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Q6FQF0

- UBP4_CANGA

UniProt

Q6FQF0 - UBP4_CANGA

Protein

Ubiquitin carboxyl-terminal hydrolase 4

Gene

DOA4

Organism
Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (19 Jul 2004)
      Previous versions | rss
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    Functioni

    Ubiquitin thioesterase that acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole. Removes also ubiquitin from soluble proteins targeted to proteasomes. Is essential to maintain a normal level of free ubiquitin. Required for promoting coordination of DNA replication and avoids DNA overreplication By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Enzyme regulationi

    RFU1 is an inhibitor of deubiquitination activity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei534 – 5341NucleophilePROSITE-ProRule annotation
    Active sitei842 – 8421Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. ubiquitinyl hydrolase activity Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 4 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 4
    Ubiquitin thioesterase 4
    Ubiquitin-specific-processing protease 4
    Gene namesi
    Name:DOA4
    Synonyms:UBP4
    Ordered Locus Names:CAGL0I06765g
    OrganismiCandida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
    Taxonomic identifieri284593 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeNakaseomycesmitosporic Nakaseomyces
    ProteomesiUP000002428: Chromosome I

    Subcellular locationi

    Cytoplasm By similarity. Late endosome membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. late endosome membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Endosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 887887Ubiquitin carboxyl-terminal hydrolase 4PRO_0000376819Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ6FQF0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini202 – 328127RhodanesePROSITE-ProRule annotationAdd
    BLAST
    Domaini525 – 885361USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 rhodanese domain.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Phylogenomic databases

    KOiK11839.
    OMAiEDLNQCG.
    OrthoDBiEOG7R2BSX.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR001763. Rhodanese-like_dom.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    SMARTiSM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52821. SSF52821. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    PS00972. USP_1. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6FQF0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPGIEQPVSR KNETLVKLSS LADEFVFNDE VQLNLQDVLQ ECVDTYQNYQ    50
    DEVKKIKNMD HTESEKVSEL CKSAYIYYKI VHNFITKVIP HLPEFEVATG 100
    PKASKLQAEL IKIYYSLFSR LESDKKISYI KNIIIKHMDT QENNHSVESH 150
    EQVKLSNKKL PVNRDAIEID KDSILQDIRY INGKRSGSGI SCSELLSLMK 200
    MKEDSLLLID VRPKLEYDAH HIKTKNIICI EPISFKESYS DQQIEKTSMI 250
    PSPKHEIQLF QRRSEFQYII LYTDLEEKSN FYFQQLKSLL EILLQRSFLR 300
    PIDDRKTKVL FLSDSLQNWI KNGGEIDKSQ EVSKIRNRSI SGSGPLLNSL 350
    SERKTIGAFP DINRNSTKQM PISPLPSLPG SERTVATPPN GSSTLGRINS 400
    PVTHYPKAPL INDSEFHLNI NNNHSPPTHL PSKDNNPLAS SMPIGSDHKP 450
    FMSPQNSLPL APKPPTLESK NYNFISDRSN IIDQKQNRSR SLEPQLPPIP 500
    STLIRKNSPE KTLSCNQMMD TSFTVGLENM GNSCYINCII QCIFATTELI 550
    KIFLNGTYAK HINKQSKLGS KGVLSHNFAK LLKDMYEENS SKKIGKKHGA 600
    VKTLQFKMAC ASVNSLFKDA SQQDCLEFCQ FLLDGLHEDL NQCGANPPLK 650
    ELSPEAEKMR ENLSLRVASS IEWERYLTTD FSIIVDLFQG QYASQLRCKV 700
    CNRTSTTYQA FSVLSVPVPS GKSCGLLDCF IEFTKTENLE VDEQWFCPSC 750
    KKKQPSTKKL TITRLPRNLI IHLKRFDNMM NKNNIFVRYP QILDLTPFWA 800
    NDSDGKLPPG ITDEIPARGQ VPPFNYRLYG AACHFGTLYG GHYTSYVDKG 850
    PEKGWIYFDD TVYRPVRFQN EFISPSAYVL FYHRITS 887
    Length:887
    Mass (Da):101,165
    Last modified:July 19, 2004 - v1
    Checksum:iA9E756B4D60894BE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR380955 Genomic DNA. Translation: CAG60481.1.
    RefSeqiXP_447544.1. XM_447544.1.

    Genome annotation databases

    GeneIDi2889068.
    KEGGicgr:CAGL0I06765g.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR380955 Genomic DNA. Translation: CAG60481.1 .
    RefSeqi XP_447544.1. XM_447544.1.

    3D structure databases

    ProteinModelPortali Q6FQF0.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2889068.
    KEGGi cgr:CAGL0I06765g.

    Phylogenomic databases

    KOi K11839.
    OMAi EDLNQCG.
    OrthoDBi EOG7R2BSX.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR001763. Rhodanese-like_dom.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    SMARTi SM00450. RHOD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52821. SSF52821. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    PS00972. USP_1. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Entry informationi

    Entry nameiUBP4_CANGA
    AccessioniPrimary (citable) accession number: Q6FQF0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2009
    Last sequence update: July 19, 2004
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3