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Q6FQF0

- UBP4_CANGA

UniProt

Q6FQF0 - UBP4_CANGA

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Protein
Ubiquitin carboxyl-terminal hydrolase 4
Gene
DOA4, UBP4, CAGL0I06765g
Organism
Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Ubiquitin thioesterase that acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole. Removes also ubiquitin from soluble proteins targeted to proteasomes. Is essential to maintain a normal level of free ubiquitin. Required for promoting coordination of DNA replication and avoids DNA overreplication By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulationi

RFU1 is an inhibitor of deubiquitination activity By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei534 – 5341Nucleophile By similarity
Active sitei842 – 8421Proton acceptor By similarity

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. ubiquitinyl hydrolase activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 4 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 4
Ubiquitin thioesterase 4
Ubiquitin-specific-processing protease 4
Gene namesi
Name:DOA4
Synonyms:UBP4
Ordered Locus Names:CAGL0I06765g
OrganismiCandida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
Taxonomic identifieri284593 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeNakaseomycesmitosporic Nakaseomyces
ProteomesiUP000002428: Chromosome I

Subcellular locationi

GO - Cellular componenti

  1. late endosome membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 887887Ubiquitin carboxyl-terminal hydrolase 4
PRO_0000376819Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ6FQF0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini202 – 328127Rhodanese
Add
BLAST
Domaini525 – 885361USP
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.
Contains 1 rhodanese domain.
Contains 1 USP domain.

Phylogenomic databases

KOiK11839.
OMAiEDLNQCG.
OrthoDBiEOG7R2BSX.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR001763. Rhodanese-like_dom.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
PS00972. USP_1. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6FQF0-1 [UniParc]FASTAAdd to Basket

« Hide

MPGIEQPVSR KNETLVKLSS LADEFVFNDE VQLNLQDVLQ ECVDTYQNYQ    50
DEVKKIKNMD HTESEKVSEL CKSAYIYYKI VHNFITKVIP HLPEFEVATG 100
PKASKLQAEL IKIYYSLFSR LESDKKISYI KNIIIKHMDT QENNHSVESH 150
EQVKLSNKKL PVNRDAIEID KDSILQDIRY INGKRSGSGI SCSELLSLMK 200
MKEDSLLLID VRPKLEYDAH HIKTKNIICI EPISFKESYS DQQIEKTSMI 250
PSPKHEIQLF QRRSEFQYII LYTDLEEKSN FYFQQLKSLL EILLQRSFLR 300
PIDDRKTKVL FLSDSLQNWI KNGGEIDKSQ EVSKIRNRSI SGSGPLLNSL 350
SERKTIGAFP DINRNSTKQM PISPLPSLPG SERTVATPPN GSSTLGRINS 400
PVTHYPKAPL INDSEFHLNI NNNHSPPTHL PSKDNNPLAS SMPIGSDHKP 450
FMSPQNSLPL APKPPTLESK NYNFISDRSN IIDQKQNRSR SLEPQLPPIP 500
STLIRKNSPE KTLSCNQMMD TSFTVGLENM GNSCYINCII QCIFATTELI 550
KIFLNGTYAK HINKQSKLGS KGVLSHNFAK LLKDMYEENS SKKIGKKHGA 600
VKTLQFKMAC ASVNSLFKDA SQQDCLEFCQ FLLDGLHEDL NQCGANPPLK 650
ELSPEAEKMR ENLSLRVASS IEWERYLTTD FSIIVDLFQG QYASQLRCKV 700
CNRTSTTYQA FSVLSVPVPS GKSCGLLDCF IEFTKTENLE VDEQWFCPSC 750
KKKQPSTKKL TITRLPRNLI IHLKRFDNMM NKNNIFVRYP QILDLTPFWA 800
NDSDGKLPPG ITDEIPARGQ VPPFNYRLYG AACHFGTLYG GHYTSYVDKG 850
PEKGWIYFDD TVYRPVRFQN EFISPSAYVL FYHRITS 887
Length:887
Mass (Da):101,165
Last modified:July 19, 2004 - v1
Checksum:iA9E756B4D60894BE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR380955 Genomic DNA. Translation: CAG60481.1.
RefSeqiXP_447544.1. XM_447544.1.

Genome annotation databases

GeneIDi2889068.
KEGGicgr:CAGL0I06765g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR380955 Genomic DNA. Translation: CAG60481.1 .
RefSeqi XP_447544.1. XM_447544.1.

3D structure databases

ProteinModelPortali Q6FQF0.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2889068.
KEGGi cgr:CAGL0I06765g.

Phylogenomic databases

KOi K11839.
OMAi EDLNQCG.
OrthoDBi EOG7R2BSX.

Family and domain databases

Gene3Di 3.40.250.10. 1 hit.
InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR001763. Rhodanese-like_dom.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
[Graphical view ]
SMARTi SM00450. RHOD. 1 hit.
[Graphical view ]
SUPFAMi SSF52821. SSF52821. 1 hit.
PROSITEi PS50206. RHODANESE_3. 1 hit.
PS00972. USP_1. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Entry informationi

Entry nameiUBP4_CANGA
AccessioniPrimary (citable) accession number: Q6FQF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: July 19, 2004
Last modified: September 3, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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