Q6FKD4 (PHO85_CANGA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Negative regulator of the PHO system
EC=2.7.11.22

Alternative name(s):
Serine/threonine-protein kinase PHO85

Gene names

Name:	PHO85
Ordered Locus Names:	CAGL0L12474g

Organism

Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) (Yeast) (Torulopsis glabrata) [Complete proteome]

Taxonomic identifier

284593 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Nakaseomyces › mitosporic Nakaseomyces › Candida glabrata

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	When phosphate concentrations are high it phosphorylates the PHO4 transcription factor thus establishing repression By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with a number of cyclins By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: GOC
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	Negative regulator of the PHO system		PRO_0000086517
Regions
	Domain	6 – 296	291	Protein kinase
	Nucleotide binding	12 – 20	9	ATP By similarity
Sites
	Active site	132	1	Proton acceptor By similarity
	Binding site	35	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6FKD4 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 79706E049D13B7B5	FASTA	302	34,488
	10 20 30 40 50 60 MSSSQFKQLE KLGNGTYATV YKGLNKSTGV YVALKEVKLD SEEGTPSTAI REISLMKELK 70 80 90 100 110 120 HDNIVRLYDV IHTENKLTLV FEYMDNDLKK YMDSRTVGNA PRGLEMNLVK YFQWQLLEGL 130 140 150 160 170 180 AFCHENKILH RDLKPQNLLI TKRGQLKLGD FGLARAFGIP VNTFSSEVVT LWYRAPDVLM 190 200 210 220 230 240 GSRTYSTSID IWSCGCILAE MITGKPLFPG TNDEEQLKLI FDKMGTPNET TWPGVTSLPK 250 260 270 280 290 300 YNPNFQQRLP KDLKAELQPY VKEPLDDNVI DLLHGLLQLN PDMRLSAKQA LLHPWFSEYY ES

References

[1]

"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR380958 Genomic DNA. Translation: CAG62284.1.
RefSeq	XP_449310.1. XM_449310.1.
3D structure databases
ProteinModelPortal	Q6FKD4.
SMR	Q6FKD4. Positions 18-300.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2890701.
KEGG	cgr:CAGL0L12474g.
Phylogenomic databases
KO	K06655.
OMA	FRAYNFT.
OrthoDB	EOG4QJVX0.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHO85_CANGA

Accession

Primary (citable) accession number: Q6FKD4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	July 19, 2004
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program