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Q6FPY0

- LIPA_CANGA

UniProt

Q6FPY0 - LIPA_CANGA

Protein

Lipoyl synthase, mitochondrial

Gene

CAGL0I10923g

Organism
Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (19 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi120 – 1201Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi125 – 1251Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi131 – 1311Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi151 – 1511Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi155 – 1551Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi158 – 1581Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    Ordered Locus Names:CAGL0I10923g
    OrganismiCandida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
    Taxonomic identifieri284593 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeNakaseomycesmitosporic Nakaseomyces
    ProteomesiUP000002428: Chromosome I

    Subcellular locationi

    Mitochondrion UniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 1818MitochondrionUniRule annotationAdd
    BLAST
    Chaini19 – 388370Lipoyl synthase, mitochondrialPRO_0000398260Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ6FPY0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    KOiK03644.
    OMAiPEEPYNT.
    OrthoDBiEOG79KPR7.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q6FPY0-1 [UniParc]FASTAAdd to Basket

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    MRLTTVQRRF LVSTKAKVSG ASISSTANTG SASAGAPNGQ TRRRRRITEF    50
    KDALNLGPSF EDFVSGRAAG FTVVDPLEQM RQDREEYKKL PKWLKVPIPK 100
    GVNYHKLKKD VKELKLSTVC EEARCPNIGE CWGGKDKSKA TATIMLLGDT 150
    CTRGCRFCSV KTNRNPAKPD PTEPENTAEA ISRWGLGYVV LTTVDRDDLP 200
    DGGAHHLAET VIKIKQKAPK TLVEALTGDF LGNLEMVDVM AKSGLDVYAH 250
    NLETVEALTP HVRDRRAGYR QSLNVLKRAK ETVPSLITKT SVMLGLGETD 300
    DQIIQTMQDL RAINCDVITF GQYMRPTKRH MKVVEYVRPE KFDYWKDKAK 350
    EMGFLYCASG PLVRSSYKAG EAFIENVLNK RKQDNKRI 388
    Length:388
    Mass (Da):43,401
    Last modified:July 19, 2004 - v1
    Checksum:i457598F13CF7B740
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR380955 Genomic DNA. Translation: CAG60659.1.
    RefSeqiXP_447714.1. XM_447714.1.

    Genome annotation databases

    GeneIDi2889354.
    KEGGicgr:CAGL0I10923g.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR380955 Genomic DNA. Translation: CAG60659.1 .
    RefSeqi XP_447714.1. XM_447714.1.

    3D structure databases

    ProteinModelPortali Q6FPY0.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2889354.
    KEGGi cgr:CAGL0I10923g.

    Phylogenomic databases

    KOi K03644.
    OMAi PEEPYNT.
    OrthoDBi EOG79KPR7.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Entry informationi

    Entry nameiLIPA_CANGA
    AccessioniPrimary (citable) accession number: Q6FPY0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: July 19, 2004
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3