Reviewed, UniProtKB/Swiss-Prot Q6FRB7 (IPYR_CANGA)

Names and origin

Protein names

Recommended name:
    Inorganic pyrophosphatase
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate phospho-hydrolase
      Short name=PPase

Gene names

Name:	IPP1
Ordered Locus Names:	CAGL0H09878g

Organism

Candida glabrata (Yeast) (Torulopsis glabrata) [Complete proteome]

Taxonomic identifier

5478 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Nakaseomyces › mitosporic Nakaseomyces

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate.
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 287	287	Inorganic pyrophosphatase		PRO_0000137581
Sites
	Metal binding	116	1	Magnesium 1 By similarity
	Metal binding	121	1	Magnesium 1 By similarity
	Metal binding	121	1	Magnesium 2 By similarity
	Metal binding	153	1	Magnesium 1 By similarity
	Binding site	79	1	Inorganic pyrophosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6FRB7-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: E4BEC0B55E442AAA	FASTA	287	32,313
	10 20 30 40 50 60 MVYTTRQIGA KNTLDYKVFI EEDGKPVSPF HDIPLYADKE ENIFNMVVEI PRWTNAKLEI 70 80 90 100 110 120 TKEETLNPII QDTKKGKLRY VRNCFPHHGY IHNYGAFPQT WEDPNQTHPE TKAVGDNDPV 130 140 150 160 170 180 DVLEIGETIG YTGQVKQVKV LGIMALLDEG ETDWKVIAID INDPLAPKLN DIEDVEKYFP 190 200 210 220 230 240 GLLRATNEWF RIYKIPDGKP ENQFAFSGEA KNKKYALDII KETNESWKQL IAGKSTDSKD 250 260 270 280 IALENTKVTD SPYYSASAAS AIPAAAPQAD APIDKSVDKW FFISGSA

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 2001 / CBS 138 / IFO 0622 / NRRL Y-65.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR380954 Genomic DNA. Translation: CAG60160.1.
RefSeq	XP_447227.1.
3D structure databases
SMR	Q6FRB7. Positions 3-286.
ModBase	Search...
Protein-protein interaction databases
STRING	Q6FRB7.
Genome annotation databases
GeneID	2888731.
GenomeReviews	Gene locus CAGL0H09878g in contig CR380954_GR.
KEGG	cgr:CAGL0H09878g.
Phylogenomic databases
eggNOG	fuNOG04419.
HOGENOM	HBG434750.
OMA	WFFISGS.
OrthoDB	EOG93JDNG.
PhylomeDB	Q6FRB7.
Enzyme and pathway databases
BRENDA	3.6.1.1. 189220.
Family and domain databases
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR_CANGA

Accession

Primary (citable) accession number: Q6FRB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	July 19, 2004
Last modified:	January 19, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)