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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

TRM8

Organism
Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei102 – 1021S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei180 – 1801S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Active sitei183 – 1831UniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
  2. tRNA binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Transfer RNA methyltransferase 8UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:TRM8UniRule annotation
Ordered Locus Names:CAGL0J09746g
OrganismiCandida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata)
Taxonomic identifieri284593 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeNakaseomycesNakaseomyces/Candida clade
ProteomesiUP000002428: Chromosome J

Subcellular locationi

Nucleus UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 285285tRNA (guanine-N(7)-)-methyltransferasePRO_0000370592Add
BLAST

Interactioni

Subunit structurei

Forms a complex with TRM82.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ6FNR1.
SMRiQ6FNR1. Positions 59-285.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1262S-adenosyl-L-methionine bindingUniRule annotation
Regioni160 – 1612S-adenosyl-L-methionine bindingUniRule annotation
Regioni258 – 2603S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000260965.
InParanoidiQ6FNR1.
KOiK03439.
OMAiMCTHFEE.
OrthoDBiEOG708W9T.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6FNR1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAKPLSNDP GSKRYAYRVN KEENRKELKH VKIDESSLVE GHQVDLPKKR
60 70 80 90 100
FYRQRAHSNP FSDHQLEYPG SPEEMDWTKL YPHYVDPESG KMTKKVTIAD
110 120 130 140 150
IGCGFGGLLV DLSPEFPDDL ILGMEIRVQV TNYVEDRIIA LRTNHVKEQG
160 170 180 190 200
YQNINVLRGN AMKFLPNFFQ KGQLSKMFFC FPDPHFKQRK HKARIITNTL
210 220 230 240 250
LSEYAYVLRE GGIVYTITDV KDLHDWMVKH LTEHPLFERL PEEWEEEDSC
260 270 280
VRIMRHATEE GKKVERKKGD KFVACFRRLP TPEII
Length:285
Mass (Da):33,406
Last modified:July 19, 2004 - v1
Checksum:iD2773B50B1CB2FD9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR380956 Genomic DNA. Translation: CAG61084.1.
RefSeqiXP_448133.1. XM_448133.1.

Genome annotation databases

GeneIDi2889721.
KEGGicgr:CAGL0J09746g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR380956 Genomic DNA. Translation: CAG61084.1.
RefSeqiXP_448133.1. XM_448133.1.

3D structure databases

ProteinModelPortaliQ6FNR1.
SMRiQ6FNR1. Positions 59-285.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2889721.
KEGGicgr:CAGL0J09746g.

Phylogenomic databases

HOGENOMiHOG000260965.
InParanoidiQ6FNR1.
KOiK03439.
OMAiMCTHFEE.
OrthoDBiEOG708W9T.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_CANGA
AccessioniPrimary (citable) accession number: Q6FNR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: July 19, 2004
Last modified: January 7, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.