOHH_CANGA
AccessioniPrimary (citable) accession number: Q6FUV3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: July 19, 2004
Last modified: April 29, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.