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Protein

Ubiquitin carboxyl-terminal hydrolase 4

Gene

DOA4

Organism
Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Ubiquitin thioesterase that acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole. Removes also ubiquitin from soluble proteins targeted to proteasomes. Is essential to maintain a normal level of free ubiquitin. Required for promoting coordination of DNA replication and avoids DNA overreplication (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulationi

RFU1 is an inhibitor of deubiquitination activity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei534 – 5341NucleophilePROSITE-ProRule annotation
Active sitei842 – 8421Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 4 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 4
Ubiquitin thioesterase 4
Ubiquitin-specific-processing protease 4
Gene namesi
Name:DOA4
Synonyms:UBP4
Ordered Locus Names:CAGL0I06765g
OrganismiCandida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
Taxonomic identifieri284593 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeNakaseomycesNakaseomyces/Candida clade
ProteomesiUP000002428 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:CAGL0I06765g.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 887887Ubiquitin carboxyl-terminal hydrolase 4PRO_0000376819Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi284593.XP_447544.1.

Structurei

3D structure databases

ProteinModelPortaliQ6FQF0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini202 – 328127RhodanesePROSITE-ProRule annotationAdd
BLAST
Domaini525 – 885361USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 rhodanese domain.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Phylogenomic databases

InParanoidiQ6FQF0.
KOiK11839.
OMAiEDLNQCG.
OrthoDBiEOG7R2BSX.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR001763. Rhodanese-like_dom.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
PS00972. USP_1. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6FQF0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGIEQPVSR KNETLVKLSS LADEFVFNDE VQLNLQDVLQ ECVDTYQNYQ
60 70 80 90 100
DEVKKIKNMD HTESEKVSEL CKSAYIYYKI VHNFITKVIP HLPEFEVATG
110 120 130 140 150
PKASKLQAEL IKIYYSLFSR LESDKKISYI KNIIIKHMDT QENNHSVESH
160 170 180 190 200
EQVKLSNKKL PVNRDAIEID KDSILQDIRY INGKRSGSGI SCSELLSLMK
210 220 230 240 250
MKEDSLLLID VRPKLEYDAH HIKTKNIICI EPISFKESYS DQQIEKTSMI
260 270 280 290 300
PSPKHEIQLF QRRSEFQYII LYTDLEEKSN FYFQQLKSLL EILLQRSFLR
310 320 330 340 350
PIDDRKTKVL FLSDSLQNWI KNGGEIDKSQ EVSKIRNRSI SGSGPLLNSL
360 370 380 390 400
SERKTIGAFP DINRNSTKQM PISPLPSLPG SERTVATPPN GSSTLGRINS
410 420 430 440 450
PVTHYPKAPL INDSEFHLNI NNNHSPPTHL PSKDNNPLAS SMPIGSDHKP
460 470 480 490 500
FMSPQNSLPL APKPPTLESK NYNFISDRSN IIDQKQNRSR SLEPQLPPIP
510 520 530 540 550
STLIRKNSPE KTLSCNQMMD TSFTVGLENM GNSCYINCII QCIFATTELI
560 570 580 590 600
KIFLNGTYAK HINKQSKLGS KGVLSHNFAK LLKDMYEENS SKKIGKKHGA
610 620 630 640 650
VKTLQFKMAC ASVNSLFKDA SQQDCLEFCQ FLLDGLHEDL NQCGANPPLK
660 670 680 690 700
ELSPEAEKMR ENLSLRVASS IEWERYLTTD FSIIVDLFQG QYASQLRCKV
710 720 730 740 750
CNRTSTTYQA FSVLSVPVPS GKSCGLLDCF IEFTKTENLE VDEQWFCPSC
760 770 780 790 800
KKKQPSTKKL TITRLPRNLI IHLKRFDNMM NKNNIFVRYP QILDLTPFWA
810 820 830 840 850
NDSDGKLPPG ITDEIPARGQ VPPFNYRLYG AACHFGTLYG GHYTSYVDKG
860 870 880
PEKGWIYFDD TVYRPVRFQN EFISPSAYVL FYHRITS
Length:887
Mass (Da):101,165
Last modified:July 19, 2004 - v1
Checksum:iA9E756B4D60894BE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR380955 Genomic DNA. Translation: CAG60481.1.
RefSeqiXP_447544.1. XM_447544.1.

Genome annotation databases

GeneIDi2889068.
KEGGicgr:CAGL0I06765g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR380955 Genomic DNA. Translation: CAG60481.1.
RefSeqiXP_447544.1. XM_447544.1.

3D structure databases

ProteinModelPortaliQ6FQF0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi284593.XP_447544.1.

Protein family/group databases

MEROPSiC19.005.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2889068.
KEGGicgr:CAGL0I06765g.

Organism-specific databases

EuPathDBiFungiDB:CAGL0I06765g.

Phylogenomic databases

InParanoidiQ6FQF0.
KOiK11839.
OMAiEDLNQCG.
OrthoDBiEOG7R2BSX.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR001763. Rhodanese-like_dom.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
PS00972. USP_1. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBP4_CANGA
AccessioniPrimary (citable) accession number: Q6FQF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: July 19, 2004
Last modified: June 24, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.