Q6FQB2 (TRM10_CANGA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine(9)-N1)-methyltransferase
EC=2.1.1.221

Alternative name(s):
tRNA methyltransferase 10
tRNA(m1G9)-methyltransferase
Short name=tRNA(m1G9)MTase

Gene names

Name:	TRM10
Ordered Locus Names:	CAGL0I07667g

Organism

Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata) [Complete proteome]

Taxonomic identifier

284593 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Nakaseomyces › mitosporic Nakaseomyces

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(1)-methylguanine at position 9 (m1G9) in cytoplasmic tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + guanine9 in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine9 in tRNA.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family. TRM10 subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 287	287	tRNA (guanine(9)-N1)-methyltransferase		PRO_0000060512
Regions
	Compositional bias	278 – 287	10	Poly-Gln

Sequences

	Sequence		Length	Mass (Da)
	Q6FQB2 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 5D9C32BFB5AB7D41	FASTA	287	33,876
	10 20 30 40 50 60 MSDTSDLVDG KWQRLPPVPE GMSKSQWKKQ WRRQMYEAKK EEYAEVRKEK RKRAKENRRK 70 80 90 100 110 120 KIQEYIDRGE EVPAELKREP RVNRDQVASG INIILDCAFD DLMNDKEIVS TSNQITRAYS 130 140 150 160 170 180 ANRRASQYAN ITVTSFNKRL KERFDKALDD CNYPQWQNFK FVSDEKLITE GDKSKMVYLT 190 200 210 220 230 240 ADTEEQLDTL EPGMTYIVGG IVDKNRHKLL CYNKAKELGI PTRRLPIGEY IKIEGRKVLT 250 260 270 280 TTHVIQLMLK YCESRDWKES FESVIPSRKL DPVKEKEQQQ QQQQQQQ

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR380955 Genomic DNA. Translation: CAG60519.1.
RefSeq	XP_447582.1. XM_447582.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2889002.
GenomeReviews	Gene locus CAGL0I07667g in contig CR380955_GR.
KEGG	cgr:CAGL0I07667g.
Phylogenomic databases
eggNOG	fuNOG04600.
HOGENOM	HBG717479.
OMA	SNVERKQ.
OrthoDB	EOG48D43Z.
Family and domain databases
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR007356. tRNA_m1G_MeTrfase_euk. IPR016653. tRNA_m1G_mtfrase_met. [Graphical view]
KO	K15445.
PANTHER	PTHR13563. DUF425. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF016323. tRNA_m1G_mtfrase_met. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRM10_CANGA

Accession

Primary (citable) accession number: Q6FQB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program