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Q6FQF0 (UBP4_CANGA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 4

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 4
Ubiquitin thioesterase 4
Ubiquitin-specific-processing protease 4
Gene names
Name:DOA4
Synonyms:UBP4
Ordered Locus Names:CAGL0I06765g
OrganismCandida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata) [Complete proteome]
Taxonomic identifier284593 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeNakaseomycesmitosporic Nakaseomyces

Protein attributes

Sequence length887 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Ubiquitin thioesterase that acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole. Removes also ubiquitin from soluble proteins targeted to proteasomes. Is essential to maintain a normal level of free ubiquitin. Required for promoting coordination of DNA replication and avoids DNA overreplication By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulation

RFU1 is an inhibitor of deubiquitination activity By similarity.

Subcellular location

Cytoplasm By similarity. Late endosome membrane; Peripheral membrane protein By similarity.

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 rhodanese domain.

Contains 1 USP domain.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Cellular componentCytoplasm
Endosome
Membrane
   Molecular functionHydrolase
Protease
Thiol protease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentlate endosome membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncysteine-type peptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 887887Ubiquitin carboxyl-terminal hydrolase 4
PRO_0000376819

Regions

Domain202 – 328127Rhodanese
Domain525 – 885361USP

Sites

Active site5341Nucleophile By similarity
Active site8421Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6FQF0 [UniParc].

Last modified July 19, 2004. Version 1.
Checksum: A9E756B4D60894BE

FASTA887101,165
        10         20         30         40         50         60 
MPGIEQPVSR KNETLVKLSS LADEFVFNDE VQLNLQDVLQ ECVDTYQNYQ DEVKKIKNMD 

        70         80         90        100        110        120 
HTESEKVSEL CKSAYIYYKI VHNFITKVIP HLPEFEVATG PKASKLQAEL IKIYYSLFSR 

       130        140        150        160        170        180 
LESDKKISYI KNIIIKHMDT QENNHSVESH EQVKLSNKKL PVNRDAIEID KDSILQDIRY 

       190        200        210        220        230        240 
INGKRSGSGI SCSELLSLMK MKEDSLLLID VRPKLEYDAH HIKTKNIICI EPISFKESYS 

       250        260        270        280        290        300 
DQQIEKTSMI PSPKHEIQLF QRRSEFQYII LYTDLEEKSN FYFQQLKSLL EILLQRSFLR 

       310        320        330        340        350        360 
PIDDRKTKVL FLSDSLQNWI KNGGEIDKSQ EVSKIRNRSI SGSGPLLNSL SERKTIGAFP 

       370        380        390        400        410        420 
DINRNSTKQM PISPLPSLPG SERTVATPPN GSSTLGRINS PVTHYPKAPL INDSEFHLNI 

       430        440        450        460        470        480 
NNNHSPPTHL PSKDNNPLAS SMPIGSDHKP FMSPQNSLPL APKPPTLESK NYNFISDRSN 

       490        500        510        520        530        540 
IIDQKQNRSR SLEPQLPPIP STLIRKNSPE KTLSCNQMMD TSFTVGLENM GNSCYINCII 

       550        560        570        580        590        600 
QCIFATTELI KIFLNGTYAK HINKQSKLGS KGVLSHNFAK LLKDMYEENS SKKIGKKHGA 

       610        620        630        640        650        660 
VKTLQFKMAC ASVNSLFKDA SQQDCLEFCQ FLLDGLHEDL NQCGANPPLK ELSPEAEKMR 

       670        680        690        700        710        720 
ENLSLRVASS IEWERYLTTD FSIIVDLFQG QYASQLRCKV CNRTSTTYQA FSVLSVPVPS 

       730        740        750        760        770        780 
GKSCGLLDCF IEFTKTENLE VDEQWFCPSC KKKQPSTKKL TITRLPRNLI IHLKRFDNMM 

       790        800        810        820        830        840 
NKNNIFVRYP QILDLTPFWA NDSDGKLPPG ITDEIPARGQ VPPFNYRLYG AACHFGTLYG 

       850        860        870        880 
GHYTSYVDKG PEKGWIYFDD TVYRPVRFQN EFISPSAYVL FYHRITS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR380955 Genomic DNA. Translation: CAG60481.1.
RefSeqXP_447544.1. XM_447544.1.

3D structure databases

ProteinModelPortalQ6FQF0.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2889068.
KEGGcgr:CAGL0I06765g.

Phylogenomic databases

KOK11839.
OMAEDLNQCG.
OrthoDBEOG7R2BSX.

Family and domain databases

Gene3D3.40.250.10. 1 hit.
InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR001763. Rhodanese-like_dom.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00443. UCH. 1 hit.
[Graphical view]
SMARTSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMSSF52821. SSF52821. 1 hit.
PROSITEPS50206. RHODANESE_3. 1 hit.
PS00972. USP_1. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBP4_CANGA
AccessionPrimary (citable) accession number: Q6FQF0
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: July 19, 2004
Last modified: April 16, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries