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Q6FUV3 (DOHH_CANGA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Deoxyhypusine hydroxylase
Short name=DOHH
EC=1.14.99.29
Alternative name(s):
Deoxyhypusine dioxygenase
Deoxyhypusine monooxygenase
Gene names
Name:LIA1
Ordered Locus Names:CAGL0F00407g
OrganismCandida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata) [Complete proteome]
Taxonomic identifier284593 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Nakaseomyces › mitosporic Nakaseomyces ›

Protein attributes

Sequence length322 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor By similarity. HAMAP-Rule MF_03101

Catalytic activity

Protein N(6)-(4-aminobutyl)-L-lysine + AH2 + O2 = protein N(6)-((R)-4-amino-2-hydroxybutyl)-L-lysine + A + H2O. HAMAP-Rule MF_03101

Cofactor

Binds 2 Fe2+ ions per subunit By similarity. HAMAP-Rule MF_03101

Pathway

Protein modification; eIF5A hypusination. HAMAP-Rule MF_03101

Subcellular location

Cytoplasm. Nucleus By similarity HAMAP-Rule MF_03101.

Sequence similarities

Belongs to the deoxyhypusine hydroxylase family.

Contains 4 HEAT-like PBS-type repeats.

Ontologies

Keywords
   Biological processHypusine biosynthesis
   Cellular componentCytoplasm
Nucleus
   DomainRepeat
   LigandIron
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processpeptidyl-lysine modification to hypusine

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiondeoxyhypusine monooxygenase activity

Inferred from electronic annotation. Source: EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 322322Deoxyhypusine hydroxylase HAMAP-Rule MF_03101
PRO_0000283659

Regions

Repeat109 – 13527HEAT-like PBS-type 1 HAMAP-Rule MF_03101
Repeat203 – 22927HEAT-like PBS-type 2 HAMAP-Rule MF_03101
Repeat234 – 26027HEAT-like PBS-type 3 HAMAP-Rule MF_03101
Repeat267 – 29327HEAT-like PBS-type 4 HAMAP-Rule MF_03101

Sites

Metal binding781Iron 1 By similarity
Metal binding791Iron 1 By similarity
Metal binding1111Iron 1 By similarity
Metal binding1121Iron 1 By similarity
Metal binding2361Iron 2 By similarity
Metal binding2371Iron 2 By similarity
Metal binding2691Iron 2 By similarity
Metal binding2701Iron 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6FUV3 [UniParc].

Last modified July 19, 2004. Version 1.
Checksum: 45E5380F9C7D3AC2

FASTA32235,970
        10         20         30         40         50         60 
MSTNFEKHFE ENVDDCNLEQ LRDILVNKEG KSALANRFRA LFNLKTAASE FEANPSDAEK 

        70         80         90        100        110        120 
AVQYMGETFG DNSELLKHEV AYVLGQTKNL KAAPLLRKTM LDLAQQPMVR HEAAEALGAL 

       130        140        150        160        170        180 
GDKDSLEDLE KCLKNDPHVA VRETCELAIA RINWQHSDAP TKESLQQSLY SSIDPAPPLA 

       190        200        210        220        230        240 
LEKEYDLEEL KKLLNDQEKP LFLRYRAMFR LRDIGTDEAV LALASGFNDP SALFKHEIAY 

       250        260        270        280        290        300 
VFGQMGSTAA VPSLTEVLGR KEEAPMVRHE AAEALGAIAS EDALPILKQY LNDEVDVVRE 

       310        320 
SAIVALDMWE YENSNELEYA PA

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CR380952 Genomic DNA. Translation: CAG58915.1.
RefSeqXP_445991.1. XM_445991.1.

3D structure databases

ProteinModelPortalQ6FUV3.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2887799.
KEGGcgr:CAGL0F00407g.

Phylogenomic databases

HOGENOMHOG000248665.
KOK06072.
OMARIEWINS.
OrthoDBEOG4MSH78.

Enzyme and pathway databases

UniPathwayUPA00354.

Family and domain databases

Gene3D1.25.10.10. 1 hit.
HAMAPMF_03101. Deoxyhypusine_hydroxylase.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR027517. Deoxyhypusine_hydroxylase.
IPR004155. PBS_lyase_HEAT.
[Graphical view]
SMARTSM00567. EZ_HEAT. 5 hits.
[Graphical view]
SUPFAMSSF48371. ARM-type_fold. 1 hit.
PROSITEPS50077. HEAT_REPEAT. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDOHH_CANGA
AccessionPrimary (citable) accession number: Q6FUV3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: July 19, 2004
Last modified: May 29, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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