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Q6FNR1 (TRMB_CANGA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:TRM8
Ordered Locus Names:CAGL0J09746g
OrganismCandida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata) [Complete proteome]
Taxonomic identifier284593 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeNakaseomycesmitosporic Nakaseomyces

Protein attributes

Sequence length285 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subunit structure

Forms a complex with TRM82 By similarity. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
tRNA-binding
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 285285tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_03055
PRO_0000370592

Regions

Region125 – 1262S-adenosyl-L-methionine binding By similarity
Region160 – 1612S-adenosyl-L-methionine binding By similarity
Region258 – 2603S-adenosyl-L-methionine binding By similarity

Sites

Active site1831 By similarity
Binding site1021S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1801S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6FNR1 [UniParc].

Last modified July 19, 2004. Version 1.
Checksum: D2773B50B1CB2FD9

FASTA28533,406
        10         20         30         40         50         60 
MKAKPLSNDP GSKRYAYRVN KEENRKELKH VKIDESSLVE GHQVDLPKKR FYRQRAHSNP 

        70         80         90        100        110        120 
FSDHQLEYPG SPEEMDWTKL YPHYVDPESG KMTKKVTIAD IGCGFGGLLV DLSPEFPDDL 

       130        140        150        160        170        180 
ILGMEIRVQV TNYVEDRIIA LRTNHVKEQG YQNINVLRGN AMKFLPNFFQ KGQLSKMFFC 

       190        200        210        220        230        240 
FPDPHFKQRK HKARIITNTL LSEYAYVLRE GGIVYTITDV KDLHDWMVKH LTEHPLFERL 

       250        260        270        280 
PEEWEEEDSC VRIMRHATEE GKKVERKKGD KFVACFRRLP TPEII 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR380956 Genomic DNA. Translation: CAG61084.1.
RefSeqXP_448133.1. XM_448133.1.

3D structure databases

ProteinModelPortalQ6FNR1.
SMRQ6FNR1. Positions 59-285.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2889721.
KEGGcgr:CAGL0J09746g.

Phylogenomic databases

HOGENOMHOG000260965.
KOK03439.
OMARAHSNPI.
OrthoDBEOG708W9T.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_CANGA
AccessionPrimary (citable) accession number: Q6FNR1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: July 19, 2004
Last modified: April 16, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways