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Protein

Enolase 1

Gene

ENO1

Organism
Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 1 (GPD1), Glyceraldehyde-3-phosphate dehydrogenase 2 (GPD2)
  2. Phosphoglycerate kinase (PGK1)
  3. Phosphoglycerate mutase (GPM1), Phosphoglycerate mutase (CAGL0K01705g)
  4. Enolase 1 (ENO1), Enolase 2 (ENO2)
  5. Pyruvate kinase 2 (PYK2), Pyruvate kinase 1 (PYK1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei160SubstrateBy similarity1
Binding sitei169SubstrateBy similarity1
Active sitei212Proton donorBy similarity1
Metal bindingi247MagnesiumBy similarity1
Metal bindingi296MagnesiumBy similarity1
Binding sitei296SubstrateBy similarity1
Metal bindingi321MagnesiumBy similarity1
Binding sitei321SubstrateBy similarity1
Active sitei346Proton acceptorBy similarity1
Binding sitei397SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase 1 (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase 1
2-phosphoglycerate dehydratase 1
Gene namesi
Name:ENO1
Ordered Locus Names:CAGL0F08261g
OrganismiCandida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
Taxonomic identifieri284593 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeNakaseomycesNakaseomyces/Candida clade
Proteomesi
  • UP000002428 Componenti: Chromosome F

Organism-specific databases

CGDiCAL0131204. CAGL0F08261g.
EuPathDBiFungiDB:CAGL0F08261g.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340441 – 438Enolase 1Add BLAST438

Proteomic databases

PRIDEiQ6FTW6.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi284593.XP_446328.1.

Structurei

3D structure databases

ProteinModelPortaliQ6FTW6.
SMRiQ6FTW6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni373 – 376Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

eggNOGiKOG2670. Eukaryota.
COG0148. LUCA.
HOGENOMiHOG000072174.
InParanoidiQ6FTW6.
KOiK01689.
OMAiAEERACN.
OrthoDBiEOG092C2W5X.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6FTW6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVTKVHART VYDSRGNPTV EVEVYTKDGM FRAIVPSGAS TGSHEALELR
60 70 80 90 100
DGDKSKWEGK GVTKAVSNVN DIIGPKLIES KLDVKDQKAI DEFMIKLDGT
110 120 130 140 150
KNKSKLGANA ILGVSLAVAR AGAAAKGVPL YQHIAELADM KKEPYVIPCP
160 170 180 190 200
FFNVLNGGVH AGGNLALQEF LIAPVGAESF HEALRLGSEV YHKLKALAKK
210 220 230 240 250
RIGSSAGNVG DEGGIAPSLS TPFEALDLIY DAIKEAGHEG KVKIAMDPAS
260 270 280 290 300
SEFFQGDKYD LDFKNPHPDA KNKLSGAQLG DYYKTILEKY PIVSLEDPFA
310 320 330 340 350
EDDWEAWTNF FPKAGVQIIA DDLTVTNPER IQTAIDKKTA DCLLLKVNQI
360 370 380 390 400
GSLTESINSA KLAYGAGWGV QVSHRSGETE DTFIADLVVG LRTGQIKSGS
410 420 430
LARSERLAKW NQILRIEEEL GASNTIFAGA KFHKGQLL
Length:438
Mass (Da):47,282
Last modified:July 19, 2004 - v1
Checksum:iA5C39636C234E43E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR380952 Genomic DNA. Translation: CAG59252.1.
RefSeqiXP_446328.1. XM_446328.1.

Genome annotation databases

EnsemblFungiiCAG59252; CAG59252; CAGL0F08261g.
GeneIDi2887972.
KEGGicgr:CAGL0F08261g.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiENO1_CANGA
AccessioniPrimary (citable) accession number: Q6FTW6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: July 19, 2004
Last modified: June 7, 2017
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families