Reviewed, UniProtKB/Swiss-Prot Q6FU27 (LYS1_CANGA)

Names and origin

Protein names

Recommended name:
    Saccharopine dehydrogenase [NAD+, L-lysine-forming]
      Short name=SDH
    EC=1.5.1.7
Alternative name(s):
    Lysine--2-oxoglutarate reductase

Gene names

Name:	LYS1
Ordered Locus Names:	CAGL0F06875g

Organism

Candida glabrata (Yeast) (Torulopsis glabrata) [Complete proteome]

Taxonomic identifier

5478 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Nakaseomyces › mitosporic Nakaseomyces

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	N(6)-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O = L-lysine + 2-oxoglutarate + NADH.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungi route): step 3/3.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the AlaDH/PNT family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Lysine biosynthesis
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lysine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro saccharopine dehydrogenase (NAD+, L-lysine-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Saccharopine dehydrogenase [NAD+, L-lysine-forming]		PRO_0000199011
Sites
	Active site	204	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6FU27-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 9A578085727C3F2D	FASTA	372	41,552
	10 20 30 40 50 60 MSVTLHLRGE TKPLEHRAAL TPTTVKHLIG KGFKIYVEES PQSIFKIDEY RRAGAIIVPF 70 80 90 100 110 120 GSWISAPRDR IIIGLKEMPE EDKFPLVHEH IQFAHCYKDQ AGWKDVLRRF INGNGTLYDL 130 140 150 160 170 180 EFLEDDNGRR VAAFGFYAGF AGAALGLADW AFKQTHKDSE DLPAVSPYPN EKALIKDIGK 190 200 210 220 230 240 AYKNALKTGA KKPKVLIIGA LGRCGSGAID FLKKVGLPEE NIIKWDIQET SRGGPFPEIA 250 260 270 280 290 300 ASDIFINCIY LSKPIAPFIN YELLNKPDRK LRTVVDVSAD TTNPHNPIPI YNIATVFNKP 310 320 330 340 350 360 TVKVNTSSGP KLSVISIDHL PSLLPREASE FFAHDLLPSL EQLPSRHVSP VWVRAEKLFN 370 RHSARAIRES KL

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 2001 / CBS 138 / IFO 0622 / NRRL Y-65.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR380952 Genomic DNA. Translation: CAG59191.1.
RefSeq	XP_446267.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2887663.
KEGG	cgr:CAGL0F06875g.
Phylogenomic databases
HOGENOM	Q6FU27.
OMA	Q6FU27. IGALGRC.
Enzyme and pathway databases
BRENDA	1.5.1.7. 189220.
Family and domain databases
InterPro	IPR007698. Ala_DH/PNT_C. IPR007886. Ala_DH/PNT_N. [Graphical view]
Pfam	PF01262. AlaDh_PNT_C. 1 hit. PF05222. AlaDh_PNT_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYS1_CANGA

Accession

Primary (citable) accession number: Q6FU27

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	July 19, 2004
Last modified:	June 16, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)